A Modular Approach for Interlocking Enzymes in Whatman Paper

Riccardi, Caterina; McCormick, Shelby; Kasi, Rajeswari M.; Kumar, Challa V.

doi:10.1002/ange.201805074

Cited by 3 publications

(1 citation statement)

References 28 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…prolonged storage at noncontrolled temperature and humidity, no sophisticated lab infrastructures) given advantageous features in terms of ease of handling, costs, stability. In this direction, there is a high interest in developing new enabling strategies to expand the chemical space of celluloses and prime cellulosic surfaces for covalent and spatially resolved ligands bioconjugation [11][12][13][14][15][16] . Of particular interest, photo-initiated thiol-yne click chemistry has been recently introduced by the Levkin group to develop micro-patterned nanocellulosic substrates.…”

Section: Introductionmentioning

confidence: 99%

Clickable Cellulosic Surfaces for Peptide-Based Bioassays

Odinolfi

Romanato

Bergamaschi

et al. 2019

Preprint

View full text Add to dashboard Cite

The use of peptides in paper-based analytics is a highly appealing field, yet it suffers from severe limitations. This is mostly due to the loss of effective target recognition properties of this relatively small bioprobes upon nonspecific adsorption onto cellulose substrates. Here, we address this issue by introducing a simple polymer-based strategy to obtain clickable cellulosic surfaces, that we exploited for the chemoselective bioconjugation of peptide bioprobes. Our method largely outperformed standard adsorption-based immobilization strategy in a challenging, real-case immunoassay, namely the diagnostic discrimination of Zika+ individuals from healthy controls. Of note, the clickable polymeric coating not only allows efficient peptides bioconjugation, but it provides favorable anti-fouling properties to the cellulosic support. We envisage our strategy to broaden the repertoire of cellulosic materials manipulation and promote a renewed interest in peptide-based paper bioassays.

show abstract

Section: Introductionmentioning

confidence: 99%

Clickable Cellulosic Surfaces for Peptide-Based Bioassays

Odinolfi

Romanato

Bergamaschi

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

Biocatalytic Protein‐Based Materials for Integration into Energy Devices

et al. 2019

View full text Add to dashboard Cite

There is a current need to fabricate new biobased functional materials. Bottom‐up approaches to assemble simple molecular units have shown promise for biomaterial fabrication due to their tunability and versatility for the incorporation of functionalities. Herein, the fabrication of catalytic protein thin films by the entrapment of catalase into protein films composed of a scaffolding protein is demonstrated. Extensive structural and functional characterization of the films provide evidence of the structural integrity, order, stability, catalytic activity, and reusability of the biocatalytic materials. Finally, these functional biomaterials are coupled with piezoelectric disks to fabricate a second generation of bio‐inorganic generators. These devices are capable of producing electricity from renewable fuels through catalase‐driven gas production that mechanically stimulates the piezoelectric material.

show abstract