A novel fluorometric turn-on assay for lipase activity based on an aggregation-induced emission (AIE) luminogen

Abstract

Lipase plays critical roles in the industrial catalysis, drug discovery and medical diagnosis of diseases. In this paper, based on the consideration that a tetraphenylethylene (TPE) derivative P1 with a-COOC 6 H 13 group can be hydrolyzed into TPE-COOH which shows low solubility in same solutions and thus aggregation can occur easily, a novel fluorescence turn-on probe bearing aggregation-induced emission (AIE) characteristics to rapidly visualize lipase activities has been constructed with compound P1. Lipase of concentrations as low as 0.1 mg mL-1 can be detected and the assay range of lipase was located in 0.1-1.3 mg mL-1. On the other hand, the selectivity of probe P1, dynamic monitoring of enzymatic reaction and preliminary commercially enzyme activity screening was also summarized.

Fluorometric probing of the lipase level as acute pancreatitis biomarkers based on interfacially controlled aggregation-induced emission (AIE)

“…As mentioned above, in view of the fact that lipase catalytic reactions only occurred at the oil–water interface, compared to the reported probe P1 , 38 herein, carboxyl and amino groups that will improve the affinity of the probe towards the lipase in the aqueous phase were specifically introduced into probe S1 . Firstly, the substrate dependence of the initial degradation velocities for lipase were measured (Fig.…”

“… 20 – 37 Nevertheless, fluorescent probes for lipase level detection was a virgin area until our work was published. 38 In our previous work, an aggregation-induced emission (AIE)-based fluorescence probe bearing characteristics to rapidly visualize the lipase activities was constructed with compound P1 . Unfortunately, however, the sensitivity and detection limit were quite poor and the range of detection was outside the concentration range of the lipase level in human serum.…”

Activity‐based smart AIEgens for detection, bioimaging, and therapeutics: Recent progress and outlook

“…In the presence of lipase, the ‐COOC 6 H 13 unit was rapidly cleaved, which prompted transformation from the probe to the relatively insoluble enzymatic product in the same solution. [ 217 ] The self‐assembled aggregates subsequently formed with “turn‐on” AIE fluorescence. There was an excellent liner relationship between fluorescence intensities and various lipase concentrations (0.1‐1.3 mg/mL) .…”

Analyte-responsive fluorescent probes with AIE characteristic based on the change of covalent bond

“…In their subsequent work [48], a patent fluorescent light-up bioprobe was constructed with a limit of detection of 0.1 mg mL −1 for lipase activity based on TPE derivative P1 with an ester group which can be activated. And Liu's group [49] utilized a cleavable aminoacrylate linker which responded to singlet oxygen to conjugate a fluorescent green emissive rhodol dye and a red AIEbased photosensitizer to realize real-time monitoring of ROS generation during photodynamic therapy.…”