Transcriptome Sequencing Analysis Provides Insights Into the Response to Fusarium oxysporum in Lilium pumilum

Abstract

Lily basal rot, caused by Fusarium oxysporum f. sp. lilii , is one of the most serious diseases of lily. Although the lily germplasm which is resistant to F. oxysporum has been used in disease-resistant breeding, few studies on its molecular mechanism of disease resistance have been reported. To comprehensively study the mechanism of resistance to F. oxysporum , transcriptome sequencings of root tissues from Lilium pumilum inoculated with F. oxysporum or sterile water for 6, 12, or 24 h were performed. A total of 50 GB of data were obtained from the transcriptome sequencings of the 6 L. pumilum samples, and 217 098 Unigenes were obtained after the de novo assembly, of which 38.36% Unigenes were annotated. The sequencing results showed that the numbers of differentially expressed genes at 6, 12, and 24 h after inoculation compared with the control were 111, 254, and 2500, respectively. The functional enrichment analysis of the differentially expressed genes showed that several pathways were involved in responses of L. pumilum , mainly including starch and sucrose metabolism, glycolysis/gluconeogenesis, phenylpropanoid biosynthesis, plant hormone signal transduction, flavonoid biosynthesis, vitamin B6 (VB6) biosynthesis, acid biosynthesis, proteasome, and ribosome. Transcription factor analysis revealed that the WRKY and ERF families played important roles in responses of L. pumilum to F. oxysporum . The results of this study elucidate the molecular responses to F. oxysporum in lily and lay a theoretical foundation for improving lily breeding and strategies for lily basal rot resistance.

Crosstalk of DNA Methylation Triggered by Pathogen in Poplars With Different Resistances

“…Moreover, Vitamin B6 contributed to disease resistance against Pseudomonas syringae and Botrytis cinerea in Arabidopsis ( Zhang et al, 2015 ; Chandrasekaran et al, 2019 ). Transcriptome sequencing indicated that the VitB6 biosynthesis pathway was involved in the response of Lilium pumilum to Fusarium oxysporum ( He et al, 2019 ). Two protein families, PDX1s and PDX2, were required for the de novo biosynthesis of VitB6 ( Tambasco-Studart et al, 2007 ).…”

Genomics assisted functional characterization of Bacillus velezensis E as a biocontrol and growth promoting bacterium for lily

“…Lily basal rot and lily gray mold are important diseases that endanger lily production ( Cui et al, 2018 ; He et al, 2019 ). Although biological control has been widely used to control many plant diseases, the biological control of lily diseases has not been extensively studied.…”

“…In recent years, diseases affecting lily have become increasingly serious because of continuous cropping. Lily basal rot (also known as root rot or stem rot) and lily gray mold have seriously affected the yield and quality of lily, and resulting in huge economic losses ( Cui et al, 2018 ; He et al, 2019 ). Lily basal rot is a soil-borne disease that mainly affects the underground parts of plants and results in wilting, yellowing, and rotting of the bulbs.…”

“…Currently, chemical control methods are employed to control lily basal rot and lily gray mold. However, chemical control has shown dwindling effects because the pathogens are seed-borne or soil-borne, and pathogens develop resistance to the chemicals used ( Harold et al, 1997 ; He et al, 2019 ). Simultaneously, the long-term, large-scale and repeated use of chemical pesticides has caused environmental pollution and safety problems in edible and medicinal lily.…”

Lilium regale Wilson WRKY2 Regulates Chitinase Gene Expression During the Response to the Root Rot Pathogen Fusarium oxysporum

“…Root rot caused by Fusarium oxysporum seriously threatens the growth and development of lily ( Lilium spp.). Several wild Lilium species are highly resistant to F. oxysporum , including L. regale Wilson, L. pumilum , and L. dauricum Ker Gawler (He et al, 2014 , 2019 ; Ramekar et al, 2019 ). Although the germplasm resources of Lilium species resistant to F. oxysporum have been used for breeding disease-resistant lines, little is known about their mechanisms regulating transcription during an infection by F. oxysporum .…”