A novel silicon nitride biosensor for specific antibody–antigen interaction

Tlili, A.; Jarboui, Mohamed Ali; Abdelghani, Adnane; Fathallah, Dahmani M.; Maaref, M.A.

doi:10.1016/j.msec.2005.02.001

Cited by 50 publications

(30 citation statements)

References 24 publications

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“…11-Azido-1-undecanol was synthesized from 11-bromo-1-undecanol and sodium azide according to the literature. 81 4,4,5,5,6,6,7,7,8,8,9,9, was purchased from Sigma-Aldrich. Alexa Fluor 555 azide (9) and the fluorescent labeled lectin Alexa Fluor 647 conjugate of PNA from Arachis hypogaea (peanut) were supplied by Invitrogen.…”

Section: Methodsmentioning

confidence: 99%

“…4 Therefore, this ceramic is commonly deposited in the microelectronic industry as a passivation layer in, for instance, integrated circuits and solar cells. 5−7 On the other hand, being not only an insulator, but also a material with excellent biocompatibility, Si 3 N 4 has received considerable attention in biosensing 8,9 and medical applications. 10,11 Combined with silicon or silicon oxide, Si 3 N 4 is the preferred material for microcantilever-based biosensors, 12,13 for integrated optical waveguides, 14 and for functionalized AFM cantilevers.…”

Section: Introductionmentioning

confidence: 99%

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Copper-Free Click Biofunctionalization of Silicon Nitride Surfaces via Strain-Promoted Alkyne–Azide Cycloaddition Reactions

et al. 2012

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Cu-free "click" chemistry is explored on silicon nitride (Si(3)N(4)) surfaces as an effective way for oriented immobilization of biomolecules. An ω-unsaturated ester was grafted onto Si(3)N(4) using UV irradiation. Hydrolysis followed by carbodiimide-mediated activation yielded surface-bound active succinimidyl and pentafluorophenyl ester groups. These reactive surfaces were employed for the attachment of bicyclononyne with an amine spacer, which subsequently enabled room temperature strain-promoted azide-alkyne cycloaddition (SPAAC). This stepwise approach was characterized by means of static water contact angle, X-ray photoelectron spectroscopy, and fluorescence microscopy. The surface-bound SPAAC reaction was studied with both a fluorine-tagged azide and an azide-linked lactose, yielding hydrophobic and bioactive surfaces for which the presence of trace amounts of Cu ions would have been problematic. Additionally, patterning of the Si(3)N(4) surface using this metal-free click reaction with a fluorescent azide is shown. These results demonstrate the ability of the SPAAC as a generic tool for anchoring complex molecules onto a surface under extremely mild, namely ambient and metal-free, conditions in a clean and relatively fast manner.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Copper-Free Click Biofunctionalization of Silicon Nitride Surfaces via Strain-Promoted Alkyne–Azide Cycloaddition Reactions

et al. 2012

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“…Surface plasmon resonance (SPR) biosensors have made great strides both in terms of technology and applications [16] such as characterizing and quantifying biomolecular interactions [17][18][19], determination of affinity and binding constants [20][21][22], monitoring [23,24], diagnosis [25][26][27][28] and DNA sensing [29,30].…”

Section: Introductionmentioning

confidence: 99%

4-Aminophenyl boronic acid modified gold platforms for influenza diagnosis

Di̇ltemi̇z

Ersöz

Hür

et al. 2013

Materials Science and Engineering: C

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“…The proposed methods are mostly limited to the functionalization of the native SiO 2 layer present on the Si 3 N 4 film (Diao et al, 2005;Tlili et al, 2005;Wu et al, 2006). In general, silane chemistries are extremely sensitive to pH, temperature and humidity, which means that these methods are unreliable given the inconsistent and variable functionalization yield across the modified surface, which results in uncoated areas.…”

Section: Introductionmentioning

confidence: 99%