A novel HBV genotypes detecting system combined with microfluidic chip, loop-mediated isothermal amplification and GMR sensors

Zhi, Xiao; Deng, Min; Yang, Hao; Gao, Guo; Wang, Kan; Fu, Hualin; Zhang, Yixia; Chen, Di; Cui, Daxiang

doi:10.1016/j.bios.2013.11.025

Cited by 77 publications

(44 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4b Optical- and electrochemical-based detection modalities are the most frequently used mechanisms in developing LAMP-based microchip platforms. However, novel detection techniques such as those based on giant magneto-resistance (GMR) 98 and bioluminescence 16a have also been developed for the detection and monitoring of LAMP-amplified biotargets. LAMP-LFT-based POC assays seem to have significant potential for commercialization due to its low cost and ease of use by nonskilled personnel.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Emerging Loop-Mediated Isothermal Amplification-Based Microchip and Microdevice Technologies for Nucleic Acid Detection

Safavieh

Kanakasabapathy

Tarlan

et al. 2016

ACS Biomater. Sci. Eng.

151

104

View full text Add to dashboard Cite

Rapid, sensitive, and selective pathogen detection is of paramount importance in infectious disease diagnosis and treatment monitoring. Currently available diagnostic assays based on polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) are time-consuming, complex, and relatively expensive, thus limiting their utility in resource-limited settings. Loop-mediated isothermal amplification (LAMP) technique has been used extensively in the development of rapid and sensitive diagnostic assays for pathogen detection and nucleic acid analysis and hold great promise for revolutionizing point-of-care molecular diagnostics. Here, we review novel LAMP-based lab-on-a-chip (LOC) diagnostic assays developed for pathogen detection over the past several years. We review various LOC platforms based on their design strategies for pathogen detection and discuss LAMP-based platforms still in development and already in the commercial pipeline. This review is intended as a guide to the use of LAMP techniques in LOC platforms for molecular diagnostics and genomic amplifications.

show abstract

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Emerging Loop-Mediated Isothermal Amplification-Based Microchip and Microdevice Technologies for Nucleic Acid Detection

Safavieh

Kanakasabapathy

Tarlan

et al. 2016

ACS Biomater. Sci. Eng.

151

104

View full text Add to dashboard Cite

show abstract

“…Recent studies have successfully utilized both antibodies and DNA-probes as sensing material, achieving extremely sensitive detection with LODs in the zeptomolar range. Zhi et al (2014) showed detection of Hepatitis B virus (HBV)-genotype in a microfluidic system capable of identifying as few as 10 copies/ml. Furthermore, Lian et al (2012) showed a fully realized lab-on-a-chip solution ( Figure 10) for diagnosing acute myocardial infarction.…”

Section: Magnetoresistive Sensorsmentioning

confidence: 99%

Recent advances in lab-on-a-chip for biosensing applications

Lafleur

Jonsson

Senkbeil

et al. 2016

Biosensors and Bioelectronics

207

106

View full text Add to dashboard Cite

“…Although research in magnetoresistance biosensors is at an early stage it can be envisaged that the technique will prove a powerful technique in the field of nanosensors. In one format, magnetoresistance sensors exploit the changes in magnetism of paramagnetic beads that provides a more selective and sensitive (lower detection limit of 10 pg/l) label compared to traditional fluorescent labels (Zhi et al, 2014).…”

Section: Magnetoresistance Sensorsmentioning

confidence: 99%

“…Yet, a continuous flow PCR microfluidic chip coupled with hybridization enable detection of multiple pathogens in an automated device (Jiang et al, 2014). With advances in isothermal PCR techniques that does not require thermal cycling it can be envisaged that major advances in pathogen detection and is currently under development (Zhi et al, 2014).…”

Section: Microfluidic (Lab-on-a-chip) Sensorsmentioning

confidence: 99%

Developments in nanoparticles for use in biosensors to assess food safety and quality

Warriner

Reddy

Namvar

et al. 2014

Trends in Food Science & Technology

View full text Add to dashboard Cite

Highlights•Background to the evolution of biosensor based on nanoparticles.•Application of nanoparticles in biosensor devices.•Overview of biosensor formats applied in food testing.•Examples of integrated biosensor systems for food sampling.•Prospects and challenges in nanosensors in pathogen testing.The following will provide an overview on how advances in nanoparticle technology have contributed towards developing biosensors to screen for safety and quality markers associated with foods. The novel properties of nanoparticles will be described and how such characteristics have been exploited in sensor design will be provided. All the biosensor formats were initially developed for the health care sector to meet the demand for point-of-care diagnostics. As a consequence, research has been directed towards miniaturization thereby reducing the sample volume to nanolitres. However, the needs of the food sector are very different which may ultimately limit commercial application of nanoparticle based nanosensors. IntroductionThrough evolution we have developed senses to enable us to differentiate good foods from bad. The art of cheese making, brewing and viticulture has relied on the producer using their senses to assess quality. Establishing if a food was safe was more problematic and typically relied on tasters to ensure dishes provided to their masters were sound. Clearly, analytical testing is a more reliable and objective means of assessing the quality and safety of foods. Laboratory testing provided a basis for developing protocols and limits that could be applied to foods to define quality or safety. Yet, the expense and delay in acquiring results remain critical limitations. Consequently, there is an on-going demand for biosensors that can be used outside the laboratory environment to assess the safety and quality of foods on-site.In many instances the needs of the food industry mirrored that of the health sector where rapid diagnostics would enhance treatments and prognosis. The health sector provides a more lucrative market for diagnostic companies so it is not unexpected to find that the majority of research to date has been towards developing biosensors to meet the market demands. Consequently, those biosensors encountered in the food sector are derived from those devices initially fabricated to meet the needs of the health care sector. Within this context, the following review provides a brief history on how the field of biosensors developed and the contribution made by advances in nanoparticle technology. The efforts in transferring biosensor technologies to the food sector will be provided with a focus on safety, and to a degree, quality assessment. The review does not attempt to provide an exhaustive list of advances given the numerous quality reviews are already published in this area (Arugula and Simonian,

show abstract

A novel HBV genotypes detecting system combined with microfluidic chip, loop-mediated isothermal amplification and GMR sensors

Cited by 77 publications

References 68 publications

Emerging Loop-Mediated Isothermal Amplification-Based Microchip and Microdevice Technologies for Nucleic Acid Detection

Emerging Loop-Mediated Isothermal Amplification-Based Microchip and Microdevice Technologies for Nucleic Acid Detection

Recent advances in lab-on-a-chip for biosensing applications

Developments in nanoparticles for use in biosensors to assess food safety and quality

Contact Info

Product

Resources

About