A Novel Human Prostate-Specific, Androgen-Regulated Homeobox Gene (NKX3.1) That Maps to 8p21, a Region Frequently Deleted in Prostate Cancer

He, Wei; Sciavolino, Peter J.; Wing, John P.; Augustus, Meena; Hudson, Peter; Meissner, P S; Curtis, R T; Shell, Brenda K.; Bostwick, David G.; Tindall, Donald J.; Gelmann, Edward P.; Abate‐Shen, Cory; Carter, K C

doi:10.1006/geno.1997.4715

Cited by 326 publications

(251 citation statements)

References 26 publications

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“…NKX3.1 proved to be the only gene, which was down-regulated in expression in all treatment groups. These observations contrast results of others [43], who were able to show that NKX3.1 expression is up-regulated upon androgen treatment of LNCaP cells. Tetramethrin treatment for 6 h showed the weakest effect on gene expression.…”

Section: Discussioncontrasting

confidence: 99%

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Characterisation of gene expression patterns in 22RV1 cells for determination of environmental androgenic/antiandrogenic compounds

Hartel

Didier

Pfaffl

et al. 2003

The Journal of Steroid Biochemistry and Molecular Biology

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Alteration of androgen receptor function due to hormonally active compounds in the environment, may be responsible for impaired reproductive function in aquatic wildlife. Based on human prostate carcinoma 22RV1 cells, a cell culture expression system was established to test effects of putative androgenic/antiandrogenic compounds on endogenous gene expression. 22RV1 cells were shown to express human androgen receptor, but not human progestin (hPR) or human oestrogen receptor (hER) ␣ and ␤. Six androgen-regulated genes (ARGs) were chosen to determine androgenic/antiandrogenic action using highly sensitive real-time RT-PCR. Results showed that gene expression is altered in a time-dependent manner. After stimulation of cells by DHT (10 nM), synthetic androgen R1881 (1 nM), or organic pesticides (difenoconazole, fentinacetate, tetramethrin) TMPRSS2 mRNA expression was down-regulated by the factor 0.6 after 24 h of DHT treatment. Similar results were obtained when cells were assayed for mRNA expression of PSA after fentinacetate and R1881 stimulation. In contrast, TMPRSS2 expression was up-regulated by the factor 0.9 when cells were stimulated by tetramethrin. Final goal of the work is a sensitive determination of differential gene expression by different compounds under study, achievement of substance-specific expression patterns and function related analysis of potential androgens/antiandrogens.

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Section: Discussioncontrasting

confidence: 99%

“…M99487) is a cell surface marker in the prostate [42], homeobox gene NKX3.1 (accession no. U860669) [43] is involved in cell growth and differentiation, TMPRSS2 (accession no. AF270487) supports normal cell growth and morphology [44], androgen receptor (AR accession no.…”

Section: Introductionmentioning

confidence: 99%

Characterisation of gene expression patterns in 22RV1 cells for determination of environmental androgenic/antiandrogenic compounds

Hartel

Didier

Pfaffl

et al. 2003

The Journal of Steroid Biochemistry and Molecular Biology

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“…Chromosomal region 8p22 contains the known putative TSGs MSR1 and N33. 22,23 He et al 24 mapped the NKX3.1 gene to 8p21, which is a human prostate-specific, androgen-regulated homeobox gene. Many studies indicate that loss of 8p12-21 is an early event in prostate carcinogenesis, whereas loss of 8p22 is a later event that is common in advanced prostate cancers.…”

Section: Chromosomal Lossesmentioning

confidence: 99%

Genetic changes in pT2 and pT3 prostate cancer detected by comparative genomic hybridization

Fukasawa

Kino

Kobayashi

et al. 2007

Prostate Cancer Prostatic Dis

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Prostate-specific antigen (PSA) screening has led to a remarkable increase in prostate cancer cases undergoing operative therapy. Over half of patients with locally advanced cancer (^pT3) develop rising PSA levels (biochemical failure) within 10 years. It is very difficult to predict which patients will progress rapidly to advanced disease following biochemical failure (BF). Therefore, a more useful prognostic factor is needed to suggest the most appropriate therapies for each patient. To determine chromosomal aberrations, we examined 30 patients with stage pT2 or pT3 primary prostate adenocarcinomas and no metastases (pN0M0) by comparative genomic hybridization (CGH). Laser capture microdissection (LCM) was used to gather cancer cells from frozen prostate specimens. Common chromosomal alterations included losses on 2q23-24, 4q26-28, 6q14-22, 8p12-22 and 13q21-31, as well as gains on 1p32-36, 6p21 and 17q21-22. Losses at 8p12-22 and 13q21-31 were observed more frequently in pT3 than pT2 tumors (Po0.05 and Po0.01, respectively). Losses at 8p12-22 were more frequent in tumors with BF (Po0.05), and those at 13q12-21 were more frequent in tumors with Gleason score (GS) 7 or more than lower GS (Po0.05). These findings suggest that losses of 8p12-22 and 13q21-31 are important determinants of prostate cancer progression.

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“…In Europe, United States and other developed countries, it is the second common malignant tumor in males (Daskivich et al, 2011;Jemal et al, 2011). In prostate cancer patients, the common gene changes include loss of NKX3.1 (8p21) and PTEN (10q23), increased androgen receptor (AR), and fusion of ETS family transcription factors genes with androgen promoter (He et al, 1997;Li et al, 1997;Nguyen and Wang, 2008). A recent study of 112 cases of prostate cancer revealed that MED12 mutations…”

Section: Med12 Mutation and Prostate Cancermentioning

confidence: 99%

MED12 mutations in human diseases

Wang

Qin²,

et al. 2013

Protein Cell

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A Novel Human Prostate-Specific, Androgen-Regulated Homeobox Gene (NKX3.1) That Maps to 8p21, a Region Frequently Deleted in Prostate Cancer

Cited by 326 publications

References 26 publications

Characterisation of gene expression patterns in 22RV1 cells for determination of environmental androgenic/antiandrogenic compounds

Characterisation of gene expression patterns in 22RV1 cells for determination of environmental androgenic/antiandrogenic compounds

Genetic changes in pT2 and pT3 prostate cancer detected by comparative genomic hybridization

MED12 mutations in human diseases

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