Bisulfite
(HSO3
–) is usually widely
added to tap water and food because it has antibacterial, bleaching,
and antioxidant effects. However, its abnormal addition would cause
a series of serious diseases related to it. Therefore, development
of an effective method for HSO3
– detection
was of great significance to human health. In this work, a new reaction-based
ratiometric fluorescent probe KQ–SO2 was rationally
designed, which could be used for the highly selective detection of
HSO3– in tap water, real food samples, onion tissues,
and zebrafish. Specifically, a positively charged benzo[e]indolium moiety and a carbazole group through a condensation reaction
resulted in KQ–SO2, which displayed two well-resolved
emission bands separated by 225 nm, fast response (1 min), and high
selectivity and sensitivity toward HSO3– upon undergoing
the Michael addition reaction, as well as low cytotoxicity in vitro.
In addition, KQ–SO2 has been successfully applied
for the detection of HSO3
– in tap water,
real food samples, onion tissues, and zebrafish with satisfactory
results. We predict that KQ–SO2 could be used as
a powerful tool to reveal the relationship between HSO3– and the human health.
Formaldehyde (FA, HCHO) is a highly reactive carbonyl species, which is very harmful to humans and the environment as a tissue fixative and preservative. Therefore, developing some highly sensitive, selective, and rapid detection methods is significant for human health in food safety and environmental protection. Herein, a two-photon (TP) ratiometric sensor, CmNp-CHO, has been constructed by conjugating a TP donor (Π-push−pull-structure) with a FA off−on acceptor (functioned with hydrazide moiety) via a nonconjugated linker through the fluorescence resonance energy transfer mechanism. Such a scaffold affords CmNp-CHO a reliable and specific probe for detecting FA with two well-resolved emission peaks separated by 124 nm. Also, it responds to FA rapidly with high selectivity and sensitivity during 1.0 min and a large ratio enhancement at I 550 /I 426 with addition of 0−20μM FA, exhibiting ∼4-fold ratio increase and a fairly low LOD of 8.3 ± 0.3 nM. Moreover, CmNp-CHO has been successfully employed for detecting FA in live cells, onion tissues, and zebrafish, exhibiting that CmNp-CHO can serve as a useful tool for investigating FA in real food application and offering strong theoretical support and technical means for investigation of physiological and pathological functions of FA.
Hydrogen sulfide (H2S) is one of the important gaseous signal molecules and plays key roles in various biologically crucial processes. In this work, we report a novel two-photon near-infrared (TP-NIR)...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.