Detection of Disinfectant property of purified Amylopullulanase from Citrobacter freundii SW

This research was carried out to study the bioactivitytest as disinfectant of two derivatives of organotin(IV) carboxylate compounds; diphenyltin(IV) di-2-chlorobenzoate (DPT2-CBz) (2) and triphenyltin(IV) 2-chlorobenzoate (TPT2-CBz) (4), against two pathogenic bacteria. Compounds 2 and 3 were successfully prepared by reactingdiphenyltin(IV) oxide (DPTO) (1) and triphenyltin(IV) hydroxide (TPTOH) (3) with 2-chlorobenzoic acid(2-HCBz)with yieldsof 87.71% and 88.73%, respectively. The characterization of the synthesized compounds was performed with several spectroscopy techniques such as UV, IR, 1HNMR, 13CNMR, and microelemental analyzer. The optical density (OD) test for both compounds measured using UV-Vis at 600 nm showeda goodbioactivity against two bacteria, Gram-negative Salmonella sp. and Gram-positive Staphylococcus aureus. The data revealed that compound 4 has bigger bioactivity than compound 2 and the positive control ofcommercial disinfectant (2.5% pine oil). The bioactivity of compound 4was optimum at concentration of 5x10-4 M and contact timeof 15 minutes.These data corresponding to the decrease of absorbance from 0.6640 to 0.0955 for Salmonella sp., and 0.6565 to 0.1295 for S. aureus, whereas although compound 2reached the optimum activityat the same concentration and contact time with compound 4, but the decrease of absorbance was less namely 0.6640 to 0.1305 for Salmonella sp., and 0.6565 to 0.196 for S. aureus. The decrease of absorbance in the measurement of OD implies that both compounds are potential to be used as disinfectant agent.

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Development of Technology for the production of finished forms of Medicinal probiotics

Gavrilova,

Ratnikova,

Sadanov

et al. 2023

RJPT

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The study aimed to develop a technology for the production of a finished form of medicinal probiotic agents against human intestinal infections based on active strains of lactic acid and propionic acid bacteria with a wide range of biological activity and resistance to antibiotics. From the laboratory collection of lactic acid and propionic acid bacteria isolated from the intestines of healthy people, two associations of bacteria were compiled with antagonism against test cultures of Staphylococcus aureus, Salmonella gallinarum, Mycobacterium B5, Candida albicans, Pasteurella multocida, Bacillus subtilis, Escherichia coli 8739, Klebsiella pneumoniae ATCC 700603 and ATCC BAA 2524, Staphylococcus aureus 3316 and 9, Salmonella enteritidis 35382, and Pseudomonas aeruginosa 835, as well as the ability to produce hydrolytic enzymes amylase and proteinase, B vitamins, and essential amino acids. The resistance of the selected associations of lactic acid and propionic acid bacteria to the used antibiotics has been studied, which will allow for using them, if necessary, in the complex therapy of diseases. Technology for the production of probiotic medication from these associations has been developed. It was found that the most active preparation in terms of bacterial titer and antagonistic activity and the most stable one during storage for 6 months was the liquid preparation obtained by growing association No. 2 (L. plantarum 2v/A-6+L. brevis B-3/A-26+L. acidophilus 27w/60+P. shermanii 8) on nutrient medium No. 1 (De Man, Rogosa and Sharpe agar with CoCl2) using 7% sucrose and 1.5% gelatin as a protector. The liquid preparation from association No. 5 grown on medium No. 1 showed a more complete preservation of production-valuable signs during storage compared to the results of using nutrient medium No. 4, while the use of protector No. 1 was more optimal. To test the stability during the storage of dry preparation forms, an accelerated method was used by warming them up for 15 minutes at 60°C. It was found that after warming up, the best preservation of viable bacterial cells was observed in association No. 2 on nutrient media No. 1 and No. 4, in association No. 5 on medium No. 4 dried with protector No. 2 (7% sucrose and 1.5% gelatin + 7% skim milk powder), while the titer of bacteria was equal to 1.2×109, 3.5×108, and 2.0±0.2×108 colony-forming units/g, respectively. Antagonistic activity in these association variants was observed against all test cultures taken into the study with zones of suppression of their growth ranging from 10 to 24 mm.

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Detection of Disinfectant property of purified Amylopullulanase from Citrobacter freundii SW

Cited by 3 publications

References 12 publications

Amylolytic lactic acid bacteria: Cell factories for direct lactic acid production from biomass by simultaneous saccharification and fermentation

Amylolytic lactic acid bacteria: Cell factories for direct lactic acid production from biomass by simultaneous saccharification and fermentation

Comparative Bioactivity Study of some Organotin (IV) 2-Chlorobenzoate compounds as Disinfectant agent

Development of Technology for the production of finished forms of Medicinal probiotics

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