A novel strategy to immobilize enzymes on microporous membranes via dicarboxylic acid halides

Abstract

A simple and efficient enzyme immobilization strategy on microporous membrane surfaces using dicarboxylic acid halides as a spacer offers a tool to design membranes used in enzymatic membrane reactors.

A Modular Approach for Interlocking Enzymes in Whatman Paper

“…70 % activity was retained for Lacc‐PAA/Paper, and increased to 100 % when BSA was added, Lacc‐BSA‐PAA/Paper. BSA likely stabilizes laccase by preventing laccase self‐crosslinking in the second layer …”

Fabrication and Optimization of a Lipase Immobilized Enzymatic Membrane Bioreactor based on Polysulfone Gradient-Pore Hollow Fiber Membrane

“…Ulbricht emphasized that maintaining membrane characteristics should be the priority in order to prevent serious reduction on membrane performance (i.e., flux and selectivity) [32]. In this regard, functional groups such as carboxyl, hydroxyl and amine groups can be introduced onto the surface of polysulfone membranes through different surface modification methods without affecting the bulk properties and to covalently interact with lipases [8]. Ranieri et al prepared asymmetric ceramic hollow fiber membranes for covalent immobilization of lipase, and the lipase-immobilized membrane can be repeatedly used for six times without obvious activity loss.…”

“…Generally, EMBRs have the characteristics of elongating the lifetime of enzymes and improving their reusability, and the porous structure of membranes allows for a high specific surface area and relatively low diffusion resistance. Nevertheless, up to now, the usage of immobilized enzymes in industrial biocatalytic processes is still limited, and problems such as fabricating efficient enzyme-immobilized membranes and constructing controllable enzyme catalysis and separation systems remain to be solved in the research and practical application of EMBRs [7][8][9].…”

A Modular Approach for Interlocking Enzymes in Whatman Paper

“…Thee ffect of conjugation on enzymatic activity was calculated by measuring the initial linear increase in color (Supporting Information, Figure S6) normalized against the rate of unwashed laccase after syn-Scheme 1. [22] Thel ong-term stability of laccase at different storage temperatures is also of interest. Lacc-BSA/BSA-Paper showed enhanced enzymatic activity compared to unwashed laccase (ca.…”

“…70 %a ctivity was retained for Lacc-PAA/Paper,a nd increased to 100 %w hen BSA was added, Lacc-BSA-PAA/Paper.B SA likely stabilizes laccase by preventing laccase self-crosslinking in the second layer. [22] Thel ong-term stability of laccase at different storage temperatures is also of interest. Although immobilized laccase stability at 4 8 8Ci sreported, 25 8 8Cs tability is not well known ( Figure 3C).…”