Residual and Antimicrobial Activity of Final Irrigation Protocols on Enterococcus Faecalis Biofilm in Dentin

Baca, Pilar; Junco, Pilar; Arias-Moliz, María Teresa; González-Rodrı́guez, Marı́a Paloma; Ferrer-Luque, Carmen Marı́a

doi:10.1016/j.joen.2010.11.036

Cited by 65 publications

(97 citation statements)

References 30 publications

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“…Nevertheless, the exact concentration used clinically is not usually precisely reported 4,5,12,39) . CHX and CTR have proven to be effective against E. faecalis, alone 41) or in combination 42) when used as irrigating solutions, which is in line with the results of this study, where DBP+0.2% CHX paste, DBP+0.2% CTR paste and DBP+0.2% CHX+0.2% CTR paste exhibited NG of E. faecalis at both experimental periods. In clinical situations, Nagata et al 19) have reported that a paste composed of Ca(OH)2+2% CHX gel is as effective as TAP.…”

Section: Discussionsupporting

confidence: 91%

Antibacterial efficacy of several intracanal medicaments for endodontic therapy

et al. 2017

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The antibacterial efficacy of intracanal medicaments for 2 and 7 days, in open apex root canals contaminated with Enterococcus faecalis biofilms was compared. One hundred and thirty-eight standardized uniradicular human roots were inoculated with E. faecalis. Colony forming units (CFU) were recorded before and after medication. Samples were divided in two (2/7 days), and subdivided (n=8/group): triple antibiotic paste (TAP); double antibiotic paste (DAP); dental base paste (DBP), consisting of calcium hydroxide, propylene glycol and calcium carbonate; DBP+0.2% chlorhexidine (CHX); DBP+0.2% cetrimide (CTR); DBP+0.2% CHX+0.2% CTR; 2.5% sodium hypochlorite (NaOCl); and distilled water. At both periods TAP, DAP, DBP+0.2% CHX, DBP+0.2% CTR and DBP+0.2% CHX+0.2% CTR exhibited no growth (NG) of E. faecalis. Distilled water and 2.5% NaOCl obtained the lowest CFU reduction. No growth with DBP was reached only at 7 days. CHX and/or CTR pastes were as effective as DAP and TAP. A 2-day period provided an effective disinfection.

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Section: Discussionsupporting

confidence: 91%

Antibacterial efficacy of several intracanal medicaments for endodontic therapy

et al. 2017

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“…Bovine dentine specimens were selected because they are a satisfactory substratum for bacterial adhesion and biofi lm formation. Based on a previous model that uses human dentine specimens [23], we designed a root dentine-volumetric test as a carrier to test the antimicrobial effi cacy of solvents against biofi lms. The specimens were easily size-standardized and infected, and they were simple to manipulate.…”

Section: Discussionmentioning

confidence: 99%

“…It is known that 0.2% CTR, used alone [21] or in combination with chelating agents [22] or chlorhexidine [23], has the capacity to eradicate E. faecalis biofi lm. When the essential oils were combined with lower concentrations of CTR (0.05% and 0.1%), they effectively eradicated the biofi lms, probably due to an enhancement of the antimicrobial properties of the essentials oils, mainly attributed to lipophilic compounds -terpenoids and phenolics [15].…”

Section: Discussionmentioning

confidence: 99%

“…Each slice was cut into four equal sections that were then adjusted in size using a calibrator and polished with 150, 220, and 600 grit silicon carbide papers in ascending order. Twelve dentine blocks were obtained from each root, and the smear layer formed during preparation of the dentine blocks was eliminated by submerging them in 17% EDTA for 2 min and then in 2.5% NaOCl for 1 min to standardize the specimens before their sterilization and separation into the different groups for study [23]. After sterilization, the specimens were in- Fig.…”

Section: Preparation Of Dentine Blocksmentioning

confidence: 99%

“…It also has the capability of eradicating E. faecalis bio-fi lms in vitro [21]. CTR has shown benefi cial bactericidal activity in combination with EDTA, citric acid, and chlorhexidine against biofi lm cultures [22,23]. Recently, Pappen et al [24] demonstrated that CTR improves the antimicrobial properties of Tetraclean and MTAD against E. faecalis.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial activity of essential oils and chloroform alone and combinated with cetrimide againstEnterococcus faecalisbiofilm

Martos¹,

Ferrer-Luque²,

González-Rodrı́guez³

et al. 2013

European Journal of Microbiology and Immunology

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The Enterococcus faecalis bacteria have been identified as the most commonly recovered species from teeth with persistent endodontic infections. The antimicrobial activity of essential oils and chloroform (CHL), alone and in association with various concentrations of cetrimide (CTR), against biofilm of Enterococcus faecalis was investigated. Solutions of CHL, eucalyptus oil (EO) and orange oil (OO) associated with CTR at 0.3%, 0.2%, 0.1%, and 0.05% were used to determine antimicrobial activity by exposing treated bovine dentine blocks to E. faecalis. Biofilms grown in the dentine blocks for 7 days were exposed to solutions for 2 and 5 min. Biofilm reduction between OO and EO at 2 min did not show any significant differences; however, OO had a higher kill percentage of biofilms than did the eucalyptus oil at 5 min (p < 0.01). Combinations with CTR at all concentrations achieved a 100% kill rate at 2 and 5 min. The association of CTR with solvent agents achieved the maximum antimicrobial activity against E. faecalis biofilms in dentine.

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Antibacterial efficacy of silver diamine fluoride as a root canal irrigant

Al‐Madi

Al‐Jamie

Al-Owaid

et al. 2019

Clinical & Exp Dental Res

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ObjectivesConventional disinfectants and medicaments have not been able to achieve and maintain sterilization of root canals. The purpose of the study was to evaluate the antibacterial efficacy of 3.8% silver diamine fluoride (SDF) in comparison with 2% chlorhexidine (CHX) against Enterococcus faecalis biofilm.Materials and methodsExtracted human teeth were used to make 70 dentin discs that were then inoculated with E. faecalis to generate a 3‐week‐old biofilm model. The discs were subjected to treatment with 3.8% SDF, 2% CHX, sodium hypochlorite (NaOCl), or saline for 10 min. After exposure, the dentin discs were examined with a confocal laser scanning microscope to verify the percentage of live versus dead cells within the biofilm. Univariate one‐way analysis of variance and Tukey‐honestly significant difference post hoc analysis (p < .05) were performed to detect significant differences.Results and conclusionThe NaOCl group showed the greatest percentage of dead cells (62.26%) among all groups (p < .05). The SDF group showed a significantly higher percentage of dead cells (57.39%) than the 2% CHX and saline groups (p < .05). SDF possessed higher antimicrobial activity than 2% CHX against E. faecalis biofilms.

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Residual and Antimicrobial Activity of Final Irrigation Protocols on Enterococcus Faecalis Biofilm in Dentin

Cited by 65 publications

References 30 publications

Antibacterial efficacy of several intracanal medicaments for endodontic therapy

Antibacterial efficacy of several intracanal medicaments for endodontic therapy

Antimicrobial activity of essential oils and chloroform alone and combinated with cetrimide againstEnterococcus faecalisbiofilm

Antibacterial efficacy of silver diamine fluoride as a root canal irrigant

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