A novel semi-multiplex PCR assay for identification of tropical eels of genus Anguilla in Indonesian waters

Fahmi, Melta Rini; Solihin, Dedy Duryadi; Soewardi, Kadarwan; Pouyaud, Laurent; Shao, Zhipeng; Berrebi, Patrick

doi:10.1007/s12562-012-0587-0

Cited by 12 publications

(8 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…So a rapid and efficient identification technique is needed. Semimultiplex PCR developed in the present study has successfully distinguished seven species that inhabit the waters of Indonesia through one-step PCR (Fahmi et al 2012). The semi-multiplex method proposed in this study has demonstrated the efficiency for identifying seven species and sub-species of tropical eels with only one step PCR.…”

mentioning

confidence: 82%

Conservation genetic of tropical eel in Indonesian waters based on population genetic study

Fahmi¹

2015

Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia

Self Cite

View full text Add to dashboard Cite

Ikan sidat tropis (Anguilla spp.) dari Indonesia saat ini menjadi komoditas yang penting di pasar internasional dimikian juga penelitian ikan sidat tropis saat ini menjadi sebuah tantangan baru. Salah satu permasalah utama pada ikan sidat tropis adalah terjadinya tumpang tindih antara karakter morfologi spesies, sehingga identifikasi spesies dengan menggunakan pendekatan morfologi tidak bisa dilakukan. sehingga identifikasi spesies ikan sidat dilakukan dengan pendekatan molekuler. Metode Semi-multiplek PCR yang dikembangkan pada penelitian ini mampu mengidentifikasi tujuh spesies dan subspecies ikan sidatan yang mendiami perairan Indonesia hanya dengan menggunakan satu step PCR. Berdasarkan identifikasi dengan menggunakan semi-multiplek PRC diketahui ada empat spesies ikan sidat yang memiliki penyebaran luas yaitu Anguilla bicolor bicolor, A. b. pacifica, A. marmorata dan A. interioris, dua spesies memiliki penyebaran yang sempit dan mendekati endemis: A. celebesensis dan A. borneensis dan spesies A. nebulosa nebulosa yang hanya ditemukan di perairan barat Indonesia yaitu sungai yang berhubungan dengan Samudera Hindia. Berdasarkan analisis DNA mitokondria gen cytochrome b, tujuh spesies yang mendiami perairan Indonesia memperlihatkan keragaman haplotipe dan nucleotide yang tinggi yaitu masing-masing 0.98 dan 4.57%. Analisa kekerabatan menunjukkan A. borneensis merupakan nenek moyang ikan sidat tropis di perairan Indonesia.

show abstract

mentioning

confidence: 82%

Conservation genetic of tropical eel in Indonesian waters based on population genetic study

Fahmi¹

2015

Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia

Self Cite

View full text Add to dashboard Cite

show abstract

“…Itoi et al (2005) also reported a method to distinguish the commercially distributed species of A. japonica and A. anguilla using specific probes for each species. In addition, to identify seven species/subspecies of Anguilla in Indonesian waters, Fahmi et al (2013) used PCR primers specific to each species/subspecies. Batch analysis based on the species-specific PCR primer to verify the presence of A. japonica has also been recently reported (Suzuki et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Genetic identification method for two subspecies of the Indonesian short-finned eel, Anguilla bicolor, using an allelic discrimination technique

et al. 2014

View full text Add to dashboard Cite

Background: There is an increasing pressure on tropical species of the freshwater eel genus Anguilla for use in aquaculture, so species identification methods are needed to monitor these activities and to facilitate conservation efforts. A method was developed to genetically distinguish two subspecies of the Indonesian short-finned eel, Anguilla bicolor, based on an allelic discrimination technique. Results: A single DNA nucleotide substitution in the mitochondrial DNA 16S rRNA gene was employed to identify the two subspecies where Anguilla bicolor bicolor and Anguilla bicolor pacifica possessed adenine and guanine, respectively. This substitution was highly conserved at 100% in A. bicolor bicolor (108/108) and 99.9% in A. bicolor pacifica (181/182), and the misidentification rate was estimated to be 0.34%. Subsequently, fluorescent-labeled oligo probes and PCR primers were designed and succeeded to clearly distinguish the two subspecies. Further, the other ten anguillid species that may be sympatrically distributed with A. bicolor showed negative results. Conclusions: The method developed in this study is useful to accurately identify the two subspecies of A. bicolor and can contribute to ecological studies, stock management, and conservation.

show abstract

“…Three species taken from the Dumoga estuary with ADV numbers in the range of 6-12 are important from biodiversity and biogeography perspective in order to identify the species that actually exist. Identification of glass eels based on DNA from one or more recent species has been developed by Fahmi et al, (2013). Seasonal and annual variability needs to be observed in the recruitment.…”

Section: Methods Of Researchmentioning

confidence: 99%

Type Composition of Eel Seed (Anguilla Spp.) in Dumoga Creek, Bolaang Mongondow

Kusuma¹,

Herawati²,

Sambah³

2018

RJOAS

View full text Add to dashboard Cite

Dumoga River is one of the rivers in Bolaang Mongondow Regency disembogue to the Sulawesi Sea which becomes the entrance of glass eel from the sea. Glass eel that enters the river consists of several species. The purpose of this study was to study the species composition of glass eel that migrates to the creek of the Dumoga River. Glass eels were collected at the creek from 20th of April 2018, to 30th of May 2018 at 00:00 to 04:00. In a period of 40 days, there were 2,251 glass eels collected and consist of 3 species namely Anguilla marmorata (49.13%), Anguilla bicolor pacifica (37.94%), and Anguilla spp. (12.93%). Glass eels are stored in formalin for morphometric and meristic measurements. Morphometric was done by calculating the AD value (DA = 100 (LD-LA) LT-1) and the tail pigmentation structure. Meristic was done by calculating the eyelid spine. The result showed that the value of AD obtained ranged from (0-2.87) grouped in the type of bicolor pacifica, (14.90% -16.14%), grouped in the type A. marmorata and (6.85% 7.21%) grouped in the Anguilla sp.

show abstract

A novel semi-multiplex PCR assay for identification of tropical eels of genus Anguilla in Indonesian waters

Cited by 12 publications

References 23 publications

Conservation genetic of tropical eel in Indonesian waters based on population genetic study

Conservation genetic of tropical eel in Indonesian waters based on population genetic study

Genetic identification method for two subspecies of the Indonesian short-finned eel, Anguilla bicolor, using an allelic discrimination technique

Type Composition of Eel Seed (Anguilla Spp.) in Dumoga Creek, Bolaang Mongondow

Contact Info

Product

Resources

About