A novel strategy with standardized reference extract qualification and single compound quantitative evaluation for quality control of Panax notoginseng used as a functional food

Li, S.P.; Qiao, Chun‐Feng; Chen, Y.W.; Zhao, Jing; Cui, Xiuming; Zhang, Q.W.; Liu, X.M.; Hu, De Jin

doi:10.1016/j.chroma.2013.07.025

Cited by 65 publications

(33 citation statements)

References 21 publications

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“…In most cases, herbal medicines produced their efficacies from the synergistic actions of multicomponents. During the last decades, an obvious trend is the switch from determination of single maker compound to multiple components [1][2][3][4]. A great number of methods have been developed to determine compounds as many as possible in herbal products [5].…”

Section: Introductionmentioning

confidence: 99%

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Design of ultraviolet wavelength and standard solution concentrations in relative response factors for simultaneous determination of multi-components with single reference standard in herbal medicines

Yang

Zhao

Fan

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 99%

“…It is frequently applied to determine multi-components to control the quality of complex botanical products by research institutions and pharmacopoeias [4,[7][8][9][10][11][12]. The method is also called simultaneous determination of multiple components (SDMC) or quantitative analysis of multi-components by single-marker (QAMS) [9,10].…”

Section: Introductionmentioning

confidence: 99%

Design of ultraviolet wavelength and standard solution concentrations in relative response factors for simultaneous determination of multi-components with single reference standard in herbal medicines

Yang

Zhao

Fan

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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“…Percent difference (PD) and Cos (θ), cosine similarity between two vectors, were employed. [16] The calculation of PD is: 100× (| x 1 − x 2 |)/[( x 1 + x 2 )/2]. The calculation of Cos (θ) is as the following equation:…”

Section: Methodsmentioning

confidence: 99%

Simultaneous determination of multiple ginsenosides in Panax ginseng herbal medicines with one single reference standard

Wu¹,

Guan²,

Wang³

et al. 2017

Phcog Mag

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Background:Root of Panax ginseng C. A. Mey (Renseng in Chinese) is a famous Traditional Chinese Medicine. Ginsenosides are the major bioactive components. However, the shortage and high cost of some ginsenoside reference standards make it is difficult for quality control of P. ginseng.Objective:A method, single standard for determination of multicomponents (SSDMC), was developed for the simultaneous determination of nine ginsenosides in P. ginseng (ginsenoside Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3, Rd).Materials and Methods:The analytes were separated on Inertsil ODS-3 C18 (250 mm × 4.6 mm, 5 μm) with gradient elution of acetonitrile and water. The flow rate was 1 mL/min and detection wavelength was set at 203 nm. The feasibility and accuracy of SSDMC were checked by the external standard method, and various high-performance liquid chromatographic (HPLC) instruments and chromatographic conditions were investigated to verify its applicability. Using ginsenoside Rg1 as the internal reference substance, the contents of other eight ginsenosides were calculated according to conversion factors (F) by HPLC.Results:The method was validated with linearity (r2 ≥ 0.9990), precision (relative standard deviation [RSD] ≤2.9%), accuracy (97.5%–100.8%, RSD ≤ 1.6%), repeatability, and stability. There was no significant difference between the SSDMC method and the external standard method.Conclusion:New SSDMC method could be considered as an ideal mean to analyze the components for which reference standards are not readily available.SUMMARY A method, single standard for determination of multicomponents (SSDMC), was established by high-performance liquid chromatography for the simultaneous determination of nine ginsenosides in Panax ginseng (ginsenoside Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3, Rd)Various chromatographic conditions were investigated to verify applicability of FsThe feasibility and accuracy of SSDMC were checked by the external standard method. Abbreviations used: DRT: Different value of retention time; F: Conversion factor; HPLC: High-performance Liquid Chromatography; LOD: Limit of detection; LOQ: Limit of quantitation; PD: Percent difference; PPD: 20(S)-protopanaxadiol; PPT: 20(S)-protopanaxatriol; RSD: Relative standard deviation; SSDMC: Single Standard for Determination of Multicomponents; TCM: Traditional Chinese Medicine.

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“…For these multi-symptoms, Traditional Chinese Medicine (TCM) becomes a preferred choice to meet the requirement of daily life and improve physiological and psychological well-being of patient [1,2]. In Eastern Asian countries including China, Japan and Korea, Fufang Ejiao Syrup (FES) has been reported with multiple beneficial functions on improving the sub-healthy state of human body, such as alleviating fatigue [3], ameliorating anemia [4] and boosting immunity [5].…”

Section: Introductionmentioning

confidence: 99%

Identification of bioactive ingredients with immuno-enhancement and anti-oxidative effects from Fufang-Ejiao-Syrup by LC–MS n combined with bioassays

Shen

Chen

Zhu

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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A novel strategy with standardized reference extract qualification and single compound quantitative evaluation for quality control of Panax notoginseng used as a functional food

Cited by 65 publications

References 21 publications

Design of ultraviolet wavelength and standard solution concentrations in relative response factors for simultaneous determination of multi-components with single reference standard in herbal medicines

Design of ultraviolet wavelength and standard solution concentrations in relative response factors for simultaneous determination of multi-components with single reference standard in herbal medicines

Simultaneous determination of multiple ginsenosides in Panax ginseng herbal medicines with one single reference standard

Identification of bioactive ingredients with immuno-enhancement and anti-oxidative effects from Fufang-Ejiao-Syrup by LC–MS n combined with bioassays

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