A novel fluorescent probe for ratiometric detection of formaldehyde in real food samples, living tissues and zebrafish

Yuan, Gonglin; Ding, Haiyuan; Peng, Longpeng; Zhou, Liyi; Lin, Qinlu

doi:10.1016/j.foodchem.2020.127221

Cited by 42 publications

(10 citation statements)

References 33 publications

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“…Genetically encoded fluorescent proteins (FPs)-based Förster resonance energy transfer (FRET) technology has been widely used for mapping temporal-spatial dynamics of intracellular biochemical events in living cells [1][2][3][4][5][6][7][8][9][10][11] "3-cube FRET" microscopy, referred to 3-cube FRET method is the most extensively applied approach for live-cell FRET quantification. [12][13][14][15] Three images are needed for E-FRET image: I DD (DA), image from donor channel with donor excitation; I AA (DA), image from acceptor channel with acceptor excitation; and I DA (DA), image from acceptor channel with donor excitation.…”

Section: Introductionmentioning

confidence: 99%

Measuring calibration factors by imaging a dish of cells expressing different tandem constructs plasmids

et al. 2021

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Three-cube Förster resonance energy transfer (FRET) method is the most extensively applied approach for live-cell FRET quantification. Reliable measurements of calibration factors are crucial for quantitative FRET measurement. We here proposed a modified TA-G method (termed as mTA-G) to simultaneously obtain the FRETsensitized quenching transition factor (G) and extinction coefficients ratio (γ) between donor and acceptor. mTA-G method includes four steps: ( 1) predetermining the ratio ranges of the sensitized emission of acceptor (F C ) to the donor excitation and donor channel image (I DD [(DA])) for all FRET plasmids; (2) culturing the cells which express every FRET plasmid in one dish respectively; (3) distinguishing and marking the cells expressing different FRET plasmids by detecting their F C /I DD (DA) values; (4) linearly fitting F C /I AA (DA) (acceptor excitation and acceptor channel image) to I DD (DA)/I AA (DA)for different kinds of cells. We implemented mTA-G method by imaging tandem constructs cells with different FRET efficiency cultured in one dish on different days, and obtained consistent G and γ values. mTA-G method not only circumvents switchover of different culture dishes but also keep the constant imaging conditions, exhibiting excellent robustness, and thus will expands the biological applications of quantitative FRET analysis in living cells.

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Section: Introductionmentioning

confidence: 99%

Measuring calibration factors by imaging a dish of cells expressing different tandem constructs plasmids

et al. 2021

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“…89 In order to detect the levels of FA in food, Lin and Bao et al designed naphthalimide-based probes (66,67) that could be applied to the detection of FA in real food, such as dried fish, frozen shrimp, frozen small octopus, frozen chicken, bananas squid and fresh octopus with high recoveries. 90,91 Besides, probes 66 and 67 were able to image FA in cells and living zebrafish (Fig. 14).…”

Section: Methylenehydrazine-based Mechanismmentioning

confidence: 99%

Reaction-based fluorescent and chemiluminescent probes for formaldehyde detection and imaging

Huang

Liu

et al. 2022

Chem. Commun.

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“…Fluorescence resonance energy transfer (FRET) has been proven to be a feasible mechanism for the design of hydrazine‐based ratiometric probes. However, only rare successful paradigms have been validated because of the high energy match requirement of the FRET mechanism [34,35] . Therefore, developing hydrazine‐based ratiometric probes remains a challenge.…”

Section: Introductionmentioning

confidence: 99%

“…However, only rare successful paradigms have been validated because of the high energy match requirement of the FRET mechanism. [34,35] Therefore, developing hydrazinebased ratiometric probes remains a challenge.…”

Section: Introductionmentioning

confidence: 99%

A Ratiometric Fluorescent Nanoprobe for Ultrafast Detection of Formaldehyde in Wood and Food Samples

et al. 2023

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Fluorescent sensing of formaldehyde (FA) in the environment is of great significance for human health. However, the development of ratiometric fluorescent probes for ultrasensitive FA detection is challenging due to the lack of suitable strategies. In this study, we demonstrate that ratiometric FA nanoprobes can be facile constructed by integrating two simultaneously excitable fluorophores into a polymer matrix. Using this strategy, we developed a ratiometric fluorescent nanoprobe (Nano‐FAP‐1) for the highly sensitive detection of FA in environmental samples by incorporating a 4‐hydrazino‐1,8‐naphthalimide derivative (Na‐FA) as the receptor and 7‐diethylaminocoumarin‐3‐carboxyl ethyl acetate (Cou‐1) as the reference into a Pluronic F‐127 matrix. The probe achieved a highly sensitive and selective response to low concentrations of FA, with a response time of fewer than 5 s and a detection limit of 0.407 μM. It is worth noting that the probe can be used for the determination of FA in wood and food samples, demonstrating its potential application in the field of toxic gas monitoring and environmental protection.

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A novel fluorescent probe for ratiometric detection of formaldehyde in real food samples, living tissues and zebrafish

Cited by 42 publications

References 33 publications

Measuring calibration factors by imaging a dish of cells expressing different tandem constructs plasmids

Measuring calibration factors by imaging a dish of cells expressing different tandem constructs plasmids

Reaction-based fluorescent and chemiluminescent probes for formaldehyde detection and imaging

A Ratiometric Fluorescent Nanoprobe for Ultrafast Detection of Formaldehyde in Wood and Food Samples

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