A Novel Protein with Alkaline Phosphatase and Protease Inhibitor Activities in Streptomyces hiroshimensis.

Nitta, Masayuki; Goto, Mariko; Shibuya, Naomi; Okawa, Yoshio

doi:10.1248/bpb.25.833

Cited by 9 publications

(3 citation statements)

References 20 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results we obtained represent confirmation of previously reported data related to conditions for synthesis of proteinase inhibitors by various Streptomycetes.…”

Section: Discussionsupporting

confidence: 91%

Screening for production of proteinase inhibitors by Antarctic Streptomycetes

Dimitrova-Stefanova

Gocheva

2018

J Basic Microbiol

View full text Add to dashboard Cite

Three out of 17 Streptomycetes strains – Streptomyces sp. 35 LBG09, Streptomyces sp. 36 LBG09, and Streptomyces sp. 39 LBG09, were selected based on the high production of proteinase inhibitors with trypsin serine proteinase activity. The strains were isolated from soil samples taken from the area around the Bulgarian station on Livingston Island, Antarctica. Biosynthesis of proteinase inhibitors by the promising strains started at different stages of their development but was generally not associated with the growth of the producers. Peak levels were reached in the stationary phase (96–120 h) of their cultivation. Inducing effects on strain development, biomass accumulation, and proteinase inhibitor biosynthesis were based on the composition of the nutrient medium: the polypeptones contained in Taguchi medium and glucose as a carbon source. The most productive out of the three strains was Streptomyces sp. 36 LBG09. Its maximum inhibitory activity was reached at 96 h in culturing media modified by three different carbon sources. The active proteinase inhibitor biosynthesis proceeded at pH values between 6.8 and 8.6 and the dynamics of production depended on the type of carbon source. Peak levels of extracellular protein and dry biomass were reached at 120 h in the stationary growth phase. The residual sugars were minimal at the end of the process when using soluble starch as a carbon source, and maximal when glucose was used.

show abstract

“…The results we obtained represent confirmation of previously reported data related to conditions for synthesis of proteinase inhibitors by various Streptomycetes.…”

Section: Discussionsupporting

confidence: 91%

Screening for production of proteinase inhibitors by Antarctic Streptomycetes

Dimitrova-Stefanova

Gocheva

2018

J Basic Microbiol

View full text Add to dashboard Cite

show abstract

“…Bakteri pelarut fosfat bukan merupakan satusatunya kelompok bakteri yang dapat melarutkan P yang terabsorpsi permukaan oksida-oksida besi dan alumunium sebagai senyawa Fe-P dan Al-P (Hartono 2000). Beberapa kelompok Actinomycetes (Nitta et al, 2002) dan kelompok jamur (Aleksieva et al, 2003), dapat juga melarutkan Fe-P dan Al-P.…”

Section: Pendahuluanunclassified

APLIKASI PUPUK ORGANIK DAN BAKTERI PELARUT FOSFAT UNTUK MENINGKATKAN PERTUMBUHAN DAN HASIL TANAMAN JAGUNG MANIS MANIS (Zea mays saccaharta sturt L.)

Maulana,

Irmawatie

2022

j.ilmutanaman, j.tanaman, j.agronomi

View full text Add to dashboard Cite

The use of organic matters on sweet corn cultivation is focused to decrease the utilization of chemical substances for minimizing environmental degradation. An experiment was conducted at Ujung Berung Indah Residence, Ujung Berung Districts, Bandung City (± 780 m asl.) from April to July 2020. The Aims of this research were to determine the suitable and effective inoculant to increase the growth of sweet corn plants. The sweet corn variety used was Bonanza F1 and the PSB inoculant that used was Bacillus Laterosporus. The organic fertilizers were compost, chicken and cow dung, and phosphate solubilizing bacteria (PSB), as a biofertilizer. A randomized block design with three replications was utilized in this experiment. The treatments consisted of without organic fertilizers or PSB (control) (K), inoculation of PSB (P1), application of compost (P2), (4) application of chicken and cow dung (P3), and application of the chemical fertilizer (NPK) (P4). The results showed that the application of organic fertilizers and PSB increased the growth of sweet corn plants after harvesting. The inoculation of PSB resulted in highest plant height compared to control and the highest sweet corn yield compared to the control treatment, and even it was higher than the yield caused by the application of the chemical fertilizer.

show abstract

“…Besides, large amounts of alkaline phosphatase enzyme are reported to be secreted by Streptomyces species. AlP enzymes were purified from some species, such as from S. griseus, S. hygroscopicus and S. hiroshimensis [70][71][72], although these enzymes are not closely related to the phoD of Bacillus subtilis. The lack of a significant relationship between AlP activity and the phoD copy number in our study (Table S4) might be associated with the presence of various genes coding for alkaline phosphatase enzymes.…”

Section: Potential Psb Strainsmentioning

confidence: 99%

Metagenomic Analysis of Bacterial Communities in Agricultural Soils from Vietnam with Special Attention to Phosphate Solubilizing Bacteria

2021

View full text Add to dashboard Cite

Bacterial communities can promote increased phosphorus (P) availability for plants and microbes in soil via various mechanisms of phosphate solubilization. The production of extracellular phosphatases releases available P through the hydrolysis of organic P. Examining the abundance and diversity of the bacterial community, including phosphate solubilizing bacteria in soil, may provide valuable information to overcome P scarcity in soil ecosystems. Here, the diversity and relative abundance of bacterial phyla and genera of six agricultural soil samples from Vietnam were analysed by next generation sequencing of the 16S rRNA gene. Phosphatase activities of each soil were compared with physico-chemical parameters and the abundance of the alkaline phosphatase gene phoD. We showed the dominance of Chloroflexi, Proteobacteria, Actinobacteria, Acidobacteria and Firmicutes. Total nitrogen positively correlated with phyla Proteobacteria, Acidobacteria, Firmicutes and Planctomycetes. The abundance of several genera of Proteobacteria showed positive relationship with the copy number of the phoD gene. The abundance of several taxa positively correlated with silt content, while a negative relationship of Proteobacteria was found with sand content. Our results demonstrated the clear influence of soil physico-chemical properties on the abundance of various bacterial taxa including those potentially involved in phosphate solubilization.

show abstract

A Novel Protein with Alkaline Phosphatase and Protease Inhibitor Activities in Streptomyces hiroshimensis.

Cited by 9 publications

References 20 publications

Screening for production of proteinase inhibitors by Antarctic Streptomycetes

Screening for production of proteinase inhibitors by Antarctic Streptomycetes

APLIKASI PUPUK ORGANIK DAN BAKTERI PELARUT FOSFAT UNTUK MENINGKATKAN PERTUMBUHAN DAN HASIL TANAMAN JAGUNG MANIS MANIS (Zea mays saccaharta sturt L.)

Metagenomic Analysis of Bacterial Communities in Agricultural Soils from Vietnam with Special Attention to Phosphate Solubilizing Bacteria

Contact Info

Product

Resources

About