A perfusable vascularized full-thickness skin model for potential topical and systemic applications

Salameh, Sacha; Tissot, Nicolas; Cache, Kevin; Lima, Joaquim; Suzuki, Itaru; Marinho, Paulo A.; Rielland, Maïté; Sœur, Jérémie; Takeuchi, Shoji; Germain, Stéphane; Breton, Lionel

doi:10.1088/1758-5090/abfca8

Cited by 33 publications

(37 citation statements)

References 89 publications

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“…The vascularization studies carried out on microfluidic devices culminated in the engineering of networks of perfusable cellular microvessels, [260] which were realized by precisely controlling the vascular microenvironment cues in vitro. [295][296][297] In certain models, the survival of the surrounding tissue entirely depends on the nutrients supplied by these vessels. In 2016, Sobrino et al generated vascularized 3D microtumors in an "on-a-chip" platform.…”

Section: Tissue Vascularizationmentioning

confidence: 99%

Microfluidic Tissue Engineering and Bio‐Actuation

et al. 2022

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Bio‐hybrid technologies aim to replicate the unique capabilities of biological systems that could surpass advanced artificial technologies. Soft bio‐hybrid robots consist of synthetic and living materials and have the potential to self‐assemble, regenerate, work autonomously, and interact safely with other species and the environment. Cells require a sufficient exchange of nutrients and gases, which is guaranteed by convection and diffusive transport through liquid media. The functional development and long‐term survival of biological tissues in vitro can be improved by dynamic flow culture, but only microfluidic flow control can develop tissue with fine structuring and regulation at the microscale. Full control of tissue growth at the microscale will eventually lead to functional macroscale constructs, which are needed as the biological component of soft bio‐hybrid technologies. This review summarizes recent progress in microfluidic techniques to engineer biological tissues, focusing on the use of muscle cells for robotic bio‐actuation. Moreover, the instances in which bio‐actuation technologies greatly benefit from fusion with microfluidics are highlighted, which include: the microfabrication of matrices, biomimicry of cell microenvironments, tissue maturation, perfusion, and vascularization.

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Section: Tissue Vascularizationmentioning

confidence: 99%

Microfluidic Tissue Engineering and Bio‐Actuation

et al. 2022

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“…Recently, Salameh et al used 3D templating techniques to develop a vascularized FTSm that includes a more complex vasculature system than the previously described models [ 117 ]. The technique used to produce hollow channels inside the collagen matrix was similar to the work by Mori et al, using nylon wires [ 115 ].…”

Section: The Evolution Of Skin-on-a-chip Platformsmentioning

confidence: 99%

Skin-on-a-Chip Technology: Microengineering Physiologically Relevant In Vitro Skin Models

Zoio

Oliva

2022

Pharmaceutics

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The increased demand for physiologically relevant in vitro human skin models for testing pharmaceutical drugs has led to significant advancements in skin engineering. One of the most promising approaches is the use of in vitro microfluidic systems to generate advanced skin models, commonly known as skin-on-a-chip (SoC) devices. These devices allow the simulation of key mechanical, functional and structural features of the human skin, better mimicking the native microenvironment. Importantly, contrary to conventional cell culture techniques, SoC devices can perfuse the skin tissue, either by the inclusion of perfusable lumens or by the use of microfluidic channels acting as engineered vasculature. Moreover, integrating sensors on the SoC device allows real-time, non-destructive monitoring of skin function and the effect of topically and systemically applied drugs. In this Review, the major challenges and key prerequisites for the creation of physiologically relevant SoC devices for drug testing are considered. Technical (e.g., SoC fabrication and sensor integration) and biological (e.g., cell sourcing and scaffold materials) aspects are discussed. Recent advancements in SoC devices are here presented, and their main achievements and drawbacks are compared and discussed. Finally, this review highlights the current challenges that need to be overcome for the clinical translation of SoC devices.

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“…Finally, they proved that the system could be used as a tool for improving the skin-equivalents. More recently, Salameh et al 85 developed a vascularized skin equivalent with more complex blood vasculature, which represented a differentiated epidermis with physiological organization, three perfusable vascular channels, and a microvascular network connected to the macrovessels. They demonstrated that perfusion of the skin reconstructs and the presence of a complex vascular network could assess respective topical and systemic applications.…”

Section: Fabrication Approaches For Prevascularized Skinmentioning

confidence: 99%