Plant originated glycoprotein has anti-oxidative and anti-inflammatory effects on dextran sulfate sodium-induced colitis in mouse

Abstract

SummaryWe investigated the preventive effect of glycoprotein (27 kDa) isolated from Gardenia jasminoides Ellis (GJE) fruits on colitis in dextran sulfate sodium (DSS, 3%)-induced A/J mice which were administrated orally for 7 days. Anti-inflammatory activity of GJE glycoprotein was assessed by neutrophil infiltration and colonic lipid peroxidation, and determined by myeloperoxidase (MPO) activity and levels of thiobarbituric acid reactive substances (TBARS), respectively in DSS treatment system. The activities of antioxidative enzymes [catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx)], activation of inflammation related mediators (iNOS, COX-2, and NF-jB), and production of nitric oxide (NO) and reactive oxygen species (ROS) were also measured. The results of this study showed that GJE glycoprotein (80lg/ml) has a scavenging property to inhibit the intracellular ROS production in RAW 264.7 cells and that GJE glycoprotein (80 mg/kg BW) significantly suppressed the increase in the MPO activity, TBARS level, and NO production, inflammation related mediators [iNOS, COX-2, and NF-jB (p50)] activity in DSS-induced mice. Interestingly, the activities of CAT, SOD, and GPx were gradually augmented after a supplement of GJE glycoprotein. Therefore, we suggest that GJE glycoprotein is preventive and therapeutic agent for the ulcerative colitis.

On the Use of Herbal Medicines in Management of Inflammatory Bowel Diseases: A Systematic Review of Animal and Human Studies

“…Different studies reported that curcumin from C. longa reduced Cox-2 level [22,32,36]. Another herbal therapy with this effect is G. jasminoides [38].…”

“…It was demonstrated that some herbal therapies can ameliorate IBD by their antioxidant activity. Zataria multiflora [29], curcumin from C. longa [22,31,32,47], Ziziphora clinopoides [33], Ginkgo biloba [35,37], essential oil of Satureja khuzestanica [34], glycoprotein from Gardenia jasminoides [38], ethanolic extract and nicotine from Nicotiana tabacum [42], and polysaccharide from Rheum tanguticum [61,62] were shown to reduce indicators of lipid peroxidation, including thiobarbituric acid reactive substances (TBARS), myeloperoxidase (MPO), and/or malondialdedyde (MDA) in animal models of IBD. Some of them, such asG.…”

“…Increased luminal and salivary NO have also been detected in IBD patients [59,76,77]. Herbal therapies such as a glucoside from Polygonum multiflorum [28], curcumin from C. longa [32], a glycoprotein from G. jasminoides [38], and theaflavin-3,3 0 -digallate from C. sinensis [40] have demonstrated their efficacy in IBD by reducing NO and iNOS levels ( Table 1).…”

The Vignette for V13N4 issue

“…However, the activities of catalase, superoxide dismutase and glutathione peroxidase were increased after a supplement of GJE glycoprotein. Present investigation suggests that GJE glycoprotein is a potential therapeutic agent for ulcerative colitis [28].…”

Phytochemistry, Pharmacological Activities and Intellectual Property Landscape of Gardenia jasminoides Ellis: a Review

“…neutrophil infiltration and colonic lipid peroxidation due to its scavenging property. 98 A novel anti-oxidant water-soluble polysaccharide was isolated from Gardenia jasminoides Ellis proved significant scavenging abilities. 99 …”