A paraffin-celloidin embedding method for studying soft-hard tissue interfaces

Linder, J. E.; Longhurst, P; Johnson, Newell W.

doi:10.1016/0003-9969(81)90193-x

Cited by 6 publications

(2 citation statements)

References 5 publications

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“…After the second dehydration, the sample is left in a solution of paraffin wax and chloroform. Once the wax hardens, a smaller sample is sectioned on a microtome and mounted on a plastic slide (Linder et al, ). A stain is then applied and the section is ground following standard histological techniques (see Padian and Lamm, ).…”

Section: Methodsmentioning

confidence: 99%

The anatomy of the bifurcated neural spine and its occurence within Tetrapoda

Woodruff

2014

Journal of Morphology

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Vertebral neural spine bifurcation has been historically treated as largely restrictive to sauropodomorph dinosaurs; wherein it is inferred to be an adaptation in response to the increasing weight from the horizontally extended cervical column. Because no extant terrestrial vertebrates have massive, horizontally extended necks, extant forms with large cranial masses were examined for the presence of neural spine bifurcation. Here, I report for the first time on the soft tissue surrounding neural spine bifurcation in a terrestrial quadruped through the dissection of three Ankole-Watusi cattle. With horns weighing up to a combined 90 kg, the Ankole-Watusi is unlike any other breed of cattle in terms of cranial weight and presence of neural spine bifurcation. Using the Ankole-Watusi as a model, it appears that neural spine bifurcation plays a critical role in supporting a large mobile weight adjacent to the girdles. In addition to neural spine bifurcation being recognized within nonavian dinosaurs, this vertebral feature is also documented within many members of temnospondyls, captorhinids, seymouriamorphs, diadectomorphs, Aves, marsupials, artiodactyls, perissodactyls, and Primates, amongst others. This phylogenetic distribution indicates that spine bifurcation is more common than previously thought, and that this vertebral adaptation has contributed throughout the evolutionary history of tetrapods. Neural spine bifurcation should now be recognized as an anatomical component adapted by some vertebrates to deal with massive, horizontal, mobile weights adjacent the girdles.

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Section: Methodsmentioning

confidence: 99%

The anatomy of the bifurcated neural spine and its occurence within Tetrapoda

Woodruff

2014

Journal of Morphology

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“…Teeth and jaw bones with teeth are usually deealeified before making seetions for histologie analysis (Linder et al 1981). In some centers of dental research seetions of undeealeified bones or teeth are used for histologie study (Delling et al 1976, Schonfeld & Bossmann 1980.…”

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confidence: 99%

A method for the study of undecalcified bones and teeth with attached soft tissues*

Donath

Breuner

1982

J Oral Pathology Medicine

1,504

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A new sawing-grinding method is deseribed for the histologieal evaluation of jaw bones with teeth or bones eontaining implants (eeramie or metallie). The undeealeified bone is embedded in aerylie resin and sawed at 100 to 150 |im. The sliees are ground automatically toy a speeial maehine to a thickness of 5-10 |xm. The usually employed staining procedures for hard plastic embedded^'tissnes may be used. Plaque, fillings, erowns, bridges, implants and soft tissues are preserved in situ. Maeroscopic and microscopic detail of good quality is preserved for histological and morphometrical evaluation.Aeeepted for publiealioti 25 Deeember 1981

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