A Novel Water-Soluble Vitamin E Derivative Protects Against Experimental Colitis in Rats

Abstract

This study was designed to investigate the effects of water-soluble vitamin E derivative, 2-(alpha-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol (TMG), on experimental colitis in rats. Colitis was induced in male Wistar rats weighing 200 grams using an enema of trinitrobenzene sulfonic acid (TNBS) dissolved in 50% ethanol; 1 ml of TMG dissolved in physiological saline (0.2 mg/ml, 2 mg/ml, 20 mg/ml) was injected intraperitoneally every day for 1 week after the enema. The damage score, wet weight of the colon, and increase in body weight were estimated 1 week after the enema of TNBS. Thiobarbituric acid-reactive substances (TBA-RS), an index of lipid peroxidation, and the level of alpha-tocopherol or TMG in the colonic mucosa were measured 1 week after the induction of colitis. As a result, increase in body weight was inhibited by the induction of colitis, although the inhibition was reduced in the group treated with TMG. The damage score, wet weight and TBA-RS were increased significantly in the colitis group; however, they were inhibited by the administration of TMG. The alpha-tocopherol level in the colonic mucosa was reduced by the induction of colitis, wheres TMG could not be detected in the colonic mucosa of rats treated with TMG. These results suggest that TMG is effective for the treatment of colitis in rats induced by TNBS.

Effect of a Novel Water-Soluble Vitamin E Derivative, 2-(.ALPHA.-D-Glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol, on Dextran Sulfate Sodium-Induced Colitis in Mice

“…Polyunsaturated fatty acids of the cellular membranes are degraded by lipid peroxidation with subsequent disruption of membrane integrity, suggesting that lipid peroxidation mediated by oxygen radicals is an important cause of damage and destruction of cell membranes [32,33]. Our data support a previous finding in which colonic TBA-reactive substances were markedly reduced in TNBS-colitis mice treated with TMG [34], suggesting that induction of lipid peroxidation is an early and critical event in this experimental model of inflammatory bowel disease.…”

Molecular evidences on the benefit of N-acetylcysteine in experimental colitis

“…Some agents have already proved effective in experimental conditions, the antioxidants such as vitamin E, selenium, and trimetazinide were found to be beneficial in IBD [21,22]. Herbal products rich in antioxidants have also shown beneficial effects in IBD [8][9][10].…”

Plant originated glycoprotein has anti-oxidative and anti-inflammatory effects on dextran sulfate sodium-induced colitis in mouse

“…Once large numbers of neutrophils and macrophages are activated, these cells enter the injured mucosa of large intestine, leading to overproduction of oxygen free radicals and nitric oxide (NO) [4]. An increase of reactive oxidants in inflamed tissue can cause injury to target cells and also damage DNA, which could contribute to gene mutation and cancer development [5].…”