A novel ratiometric fluorescent probe for the detection of mitochondrial pH dynamics during cell damage

Fu, Gaoqing; Yin, Guoxing; Niu, Tingting; Wu, Wei; Han, Hui; Chen, Haimin; Yin, Peng

doi:10.1039/d0an01240h

Cited by 15 publications

(5 citation statements)

References 29 publications

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“…The nanoparticles were modified with azide groups and chelated Co 2+ to prepare the Click-XRN Co with an average particle size of 119.9 nm, which had enough localization accuracy for micrometer-sized mitochondria. As a mitochondria-targeting agent, TPP has demonstrated the capacity to effectively integrate diverse functional molecules and produce delocalized positive charges, affording it the capability to traverse the hydrophobic double-layer membrane of mitochondria. − At present, as lipophilic cations, TPP has been widely used in the development of fluorogenic probes for detecting mitochondrial biomarkers, demonstrating its efficacy in specifically targeting mitochondria. ,, …”

Section: Resultsmentioning

confidence: 99%

“…32−35 At present, as lipophilic cations, TPP has been widely used in the development of fluorogenic probes for detecting mitochondrial biomarkers, demonstrating its efficacy in specifically targeting mitochondria. 32,36,37 We selected HeLa cells and incubated them with TPP-PEGalkyne to achieve modification of the mitochondria through alkyne groups. Subsequently, Click-XRN Co was employed for the recognition and labeling of mitochondria (Figure 3a).…”

Section: Cell Labeling and Click-xrmmentioning

confidence: 99%

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Clickable X-ray Nanoprobes for Nanoscopic Bioimaging of Cellular Structures

Tang,

Yin,

Liu

et al. 2024

JACS Au

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Synchrotron-based X-ray microscopy (XRM) has garnered widespread attention from researchers due to its high spatial resolution and excellent energy (element) resolution. Existing molecular probes suitable for XRM include immune probes and genetic labeling probes, enabling the precise imaging of various biological targets within cells. However, immune labeling techniques are prone to cross-interference between antigens and antibodies. Genetic labeling technologies have limited systems that allow express markers independently, and moreover, genetically encoded labels based on catalytic polymerization lack a fixed morphology. When applied to cell imaging, this can result in reduced localization accuracy due to the diffusion of labels within the cells. Therefore, both techniques face challenges in simultaneously labeling multiple biotargets within cells and achieving high-precision imaging. In this work, we applied the click reaction and developed a third category of imaging probes suitable for XRM, termed clickable X-ray nanoprobes (Click-XRN). Click-XRN consists of two components: an X-ray-sensitive multicolor imaging module and a particle-size-controllable morphology module. Efficient identification of intra-and extracellular biotargets is achieved through click reactions between the probe and biomolecules. Click-XRN possesses a controllable particle size, and its loading of various metal ions provides distinctive signals for imaging under XRM. Based on this, we optimized the imaging energy of Click-XRN with different particle sizes, enabling single-color and two-color imaging of the cell membrane, cell nucleus, and mitochondria with nanoscale spatial nanometers. Our work provides a potent molecular tool for investigating cellular activities through XRM.

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Section: Resultsmentioning

confidence: 99%

Section: Cell Labeling and Click-xrmmentioning

confidence: 99%

Clickable X-ray Nanoprobes for Nanoscopic Bioimaging of Cellular Structures

Tang,

Yin,

Liu

et al. 2024

JACS Au

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“…3c). 97 Although it was applied for the fluorescence imaging of live cells and in vivo zebrafish models, its emission signal changes in the pH range of 8.5 to 11 ( λ ex = 470 nm), which is not promising for further exploration as a suitable probe for bio-imaging at the cellular pH. Similarly, Tang and co-workers synthesized probe 17 by conjugating a benzothiazole phenol derivative with a hemicyanine group (Fig.…”

Section: Ratiometric Fluorescent Probes For Ph Mapping In Organellesmentioning

confidence: 99%

Ratiometric fluorescent probes for pH mapping in cellular organelles

Munan¹,

Yadav²,

Pareek³

et al. 2023

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“…All these TPP + -modified nanovehicles exhibit mitochondrial targeting ability and excellent anti-tumor effect in vivo and in vitro . In addition, TPP + has been widely used in the design of fluorogenic probes for the detection of mitochondrial biomarkers ( Wang et al, 2014 ; Fu et al, 2021 ) ( Figure 3A ). By conjugating TPP + , a NIR reporter, and a sulfenic acid-reactive group, Gao et al synthesized a novel NIR probe DATC, which is able to visualize endogenous protein sulfenic acids expressed in the mitochondria ( Gao et al, 2020 ).…”

Section: Mitochondria-targeting Agentsmentioning

confidence: 99%

Recent Advances in Chemical Biology of Mitochondria Targeting

et al. 2021

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Mitochondria are vital subcellular organelles that generate most cellular chemical energy, regulate cell metabolism and maintain cell function. Mitochondrial dysfunction is directly linked to numerous diseases including neurodegenerative disorders, diabetes, thyroid squamous disease, cancer and septicemia. Thus, the design of specific mitochondria-targeting molecules and the realization of real-time acquisition of mitochondrial activity are powerful tools in the study and treatment of mitochondria dysfunction in related diseases. Recent advances in mitochondria-targeting agents have led to several important mitochondria chemical probes that offer the opportunity for selective targeting molecules, novel biological applications and therapeutic strategies. This review details the structural and physiological functional characteristics of mitochondria, and comprehensively summarizes and classifies mitochondria-targeting agents. In addition, their pros and cons and their related chemical biological applications are discussed. Finally, the potential biomedical applications of these agents are briefly prospected.

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A novel ratiometric fluorescent probe for the detection of mitochondrial pH dynamics during cell damage

Abstract: A sensitive fluorescent probe (E)-4-(3-(benzo[d]thiazol-2-yl)-4-hydroxy-5-methylstyryl)-1-methylpyridin-1-ium iodide (HBTMP) for the monitoring of pH in mitochondria was rationally exploited.

Cited by 15 publications

References 29 publications

Clickable X-ray Nanoprobes for Nanoscopic Bioimaging of Cellular Structures

Clickable X-ray Nanoprobes for Nanoscopic Bioimaging of Cellular Structures

Ratiometric fluorescent probes for pH mapping in cellular organelles

Recent Advances in Chemical Biology of Mitochondria Targeting

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