A Novel Disease of Big-Leaf Mahogany Caused by Two<i>Fusarium</i>Species in Mexico

Santillán-Mendoza, Ricardo; O’Donnell, Kerry; Ploetz, R. C.; Ortega-Arreola, Rubén; Vázquez-Marrufo, Gerardo; Benítez‐Malvido, Julieta; Montero-Castro, Juan Carlos; Soto-Plancarte, Alejandro; Rodríguez-Alvarado, Gerardo

doi:10.1094/pdis-01-18-0060-re

Cited by 19 publications

(9 citation statements)

References 45 publications

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“…The 94 fungal isolates were grown in dextrose potato broth (PDB) for 7 days, then the mycelium was compacted, dried, and ground with liquid nitrogen for DNA extraction using the CTAB protocol [11]. The DNA extracted was used to amplify the partial region of the TEF1 gene by PCR and the PCR products were resolved by electrophoresis in 1.5% agarose gel, later purified with Promega's Wizard SV Gel and PCR Clean-Up System kit and they ordered sequencing to the Potosí Institute for Scientific and Technological Research (San Luis Potosí, Mexico).…”

Section: Molecular Characterization Of Fungal Isolatesmentioning

confidence: 99%

Fungal species characterization associated with vanilla root rot in the Totonacapan, Veracruz, Mexico

Rosa¹,

Martínez-Gendrón²,

Torres-Olaya³

et al. 2023

Int. J. Sci. Res. Arch.

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Vanilla (Vanilla planifolia) is a crop of great biocultural importance in the Totonacapan region in Mexico; however, producers face various phytosanitary problems that decrease production. The main one being crop loss caused by the fungus, Fusarium oxysporum f. sp. vanillae, a pathogen that causes a disease whose symptomatology is root rot that in severe cases causes the death of plants. Therefore, the study of the pathogen is crucial, due to the scarcity of genetic variability of vanilla, a condition that makes the crops equally susceptible to the pathogen. The present work aimed to generate and molecularly and pathogenically characterize a fu-ngal isolates collection obtained from V. planifolia with root rot. Roots with rot were collected in six municipalities of the Totonacapan region, for fungal isolation, a semiselective culture medium was used, later, the molecular characterization was carried out through the amplification and sequencing of the partial region of the translational elongation factor 1α gene (TEF1). The pathogenic characterization was carried out on vanilla leaves in a humid chamber, inoculating medium discs with mycelium, and making observations for 18 days every 48 h. As results, 94 strains were obtained, of which 81 belong to the genus Fusarium (42 F. oxysporum strains), eight to Colletotrichum, three to Trichoderma and two it was not possible to identify them, only 23 Fusarium oxysporum strains showed to be pathogenic on vanilla leaves with different degrees of virulence. The results of the molecular and pathogenic characterization will be crucial for further studies of comparative genomics of Fusarium oxysporum.

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Section: Molecular Characterization Of Fungal Isolatesmentioning

confidence: 99%

Fungal species characterization associated with vanilla root rot in the Totonacapan, Veracruz, Mexico

Rosa¹,

Martínez-Gendrón²,

Torres-Olaya³

et al. 2023

Int. J. Sci. Res. Arch.

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“…However, studies reporting phytopathogenic action of the fungi E. endophytica (Douanla-Meli & Scharnhorst, 2021), N. cubana (Li, Li, & Yang, 2021), and F. pseudocircinatum (Santillán-Mendoza et al, 2018;Kee, et al, 2020) were found.…”

Section: Fungi Identificationmentioning

confidence: 99%

Fungal amylases applied to the sweet potato starch for bioethanol production

Romão

Filho

Tininis

et al. 2022

RSD

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Bioethanol is a sustainable energy source to help reducing the emission of pollutants into the global environment. In order to cope with that, the ethanol production technologies and use of efficient and low-cost substrates are developed. The objective of this research was to evaluate fungal amylases in sweet potato starch for bioethanol production by Saccharomyces cerevisiae in the laboratory. Endomelanconiopsis endophytica (1.40 U/mL), Neopestalotiopsis cubana (1.67 U/mL) and Fusarium pseudocircinatum (1.11 U/mL) with high enzymatic activities were selected and their amylases were tested for activity on sweet potato starch for bioethanol production. Simultaneous saccharification and fermentation was performed at 30° C and pH = 5.0. 17.3 - 88.1 (%) of bioethanol that was produced and compared to the expected theoretical yield. Therefore, amylases from these fungi simultaneously inserted on sweet potato starch and S. cerevisiae are potentially useful for bioethanol production.

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“…De estos se eligieron con base en la obtención de bandas claras y su reproducibilidad en todos los ensayos, a los oligonucleótidos denominados UBC807, UBC811, UBC814, UBC825, UBC841, UBC842 y UBC844 (Cuadro 2), anteriormente utilizados en varios estu- dios (Lau et al, 2005;Li y Jin, 2008;Muthusamy et al, 2008;Datta et al, 2010;Gama-Maia y Torres, 2016;George et al, 2017). Las condiciones de amplificación siguieron el procedimiento realizado por Santillán-Mendoza et al (2018). Los productos de los amplicones se corrieron en geles de agarosa al 1.5%, con TBE 0.5×, a cinco voltios por centímetro (5 V/cm) y un marcador de peso molecular de 100 pb (New England Biolabs, UK).…”

Section: Muestreo De Materials Vegetalunclassified

Variación genética de Balmea stormiae (Rubiaceae) en el centro-occidente de México

Mejía-Jiménez

Montero²,

González‐Rodríguez

et al. 2022

Acta Bot. Mex.

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Antecedentes y Objetivos: Los individuos de Balmea stormiae (Rubiaceae) son árboles o arbustos hemiepífitos que habitan malpaíses con bosques de pino-encino en poblaciones disyuntas en Centroamérica y México. La especie se considera en peligro de extinción y particularmente se ha documentado que las poblaciones de Michoacán han estado expuestas a fuertes presiones antropogénicas. Por tal motivo, en este estudio se estimó la diversidad genética en cuatro poblaciones de Michoacán, contrastando esta información con la obtenida en una población de Jalisco, ambas regiones ubicadas en el centro-occidente de México.Métodos: Las muestras vegetales fueron recolectadas en cinco poblaciones de la región occidente del Cinturón Volcánico Transmexicano. Para evaluar la diversidad genética, se utilizó el intrón rps16 del ADN de cloroplasto, el espaciador transcrito externo (ETS) del ADN ribosomal nuclear y marcadores dominantes ISSR de ADN nuclear (Inter Secuencias Simples Repetidas por sus siglas en inglés).Resultados clave: Los marcadores moleculares revelaron alta diferenciación genética entre las poblaciones de Jalisco y Michoacán, la cual se atribuye a la baja conectividad entre estas. Posiblemente se deba a la baja capacidad de dispersión a larga distancia de las semillas y el polen. Se detectó flujo genético moderado y expansión demográfica reciente en las poblaciones de Michoacán. Los niveles de variación genética en las poblaciones de Michoacán concuerdan con la edad de los flujos de lava, siendo Arocutín y Zacapu las poblaciones más diversas sobre los flujos de lava más antiguos.Conclusiones: Los resultados podrían explicarse por el origen relativamente reciente, producto de la actividad volcánica del Holoceno, de los ambientes en los que habita esta especie, sumado a la perturbación recurrente relacionada con grandes asentamientos prehispánicos de Michoacán.

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A Novel Disease of Big-Leaf Mahogany Caused by TwoFusariumSpecies in Mexico

Cited by 19 publications

References 45 publications

Fungal species characterization associated with vanilla root rot in the Totonacapan, Veracruz, Mexico

Fungal species characterization associated with vanilla root rot in the Totonacapan, Veracruz, Mexico

Fungal amylases applied to the sweet potato starch for bioethanol production

Variación genética de Balmea stormiae (Rubiaceae) en el centro-occidente de México

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