A novel Gossypium barbadense ERF transcription factor, GbERFb, regulation host response and resistance to Verticillium dahliae in tobacco

Liu, Jianguang; Wang, Yongqiang; Zhao, Guiyuan; Zhao, Junli; Du, Honglin; He, Xiaoliang

doi:10.1007/s12298-016-0402-y

Cited by 25 publications

(16 citation statements)

References 43 publications

(50 reference statements)

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“…ET often synergizes with JA and increases plant tolerance to necrotrophic pathogens (Zhu et al, 2011). ET is a small gaseous hormone involved in plant innate immunity against pathogens, and various studies have revealed that ET plays a role in resistance to V. dahliae in tobacco and cotton (Yang et al, 2015;Guo et al, 2016;Liu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

GhWRKY70D13 Regulates Resistance to Verticillium dahliae in Cotton Through the Ethylene and Jasmonic Acid Signaling Pathways

et al. 2020

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Verticillium wilt caused by Verticillium dahliae is a destructive cotton disease causing severe yield and quality losses worldwide. WRKY transcription factors play important roles in plant defense against pathogen infection. However, little has been reported on the functions of WRKYs in cotton's resistance to V. dahliae. Here, we identified 5, 5, and 10 WRKY70 genes in Gossypium arboreum, Gossypium raimondii, and Gossypium hirsutum, respectively, and investigated the expression profiles of all GhWRKY70 genes in various cotton tissues and in response to hormone treatment or V. dahliae infection. Reverse transcription-quantitative PCR analysis showed that GhWRKY70D13 was expressed higher in roots and stems than in other tissues, and up-regulated after V. dahliae inoculation. Knock-down of GhWRKY70D13 improved resistance to V. dahliae in both resistant and susceptible cotton cultivars. Comparative analysis of transcriptomes generated from wild-type and stable RNAi (RNA interference) plant with down-regulated GhWRKY70D13 showed that genes involved in ethylene (ET) and jasmonic acid (JA) biosynthesis and signaling were significantly upregulated in the GhWRKY70D13 RNAi plants. Consistently, the contents of 1-aminocyclopropane-1-carboxylic (ACC), JA, and JA-isoleucine levels were significantly higher in the GhWRKY70D13 RNAi plants than in wild-type. Following V. dahliae infection, the levels of ACC and JA decreased in the GhWRKY70D13 RNAi plants but still significantly higher (for ACC) than that in wild-type or at the same level (for JA) as in non-infected wild-type plants. Collectively, our results suggested that GhWRKY70D13 negatively regulates cotton's resistance to V. dahliae mainly through its effect on ET and JA biosynthesis and signaling pathways.

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Section: Introductionmentioning

confidence: 99%

GhWRKY70D13 Regulates Resistance to Verticillium dahliae in Cotton Through the Ethylene and Jasmonic Acid Signaling Pathways

et al. 2020

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“…48 The transcription factors of the WRKY, AP2/ERF, MYC, bZIP, and MYB families are involved in the regulation of defense gene expression when attacked by pathogens. 49 , 50 MYC transcription factors are important regulators of JA-responsive genes, negatively regulating the expression of the defense genes in Arabidopsis . 49 , 51 ERF is a transcription factor of the AP2/ERF subfamily and is involved in the defense responses of plants to pathogenic bacteria via the SA and Ethylene (ET)/JA-dependent signal transduction pathways.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome Sequencing Analysis Provides Insights Into the Response to Fusarium oxysporum in Lilium pumilum

Zhang

et al. 2019

Evol Bioinform Online

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Lily basal rot, caused by Fusarium oxysporum f. sp. lilii , is one of the most serious diseases of lily. Although the lily germplasm which is resistant to F. oxysporum has been used in disease-resistant breeding, few studies on its molecular mechanism of disease resistance have been reported. To comprehensively study the mechanism of resistance to F. oxysporum , transcriptome sequencings of root tissues from Lilium pumilum inoculated with F. oxysporum or sterile water for 6, 12, or 24 h were performed. A total of 50 GB of data were obtained from the transcriptome sequencings of the 6 L. pumilum samples, and 217 098 Unigenes were obtained after the de novo assembly, of which 38.36% Unigenes were annotated. The sequencing results showed that the numbers of differentially expressed genes at 6, 12, and 24 h after inoculation compared with the control were 111, 254, and 2500, respectively. The functional enrichment analysis of the differentially expressed genes showed that several pathways were involved in responses of L. pumilum , mainly including starch and sucrose metabolism, glycolysis/gluconeogenesis, phenylpropanoid biosynthesis, plant hormone signal transduction, flavonoid biosynthesis, vitamin B6 (VB6) biosynthesis, acid biosynthesis, proteasome, and ribosome. Transcription factor analysis revealed that the WRKY and ERF families played important roles in responses of L. pumilum to F. oxysporum . The results of this study elucidate the molecular responses to F. oxysporum in lily and lay a theoretical foundation for improving lily breeding and strategies for lily basal rot resistance.

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“…It has been reported that plenty of TFs like bHLH [49][50], MYB [51][52], ERF [53][54][55], NAC [56][57], WRKY [58][59][60] were related to various plant resistance against pathogens. In our study, the bHLH transcription factor family was the most abundant transcription factor in the interaction between sesame and MP.…”

Section: Defense-related Tfsmentioning

confidence: 99%

The Mechanism of Sesame Resistance Against Macrophomina Phaseolina Was Revealed via Comparison of Transcriptomes of Resistant and Susceptible Sesame Genotypes

Yan

Liu

et al. 2020

Preprint

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Background: Sesame (sesamum indicum) charcoal rot, a destructive fungal disease caused by Macrophomina phaseolina (Tassi) Goid (MP), is a great threat to the yield and quality of sesame. However, there is a lack of information revealing gene-for-gene relationship between sesame and MP, and the molecular mechanism in the interaction is not yet clear. The aim of this study is to interpret the molecular mechanism of sesame resistance against MP in disease-resistant (DR) and disease-susceptible (DS) genotypes based on transcriptomes, which is the first report on the interaction between sesame and MP from the transcriptomes.Results: A set of core genes that function in sesame disease resistance were revealed in our investigation by comparative transcriptomics, which preferentially associate with GO terms such as ribosome-related processes, fruit ripening, regulation of jasmonic acid mediated signaling pathway and cell-cell signaling. In addition, 52 potential resistance-related genes differentially and persistently expressed all through were obtained in sesame. Furthermore, we preliminarily studied the immune response mechanism of sesame against MP and found that auxin signal pathway shows negative regulation in the process. Also, the most highlighted pathway JA/ET signal transduction plays a negative regulatory role in sesame resistance against MP. Finally, a JAZ gene (LOC105168467) and an ERF transcription factor (LOC105171710) that may be related to disease resistance were screened. Conclusions: In our investigation, the mechanism of resistance against MP from the transcriptomes were illustrated, which is a complex bioprocess involved in many phytohormones and disease resistance-related genes. The findings not only shed more light on mechanism of interaction between sesame and MP, but also help us identify key genes and transcription factors (TFs) associated with charcoal rot resistance in sesame.

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A novel Gossypium barbadense ERF transcription factor, GbERFb, regulation host response and resistance to Verticillium dahliae in tobacco

Cited by 25 publications

References 43 publications

GhWRKY70D13 Regulates Resistance to Verticillium dahliae in Cotton Through the Ethylene and Jasmonic Acid Signaling Pathways

GhWRKY70D13 Regulates Resistance to Verticillium dahliae in Cotton Through the Ethylene and Jasmonic Acid Signaling Pathways

Transcriptome Sequencing Analysis Provides Insights Into the Response to Fusarium oxysporum in Lilium pumilum

The Mechanism of Sesame Resistance Against Macrophomina Phaseolina Was Revealed via Comparison of Transcriptomes of Resistant and Susceptible Sesame Genotypes

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