A novel Bod-mediator biosensor based on Ferrocene and Debaryomyces hansenii yeast cells

Зайцева, Анна Сергеевна; Arlyapov, V. A.; Yudina, N.Yu.; Nosova, N. M.; Алферов, В. А.; Reshetilov, A. N.

doi:10.1134/s0003683817030152

Cited by 4 publications

(1 citation statement)

References 28 publications

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“…The use of mediator biosensors based on the use of compounds capable of reversible oxidation-reduction makes it possible to obtain results independent of the oxygen level in the analyzed sample and, in addition, to create devices quite small in size and weight ( Figure 8 , [ 40 , 41 , 42 ]). R&D resources in the field of mediator rapid BOD analyzers are directed in two directions: directly creating BOD biosensors based on the use of mediator electron transfer [ 43 , 44 , 45 , 46 ] and modifying the standard method of BOD analysis [ 47 , 48 , 49 ]. The possibility of modifying the standard method of BOD analysis using mediators was first presented by Pasco and co-authors in 2000 [ 50 ].…”

Section: Types Of Transducersmentioning

confidence: 99%

Microbial Biosensors for Rapid Determination of Biochemical Oxygen Demand: Approaches, Tendencies and Development Prospects

et al. 2022

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One of the main indices of the quality of water is the biochemical oxygen demand (BOD). A little over 40 years have passed since the practical application of the first microbial sensor for the determination of BOD, presented by the Japanese professor Isao Karube. This time span has brought new knowledge to and practical developments in the use of a wide range of microbial cells based on BOD biosensors. At present, this field of biotechnology is becoming an independent discipline. The traditional BOD analysis (BOD5) has not changed over many years; it takes no less than 5 days to carry out. Microbial biosensors can be used as an alternative technique for assessing the BOD attract attention because they can reduce hundredfold the time required to measure it. The review examines the experience of the creation and practical application of BOD biosensors accumulated by the international community. Special attention is paid to the use of multiple cell immobilization methods, signal registration techniques, mediators and cell consortia contained in the bioreceptor. We consider the use of nanomaterials in the modification of analytical devices developed for BOD evaluation and discuss the prospects of developing new practically important biosensor models.

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Section: Types Of Transducersmentioning

confidence: 99%

Microbial Biosensors for Rapid Determination of Biochemical Oxygen Demand: Approaches, Tendencies and Development Prospects

et al. 2022

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Electrochemical biochemical oxygen demand biosensors and their applications in aquatic environmental monitoring

Zhou,

Zheng,

Qin

2024

Sensing and Bio-Sensing Research

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A Two-Mediator System Based on a Nanocomposite of Redox-Active Polymer Poly(thionine) and SWCNT as an Effective Electron Carrier for Eukaryotic Microorganisms in Biosensor Analyzers

Medvedeva,

Dyakova,

Kuznetsova

et al. 2023

Polymers

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Electropolymerized thionine was used as a redox-active polymer to create a two-mediated microbial biosensor for determining biochemical oxygen demand (BOD). The electrochemical characteristics of the conducting system were studied by cyclic voltammetry and electrochemical impedance spectroscopy. It has been shown that the most promising in terms of the rate of interaction with the yeast B. adeninivorans is the system based on poly(thionine), single-walled carbon nanotubes (SWCNT), and neutral red (kint = 0.071 dm3/(g·s)). The biosensor based on this system is characterized by high sensitivity (the lower limit of determined BOD concentrations is 0.4 mgO2/dm3). Sample analysis by means of the developed analytical system showed that the results of the standard dilution method and those using the biosensor differed insignificantly. Thus, for the first time, the fundamental possibility of effectively using nanocomposite materials based on SWCNT and the redox-active polymer poly(thionine) as one of the components of two-mediator systems for electron transfer from yeast microorganisms to the electrode has been shown. It opens up prospects for creating stable and highly sensitive electrochemical systems based on eukaryotes.

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A novel Bod-mediator biosensor based on Ferrocene and Debaryomyces hansenii yeast cells

Cited by 4 publications

References 28 publications

Microbial Biosensors for Rapid Determination of Biochemical Oxygen Demand: Approaches, Tendencies and Development Prospects

Microbial Biosensors for Rapid Determination of Biochemical Oxygen Demand: Approaches, Tendencies and Development Prospects

Electrochemical biochemical oxygen demand biosensors and their applications in aquatic environmental monitoring

A Two-Mediator System Based on a Nanocomposite of Redox-Active Polymer Poly(thionine) and SWCNT as an Effective Electron Carrier for Eukaryotic Microorganisms in Biosensor Analyzers

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