A Novel Culture Medium Using Lettuce Plant (Lactuca Sativa) For Detection of Pullulanase Production by Paenibacillus Macerans Isolated from Agricultural Wastes

Abstract

Pullulinase is an enzyme catalyzing the hydrolysis of pullulin forming maltotriose, panose and maltooligosaccharides that have a great prominence owing to their prospective use in medication, drink, diet and fine chemical industries. Because of the production of this enzyme is too expensive and characterized with low yield, there is a growing need to create a substrate to make the total process cheaper and more creative. In this study, the authors have reported pullulanase production from Paenibacillus macerans. Six isolates of Paenibacillus macerans were isolated from soil and agricultural wastes samples and screened for higher pullulanase production, Paenibacillus macerans isolated from second sample of wheat husk was gave superior inhibition zone around the colony as compared with other tested isolates. The activity of pullulanase was raised to 9.83U/ml when this strain was grown under the optimal conditions including using basal medium containing 1% (w/v) dried leaves of lettuce and 2% (w/v) peptone at pH 6, temperature of 50°C, inoculums size of 1.5 ml for 72 hour. The pullulanase exhibited highest activity (6.80U/ml) and (6.43U/ml) in presence of lettuce. Lettuce has aptitude as an efficient and economically suitable as compared with traditionally used substrates as pure pullulin and other types of saccharides.