A novel tool for high‐resolution transmission electron microscopy of intact interfaces between bone and metallic implants

Engqvist, Håkan; Botton, Gianluigi A.; Couillard, Martin; Mohammadi, S.; Malmström, Johan; Emanuelsson, Lena; Hermansson, Leif; Phaneuf, Michael W.; Thomsen, Peter

doi:10.1002/jbm.a.30696

Cited by 55 publications

(43 citation statements)

References 10 publications

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“…Furthermore, the FIB sectioning was performed on undecalcified tissue with the implant left intact, thus minimizing introduction of artifacts due to the sectioning (Engqvist et al 2006). This contrasts with ultramicrotomy sectioning where the tissue either has to be decalcified (Listgarten et al 1992) and separated from the implant, or the bulk metal has to be removed leaving only the oxide layer (Sennerby et al 1991).…”

Section: Discussionmentioning

confidence: 99%

Forearm bone-anchored amputation prosthesis: A case study on the osseointegration

et al. 2008

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Background and purpose Bone-anchored titanium implants have been used for anchorage of amputation prostheses for more than one and a half decades. Histological and ultrastructural analyses were performed on a forearm amputation prosthesis after being in use for more than 11 years.Material, methods and results The implant was retrieved from the ulnar bone after a fatigue fracture of the titanium implant, and was clinically stable at the time of removal. The histological findings showed a large amount of bone within the threads and a high degree of apposition of mineralized bone to the implant surface. Ultrastructural analysis of thin samples prepared by focused ion-beam microscopy revealed an electron-dense layer at the interface and direct apposition of crystalline hydroxyapatite at the implant surface.Interpretation Our observations in this retrieval study provide a structural correlate to the functional properties and clinical results of amputation prostheses.

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Section: Discussionmentioning

confidence: 99%

Forearm bone-anchored amputation prosthesis: A case study on the osseointegration

et al. 2008

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“…However, there are only a small number of examples in the literature where these methods have been applied to biomineralization, including the study of mollusc sclerites (Suzuki et al, 2006) and shell (Valazquez-Castillo et al, 2006). FIB has also been applied to the study of interfaces between biological and innate surfaces in biomedical applications (Engqvist et al, 2006;Giannuzzi et al, 2007;Engqvist et al, 2008).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Characterization of biominerals in the radula teeth of the chiton, Acanthopleura hirtosa

Saunders

Kong

Shaw

et al. 2009

Journal of Structural Biology

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ABSTRACT:Understanding biomineralization processes provides a route to the formation of novel biomimetic materials with potential applications in fields from medicine to materials engineering. The teeth of chitons (marine molluscs) represent an excellent example of a composite biomineralized structure, comprising variable layers of iron oxide, iron oxyhydroxide and apatite. Previous studies of fully mineralized teeth using X-ray diffraction, Raman spectroscopy and scanning electron microscopy (SEM) have hinted at the underlying microstructure, but have lacked the resolution to provide vital information on fine scale structure, particularly at interfaces. While transmission electron microscopy (TEM) is capable of providing this information, difficulties in producing suitable samples from the hard, complex biocomposite have hindered progress. To overcome this problem we have used focused ion beam (FIB) processing to prepare precisely oriented sections across interfaces in fully mineralized teeth. In particular, the composite structure is found to be more complex than previously reported, with additional phases (goethite and amorphous apatite) and interface detail observed. This combination of FIB processing and TEM analysis has enabled us to investigate the structural and compositional properties of this complex biocomposite at higher resolution than previously reported and has the potential to significantly enhance future studies of biomineralisation in these animals.

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“…Sections were collected immediately on lacey carbon 300 mesh copper grids and dried for 1 hour at 37°C. Low-magnification bright-field imaging was performed using a JEOL 3010 microscope (JEOL, Peabody, MA) (27,28).…”

Section: Elastic Modulus Mappingmentioning

confidence: 99%

The aminobisphosphonate risedronate preserves localized mineral and material properties of bone in the presence of glucocorticoids

Balooch¹,

Yao²,

Ager³

et al. 2007

Arthritis & Rheumatism

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Objective. Glucocorticoids (GCs) alter bone strength such that patients receiving these medications have a high rate of fragility-related fractures. The purpose of this study was to assess whether concurrent treatment with GCs (prednisolone) and risedronate (an aminobisphosphonate) would prevent the reduction in bone strength induced by GCs, in a mouse model of GC-induced bone loss and in patients enrolled in a clinical study.Methods. We evaluated mice treated with prednisolone pellets alone, GCs plus risedronate, or placebo alone and iliac crest biopsy specimens obtained from patients who were treated with GCs plus placebo or GCs plus risedronate for 1 year. We measured the mass, architecture, and physical and material properties of bone (subject to therapeutic treatments) at nanoscale to macroscopic dimensions, using synchrotron x-ray tomography, elastic modulus mapping, transmission electron microscopy, and small-angle x-ray scattering techniques.Results. GC treatment reduced trabecular bone mass, microarchitecture, and the degree of bone mineralization and elastic modulus within the trabeculae. Concurrent treatment with GCs and risedronate prevented the deterioration of trabecular bone architecture, reduced the degree of mineralization, and preserved elastic modulus within the trabeculae, in both mouse and human bone. In addition, treatment with risedronate plus GCs in mice appeared to preserve bone crystal orientation, compared with treatment with GCs alone.Conclusion. Risedronate prevented the localized changes in mineral and material properties of bone induced by GCs, which may ultimately improve bone strength.

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A novel tool for high‐resolution transmission electron microscopy of intact interfaces between bone and metallic implants

Cited by 55 publications

References 10 publications

Forearm bone-anchored amputation prosthesis: A case study on the osseointegration

Forearm bone-anchored amputation prosthesis: A case study on the osseointegration

Characterization of biominerals in the radula teeth of the chiton, Acanthopleura hirtosa

The aminobisphosphonate risedronate preserves localized mineral and material properties of bone in the presence of glucocorticoids

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