A novel Bacillus sp. isolated from rotten seaweed: Identification and characterization alginate lyase its produced

Abstract

Abstract. Zilda DS, Yulianti Y, Sholihah RF, Subaryono S, Fawzya YN, Irianto HE.2019. A novel Bacillus sp. isolated from rotten seaweed: identification and characterization alginate lyase it is produced. Biodiversitas 20: 1166-1172. Alginate lyase has been known as potential biocatalyst not only for industrial but also medicinal application especially for the production of oligosaccharides which have distinct bioactivities. An alginate lyase, AlgT513, has been isolated from rotten seaweed bacterium strain T513 and characterized. The bacterium showed low similarity (95%) with Bacillus tequilensis strain 10b based on 16S rDNA sequence indicating that Alg07 may be a novel Bacillus species. The bacterium forms a clear zone on solid medium with 0.5% sodium alginate addition. The optimum temperature and pH were 50ºC and 8 respectively. AlgT513 maintained stability at board pHs of 4-9 and temperature of 45ºC. Metal ions Mg2+, Ca2+ and K+ increase the activity of the enzyme while Zn2+, Co2+ and Li+ strongly inhibit it. NaCl inhibits AlgT513 activity where most of the alginate lyases need it to reach maximum activity. AlgT513 is suggested as a serine metalloenzyme due to inhibition of ethylenediaminetetraacetic acid (EDTA) and phenylmethylsulfonyl fluoride (PMSF).

Biotechnologically potential genes in a polysaccharide-degrading epibiont of the Indonesian brown algae Hydroclathrus sp.

“…The colonies growing on solid MS were isolated and purified. The ability of the bacterial isolates to degrade polysaccharides was assessed by a 24-h incubation and subsequent staining with Lugol’s iodine solution, followed by staining with CaCl 2 solution (10%) with 1-h incubation to observe the capability of degrading alginate [ 70 ]. The most potential bacterial isolate was subsequently tested for the antimicrobial ability of its extracellular fraction against Staphylococcus aureus ATCC®25923™, an opportunistic bacterial pathogen that can cause various infections and food poisoning [ 14 , 63 ].…”

“…In search for biotechnologically potential genes from seaweed epibionts, we initially isolated a novel polysaccharide-degrading bacterial species associated with the Indonesian brown algae Hydroclathrus sp. [ 70 ]. We found that the cell-free culture of this epibiont was able to inhibit the bacterial pathogen S. aureus , indicating its potential ability to produce antimicrobial substance extracellularly.…”

Alginate Lyases from Marine Bacteria: An Enzyme Ocean for Sustainable Future

“…[ 84 ], Defluviitalea phaphyphila [ 85 ], Klebsiella aerogenes type 25 [ 86 ], Pseudomonas alginovora XO17 [ 87 ], Bacillus sp. [ 42 , 88 ], Corynebacterium sp. ALY-1 [ 89 ], Zobellia galactanivorans [ 90 ], and Agarivorans sp.…”

“…A couple of alginate lyases were separated from different kinds of microorganisms in the past several years, especially from the bacteria on brown algae (such as Bacillus sp. obtained from rotten seaweed) [ 88 ], Paenibacillus algicola isolated from rotten brown algae samples collected from China [ 92 ], and Pseudoalteromonas sp. SM0524 separated from marine kelp residues [ 93 ].…”

Short Communication: Diversity of Chlorpyrifos-degrading bacteria isolated from shallow aquifer of East Java Coastal Settlements, Indonesia

“…Bacillus genera are recognized for the making of wide range of medicinal, agricultural, pharmaceutical and industrial products, example.g., Bacillus tequilensis as alginate lyase producer (Zilda et al 2019), Bacillus subtilis MA9 produce thermostable α-amylase enzyme (Devi et al 2010), Bacillus cereus KKT 1, B. cereus KKT 14, B. cereus KKT 19. Bacillus thuringiensis KKT 6, produce chitin-degrading enzyme (Puspita et al 2017) and B. cereus, B. subtilis B. thuringiensis, and B. pumilus as antibiotic-producing agent (Amin et al 2015;Magda and Hamed 2016).…”