A Novel Multiplex–Protein Array for Serum Diagnostics of Colorectal Cancer: Impact of Pre-analytical Storage Conditions

Bünger, Stefanie; Klempt-Giessing, Katja; Toner, Vicki; Kelly, Maria D.; Fitzgerald, S.; Brenner, Hermann; Eggeling, Ferdinand von; Habermann, Jens K.

doi:10.1089/bio.2013.0050

Cited by 7 publications

(9 citation statements)

References 40 publications

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“…Contrarily, VEGF-R2 was slightly but significantly increased after three freeze/thaw cycles (168). Similar results were obtained by Bünger and colleagues who used a biochipbased measurement in biobanked sera from colorectal cancer patients (170).…”

Section: Pre-analytical Factorssupporting

confidence: 82%

Measuring myokines with cardiovascular functions: pre-analytical variables affecting the analytical output

Lombardi¹,

Sansoni²,

Banfi³

2017

Ann. Transl. Med.

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Abstract:In the last few years, a growing number of molecules have been associated to an endocrine function of the skeletal muscle. Circulating myokine levels, in turn, have been associated with several pathophysiological conditions including the cardiovascular ones. However, data from different studies are often not completely comparable or even discordant. This would be due, at least in part, to the whole set of situations related to the preparation of the patient prior to blood sampling, blood sampling procedure, processing and/or store. This entire process constitutes the pre-analytical phase. The importance of the preanalytical phase is often not considered. However, in routine diagnostics, the 70% of the errors are in this phase. Moreover, errors during the pre-analytical phase are carried over in the analytical phase and affects the final output. In research, for example, when samples are collected over a long time and by different laboratories, a standardized procedure for sample collecting and the correct procedure for sample storage are acknowledged. In this review, we discuss the pre-analytical variables potentially affecting the measurement of myokines with cardiovascular functions.

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Section: Pre-analytical Factorssupporting

confidence: 82%

Measuring myokines with cardiovascular functions: pre-analytical variables affecting the analytical output

Lombardi¹,

Sansoni²,

Banfi³

2017

Ann. Transl. Med.

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“…After exclusion for reasons, such as reviews, basic research papers, non CRC or IL-8 topic, totally 46 articles were included for full text review, and in the review, 28 articles were excluded for reasons(list of full-text excluded articles and reasons for exclusion could be seen in S1 Appendix ). Finally, a total of 18 eligible studies were included in the final meta-analysis[ 3 , 8 , 12 , 22 – 36 ]. Of these 18 publications, 15 articles evaluated the association between IL-8 expression and clinicopathologic features or prognosis of colorectal cancer, and 5 were for diagnostic evaluation.…”

Section: Resultsmentioning

confidence: 99%

Prognostic Value, Clinicopathologic Features and Diagnostic Accuracy of Interleukin-8 in Colorectal Cancer: A Meta-Analysis

et al. 2015

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BackgroundThe prognostic value and diagnostic accuracy of Interleukin-8 (IL-8) in colorectal cancer have been assessed with several studies, but the conclusions were inconclusive. Thus we performed a meta-analysis to evaluate the impact of IL-8 expression on colorectal cancer prognosis, clinicopathologic features and diagnostic accuracy.MethodsComprehensive search strategies were used to search relevant literature in the PubMed, EBSCO and the ISI Web of Science databases. The correlation between IL-8 expression and prognosis, clinicopathologic features and diagnostic accuracy was analyzed.ResultsA total of 18 articles met the inclusion criteria, including 1509 patients for clinicopathologic features or prognosis evaluation and 725 participants for diagnostic evaluation. The results suggested that overexpression of IL-8 was significantly associated with poor prognosis in colorectal cancer (HR = 1.54, 95%CI 1.03–2.32), especially in Union for International Cancer Control (UICC) stage IV patients (HR = 2.28, 95%CI 1.60–3.25). With further subgroup analysis, we found that high IL-8 level in serum was significantly correlated with poor prognosis (HR = 2.13, 95%CI 1.49–3.05). In addition, significant correlations were observed between high IL-8 expression and advanced stage (OR = 3.01, 95%CI 1.98–4.56), lymphatic metastasis (OR = 2.24, 95%CI 1.39–3.63), and liver metastasis (OR = 3.47, 95%CI 1.74–6.89). Moreover, IL-8 had high diagnostic accuracy, with pooled sensitivity 0.70(95%CI 0.66–0.74), specificity 0.91(95%CI 0.86–0.94), positive likelihood ratio (LR) 7.00(95%CI 2.48–19.73), negative LR 0.24(95%CI 0.09–0.64), diagnostic OR 24.00(95%CI 5.52–104.38).ConclusionsThis study showed that IL-8 could be a potential indicator for detecting colorectal cancer and predicting prognosis. In addition, high IL-8 level was significantly correlated with advanced stage, lymphatic metastasis, liver metastasis.

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“…In the context of medical research, it has been proven that different storage temperatures and temperature fluctuations during storage impact sample integrity with regard to biomarkers of interest. 30,[43][44][45][46] The impact of storage conditions has also been demonstrated in cases of individual HBMrelevant substances, for example, polychlorinated biphenyls, brominated flame retardants, some pesticides, or arsenic species. 36,47 It is therefore reasonable to assume that other environmental pollutants might be equally affected by different storage conditions.…”

Section: Discussionmentioning

confidence: 99%

Towards Harmonized Biobanking for Biomonitoring: A Comparison of Human Biomonitoring-Related and Clinical Biorepositories

Lermen

Gwinner

Bartel-Steinbach

et al. 2020

Biopreservation and Biobanking

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Human biomonitoring (HBM) depends on high-quality human samples to identify status and trends in exposure and ensure comparability of results. In this context, much effort has been put into the development of standardized processes and quality assurance for sampling and chemical analysis, while effects of sample storage and shipment on sample quality have been less thoroughly addressed. To characterize the currently applied storage and shipment procedures within the consortium of the European Human Biomonitoring Initiative (HBM4EU), which aims at harmonization of HBM in Europe, a requirement analysis based on data from an online survey was conducted. In addition, the online survey was addressed to professionals in clinical biobanking represented by members of the European, Middle Eastern and African Society for Biopreservation and Biobanking (ESBB) to identify the current state-of-the-art in terms of sample storage and shipment. Results of this survey conducted in these two networks were compared to detect processes with potential for optimization and harmonization. In general, many similarities exist in sample storage and shipment procedures applied by ESBB members and HBM4EU partners and many requirements for ensuring sample quality are already met also by HBM4EU partners. Nevertheless, a need for improvement was identified for individual steps in sample storage, shipment, and related data management with potential impact on sample and data quality for HBM purposes. Based on these findings, recommendations for crucial first steps to further strengthen sample quality, and thus foster advancement in HBM on a pan-European level are given.

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A Novel Multiplex–Protein Array for Serum Diagnostics of Colorectal Cancer: Impact of Pre-analytical Storage Conditions

Cited by 7 publications

References 40 publications

Measuring myokines with cardiovascular functions: pre-analytical variables affecting the analytical output

Measuring myokines with cardiovascular functions: pre-analytical variables affecting the analytical output

Prognostic Value, Clinicopathologic Features and Diagnostic Accuracy of Interleukin-8 in Colorectal Cancer: A Meta-Analysis

Towards Harmonized Biobanking for Biomonitoring: A Comparison of Human Biomonitoring-Related and Clinical Biorepositories

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