A method for the study of undecalcified bones and teeth with attached soft tissues*

Donath, K; Breuner, G.

doi:10.1111/j.1600-0714.1982.tb00172.x

Cited by 1,504 publications

(1,070 citation statements)

References 7 publications

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“…The three metaphyseal sections were proximal (A), medial (B), and distal (C). Each segment was embedded in a polymethylmethacrylate resin and a representative section (approximately 20 lm thick) was cut from each segment using a microcutting and grinding technique adapted from a technique described by Donath and Breuner [7]. The sections underwent paragon staining (a combination of basic fuchsin and toluidine blue) for qualitative histology and quantitative histomorphometry.…”

Section: Methodsmentioning

confidence: 99%

Bone Remodeling and Hydroxyapatite Resorption in Coated Primary Hip Prostheses

Tonino¹,

Wal²,

Heyligers³

et al. 2009

Clinical Orthopaedics &Amp; Related Research

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Hydroxyapatite coatings for THA promote bone ongrowth, but bone and coating are exposed to stress shielding-driven osteoclastic resorption. We asked: (1) if the resorption of hydroxyapatite coating and bone ongrowth correlated with demographics; (2) if the resorption related to the stem level; and (3) what happens to the implant-bone interface when all hydroxyapatite coating is resorbed? We recovered 13 femoral components from cadaveric specimens 3.3 to 11.2 years after uneventful primary THA. Three cross sections (proximal, medial, distal) of the hydroxyapatite-coated proximal implant sleeve were analyzed by measuring the percentage of residual hydroxyapatite and bone ongrowth on the implant perimeter. Hydroxyapatite resorption was independent of patient age but increased with time in vivo and mostly was gone after 8 years. Bone ongrowth was independent of time in vivo but decreased with aging patients. Only in the most proximal section did less residual hydroxyapatite correlate with less bone ongrowth. Hydroxyapatite resorption, which was more proximal than distal, showed no adverse effects on the implant-bone interface.

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Section: Methodsmentioning

confidence: 99%

Bone Remodeling and Hydroxyapatite Resorption in Coated Primary Hip Prostheses

Tonino¹,

Wal²,

Heyligers³

et al. 2009

Clinical Orthopaedics &Amp; Related Research

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“…Bone sections were made buccolingually, through the center of the longitudinal axis of a tooth root using a saw (Exakt 310 CP; Exakt Technologies, Oklahoma City, OK) under water lubrication. The undecalcified, unstained specimens were embedded in resin and then cut/ ground ( 80-100 mm thickness) using standard histological techniques (Donath and Breuner, 1982).…”

Section: Methodsmentioning

confidence: 99%

Remodeling dynamics in the alveolar process in skeletally mature dogs

et al. 2006

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Bone turnover rates can be altered by metabolic and mechanical demands. Due to the difference in the pattern of loading, we hypothesized that there are differences in bone remodeling rates between the maxillary and mandibular alveolar processes. Furthermore, in a canine model, the alveolar process of teeth that lack contact (e.g., second premolars) would have a different turnover rate than bone supporting teeth with functional contact (e.g., first molars). Six skeletally mature male dogs were given a pair of calcein labels. After sacrifice, specimens representing the anterior and posterior locations of both jaws were prepared for examination by histomorphometric methods to evaluate the bone volume/total volume (BV/ TV; %), bone volume (mm 2 ), mineral apposition rate (MAR; mm/day), and bone formation rate (BFR; %/year) in the alveolar process. There were no significant differences (P > 0.05) in the BV/TV within the jaws. The bone volume within the alveolar process of the mandible was 2.8-fold greater than in the maxilla. The MAR was not significantly different between the jaws and anteroposterior locations. However, the BFR was significantly (P < 0.0001) greater in the mandible than in the maxilla. The anterior location had higher (P ¼ 0.002) remodeling than the posterior location in the maxilla but not in the mandible. While there was a greater bone mass and increased remodeling in the mandible, no remodeling gradient in the coronal-apical direction was apparent in the alveolar process. Bone adaptation probably involves a complex interplay of bone turnover, mass, and architecture.

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“…An additional 10 hours elapsed until the polymerization process (blue light) was completed. We used three specimens from each patient to prepare ultrathin ground specimens of 25 lm thickness using an EXAKT machine (Norderstedt, Germany) [8]. After the polishing process, the specimens were stained with toluidine blue.…”

Section: Methodsmentioning

confidence: 99%

Articulating Spacers Used in Two-stage Revision of Infected Hip and Knee Prostheses Abrade with Time

Fink

Rechtenbach

Büchner

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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Background Articulating spacers used in two-stage revision surgery of infected prostheses have the potential to abrade and subsequently induce third-body wear of the new prosthesis. Questions/purposes We asked whether particulate material abraded from spacers could be detected in the synovial membrane 6 weeks after implantation when the spacers were removed for the second stage of the revision. Patients and Methods Sixteen hip spacers (cemented prosthesis stem articulating with a cement cup) and four knee spacers (customized mobile cement spacers) were explanted 6 weeks after implantation and the synovial membranes were removed at the same time. The membranes were examined by xray fluorescence spectroscopy, xray diffraction for the presence of abraded particles originating from the spacer material, and analyzed in a semiquantitative manner by inductively coupled plasma mass spectrometry. Histologic analyses also were performed.

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A method for the study of undecalcified bones and teeth with attached soft tissues*

Cited by 1,504 publications

References 7 publications

Bone Remodeling and Hydroxyapatite Resorption in Coated Primary Hip Prostheses

Bone Remodeling and Hydroxyapatite Resorption in Coated Primary Hip Prostheses

Remodeling dynamics in the alveolar process in skeletally mature dogs

Articulating Spacers Used in Two-stage Revision of Infected Hip and Knee Prostheses Abrade with Time

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