A Novel Effective Formulation of Bioactive Compounds for Wound Healing: Preparation, In Vivo Characterization, and Comparison of Various Postbiotics Cold Creams in a Rat Model

Abstract

The wound is a break in the integrity of the skin produced by injury, illness, or operation. Wound healing is an essential dynamic biological/physiological process that occurs in response to tissue damage. The huge health, economic, and social effects of wounds on patients and societies necessitate the research to find novel potential therapeutic agents in order to promote wound healing. Postbiotics, the newest member of the biotics family, are valuable functional bioactive substances produced by probiotics through their metabolic activity, which have several beneficial properties, including immunomodulatory, anti-inflammatory, antimicrobial, and angiogenesis characteristics, resulting in acceleration of wound healing. In the current study, three topical cold cream formulations containing postbiotics obtained from Lactobacillus fermentum, Lactobacillus reuteri, or Bacillus subtilis sp. natto probiotic strains were prepared. The effectiveness and wound healing activity of the developed postbiotics cold cream formulations were investigated compared to cold cream without postbiotics and no treatment via wound closure investigation, hydroxyproline content assay, and histological assessment in 25 Sprague Dawley rats divided into five groups. Interestingly, analysis of the results revealed that all three formulations containing postbiotics significantly accelerated the wound healing process. However, in general, the Bacillus subtilis natto cold cream manifested a better wound healing property. The pleasing wound healing characteristics of the topical postbiotics cold creams through the in vivo experiment suggest that formulations containing postbiotics can be considered as a promising nominee for wound healing approaches.

Wound healing and microbiome, an unexpected relationship

“…natto showed improved wound healing process in an acute uninfected rat model. 49 Furthermore, prebiotics and postbiotics have been demonstrated to be effective in modulating inflammatory skin diseases that are associated with a dysbiosis, paving the way for the development of potential wound healing strategies. Notably, clinical applications have shown potential efficacy in the treatment of AD, which is a chronic, relapsing, intensely pruritic inflammatory skin disease, frequently associated with S. aureus overabundance.…”

Novel Wound Dressing Based on Postbiotic/Chitosan Film Accelerates Cutaneous Wound Healing

“…The therapeutic efficacy of Bacillus subtilis sp. natto, L. reuteri, and L. fermentum cold cream on rat wounds was investigated by Golkar et al (21). According to their findings, the formulation of Bacillus subtilis sp.…”

“…There has recently been a surge of interest in postbiotics, the newest members of the biotics family. Postbiotics are functional bioactive chemicals created during fermentation by the metabolic activity of probiotics that have direct or indirect positive effects on the host cells (21). Although postbiotics do not include living microorganisms, they positively impact host health through mechanisms similar to those seen in probiotics, reducing the hazards associated with their consumption.…”

The ameliorative role of specific probiotic combinations on bone loss in the ovariectomized rat model

“…They contain different microbes explained by their genus, species, and strain designation [ 7 ]. Probiotic supplements are beneficial for humans and animals and can be used after antibiotic therapy to compensate for gut microbiome deficiency or as prophylaxis [ 8 ]. The International Scientific Association of Probiotics and Prebiotics (ISAPP) has a helpful definition of probiotics consisting of three properties; probiotics should be alive during administration, be healthy for users, and have the right dose when delivered [ 9 ].…”