Electrochemical biochemical oxygen demand biosensors and their applications in aquatic environmental monitoring

Zhou, Ying; Zheng, Shiling; Qin, Wei

doi:10.1016/j.sbsr.2024.100642

Cited by 2 publications

(2 citation statements)

References 126 publications

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“…This technology is widely used in microbial fuel cells (MFCs) [9,10]. The long-term stability of MFCs is one of the attractive advantages of using this technology in biosensor analysis, achieved through stable electroactive biofilms with high oxidative activity; it allows us to measure BOD for an average of 90-100 days [11]. The MFC substrate levels give two different types of output signaling concentrations: abstracted electrons (via the anode) and the production of protons (by the cathode), which allows MFCs to effectively determine the analyte concentration.…”

Section: Introductionmentioning

confidence: 99%

“…The MFC substrate levels give two different types of output signaling concentrations: abstracted electrons (via the anode) and the production of protons (by the cathode), which allows MFCs to effectively determine the analyte concentration. Response time is one of the challenges of this technology (the average response time is 140-180 min) [11], but the flow mode of MFCs is also an effective solution as a result of the optimization of the flow rate and the anode chamber volume [12,13]. Another way to achieve a rapid response on a small scale can be realized with bioanodes of a typical MFC with a counter Pd electrode and/or silver/silver chloride cell with an applied voltage; BOD is estimated through amperometric analysis (current-time plots) [14,15], but in this approach, planktonic systems are used, which are not as stable as biofilm MFCs.…”

Section: Introductionmentioning

confidence: 99%

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Targeted Formation of Biofilms on the Surface of Graphite Electrodes as an Effective Approach to the Development of Biosensors for Early Warning Systems

Kharkova,

Perchikov,

Kurbanalieva

et al. 2024

Biosensors

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Biofilms based on bacteria Pseudomonas veronii (Ps. veronii) and Escherichia coli (E. coli) and yeast Saccharomyces cerevisiae (S. cerevisiae) were used for novel biosensor creation for rapid biochemical oxygen demand (BOD) monitoring. Based on the electrochemical measurement results, it was shown that the endogenous mediator in the matrix of E. coli and Ps. veronii biofilms and ferrocene form a two-mediator system that improves electron transport in the system. Biofilms based on Ps. veronii and E. coli had a high biotechnological potential for BOD assessment; bioreceptors based on such biofilms had high sensitivity (the lower limits of detectable BOD5 concentrations were 0.61 (Ps. veronii) and 0.87 (E. coli) mg/dm3) and high efficiency of analysis (a measurement time 5–10 min). The maximum biosensor response based on bacterial biofilms has been observed in the pH range of 6.6–7.2. The greatest protective effect was found for biofilms based on E. coli, which has high long-term stability (151 days for Ps. veronii and 163 days for E. coli). The results of the BOD5 analysis of water samples obtained using the developed biosensors had a high correlation with the results of the standard 5-day method (R2 = 0.9820, number of tested samples is 10 for Ps. veronii, and R2 = 0.9862, number of tested samples is 10 for E. coli). Thus, biosensors based on Ps. veronii biofilms and E. coli biofilms could be a novel analytical system to give early warnings of pollution.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Targeted Formation of Biofilms on the Surface of Graphite Electrodes as an Effective Approach to the Development of Biosensors for Early Warning Systems

Kharkova,

Perchikov,

Kurbanalieva

et al. 2024

Biosensors

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A “2-in-1” Bioanalytical System Based on Nanocomposite Conductive Polymers for Early Detection of Surface Water Pollution

Kharkova,

Medvedeva,

Kuznetsova

et al. 2024

Polymers

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This work proposes an approach to the formation of receptor elements for the rapid diagnosis of the state of surface waters according to two indicators: the biochemical oxygen demand (BOD) index and toxicity. Associations among microorganisms based on the bacteria P. yeei and yeast S. cerevisiae, as well as associations of the yeasts O. polymorpha and B. adeninivorans, were formed to evaluate these indicators, respectively. The use of nanocomposite electrically conductive materials based on carbon nanotubes, biocompatible natural polymers—chitosan and bovine serum albumin cross-linked with ferrocenecarboxaldehyde, neutral red, safranin, and phenosafranin—has made it possible to expand the analytical capabilities of receptor systems. Redox polymers were studied by IR spectroscopy and Raman spectroscopy, the contents of electroactive components were determined by atomic absorption spectroscopy, and electrochemical properties were studied by electrochemical impedance and cyclic voltammetry methods. Based on the proposed kinetic approach to modeling individual stages of bioelectrochemical processes, the chitosan–neutral red/CNT composite was chosen to immobilize the yeast association between O. polymorpha (ks = 370 ± 20 L/g × s) and B. adeninivorans (320 ± 30 L/g × s), and a bovine serum albumin (BSA)–neutral composite was chosen to immobilize the association between the yeast S. cerevisiae (ks = 130 ± 10 L/g × s) and the bacteria P. yeei red/CNT (170 ± 30 L/g × s). After optimizing the composition of the receptor systems, it was shown that the use of nanocomposite materials together with associations among microorganisms makes it possible to determine BOD with high sensitivity (with a lower limit of 0.6 mg/dm3) and detect the presence of a wide range of toxicants of both organic and inorganic origin. Both receptor elements were tested on water samples, showing a high correlation between the results of biosensor analysis of BOD and toxicity and the results of standard analytical methods. The results obtained show broad prospects for creating sensitive and portable bioelectrochemical sensors for the early warning of environmentally hazardous situations based on associations among microorganisms and nanocomposite materials.

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Electrochemical biochemical oxygen demand biosensors and their applications in aquatic environmental monitoring

Cited by 2 publications

References 126 publications

Targeted Formation of Biofilms on the Surface of Graphite Electrodes as an Effective Approach to the Development of Biosensors for Early Warning Systems

Targeted Formation of Biofilms on the Surface of Graphite Electrodes as an Effective Approach to the Development of Biosensors for Early Warning Systems

A “2-in-1” Bioanalytical System Based on Nanocomposite Conductive Polymers for Early Detection of Surface Water Pollution

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