A novel strain of <i>Trichoderma viride</i> shows complete lignocellulolytic activities

Abstract

In this study we describe a novel dark-green strain of Trichoderma viride exhibiting complete ensemble of cellulase, hemicellulase and ligninase activities on specific plate assays. To assess the cellulase production in detail, basal salt medium (BSM) was fortified with synthetic (carboxymethyl cellulose (CMC), glucose, sucrose, dextrose, lactose or maltose) and natural (flours of banana, banana peel, jack seed, potato or tapioca) carbon as well as nitrogen (yeast extract, beef extract, peptone, NaNO 3 or NH 4 NO 3) sources. Temperature and pH optima were 28˚C and 4, respectively for the growth of the fungus in CMC-BSM with 137 U/mL cellulase activity, which was enhanced to 173 U/mL at 1.25% CMC concentration. Flours of potato and banana peel supported comparable yields of cellulase to that of CMC, while sodium nitrate was the preferred nitrogen source. The water soluble bluish-green pigment (a probable siderophore) extracted from the spores showed an absorption maximum at 292 nm. To sum up, the complete lignocellulolytic potential of this fungus offers great industrial significance, coupled with the production of a new pigment.

Lignocellulolytic activities of a novel strain of <i>Trichoderma harzianum</i>

“…The images of spores were also observed using phase contrast microscope (Figure 1(C)). The mycelia profusely branched with clustered fruiting bodies (phialides), however the phialides were not prominent as in T. viride [7]. Interestingly, though not frequent, the spherical sporogonia were seen attached on the mycelia along with frequent phialides.…”

“…Majority of the reports on commercial production of cellulases utilize submerged fermentation (SmF), because of its ease of controlling the conditions. However, in nature, the growth and cellulose utilization of aerobic microorganisms harbouring cellulases probably resemble solid-state fermentation (SSF) than a liquid culture [7]. Ten-fold reduction in production cost has been indicated by SSF than SmF [8].…”

Utilization of Stalks Waste of Sorghum bicolor (L.) Moench to Produce Bioethanol by using Saccharomyces cerevisiae and S. cerevisiae-Pichia stipitis Consortium

“…The degradation of sorghum stem occurred due to a combination of physical, acid and heating pretreatment and hydrolysis and fermentation enzymatically. T.viride is excellent in producing cellulase enzymes and effective in complementing the degradation of lignocellulose composition (Neethu et al, 2012). T. viride has the ability to degrade lignin during the hydrolysis process.…”

Optimization of Physical and Nutritional Factors for Enhanced Production of Lignocellulolytic Enzymes by Aspergillus terreus FJAT-31011 under Submerged Conditions

“…rice straw powder in the media, which showed the ability to utilize complex lignocellulosic materials in rice straw by producing oxidative and hydrolytic enzymes. Similarly, Neethu et al, (2012) fortified basal salt medium (BSM) with synthetic (carboxymethyl cellulose (CMC), glucose, sucrose, dextrose, lactose or maltose) and natural (flours of banana, banana peel, jack seed, potato or tapioca) carbon sources. They observed enhanced cellulase activity of 173 U/mL at 1.25% CMC concentration.…”