A novel avian retrovirus associated with lymphocytoma isolated from a local Chinese flock induced significantly reduced growth and immune suppression in SPF chickens

Wu, Xiaoping; Zhao, Jing; Zeng, Yukun; Wu, Yijian; Wang, Quanxi; Wu, Benhong; Huang, Yifan

doi:10.1016/j.vetmic.2017.04.011

Cited by 13 publications

(7 citation statements)

References 20 publications

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“…Until now, there have not been any reports on the effect of BLPs on animal humoral immune responses against active vaccines. In a previous report, we found that the vertical infection of the recombinant ALV strain FJ15HT0 in chickens exerted a negative effect on the humoral immune response to active vaccines but not to inactive vaccines [3]. Coincidentally, in this report we found that injection of our novel BLP maintained the antibody titer peak against the active NDV vaccine in ALV FJ15HT0 infected chickens but not mock birds (Fig.…”

Section: Discussionsupporting

confidence: 63%

“…The recently isolated recombinant ALV FJ15HT0 [3], which associated with immunity and growth suppression in infected chickens, was used in this study.…”

Section: Methodsmentioning

confidence: 99%

“…Avian leukosis viruses (ALVs) are divided into 6 subgroups (A, B, C, D, E and J) in chicken flocks. It can cause diverse tumors [1], immunity suppression [2] and growth performance inefficiency [3], resulting in great economic losses. Until now, various eradication and control programs have been successfully developed and applied, including antibody testing, antigen testing, virus isolation [1], hatching and rearing chickens in small groups [4], and different vaccine preparations [5].…”

Section: Introductionmentioning

confidence: 99%

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A novel Bursin-like peptide as a potential virus inhibitor and immunity regulator in SPF chickens infected with recombinant ALV

Zeng

Gong

et al. 2019

BMC Vet Res

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BackgroundAvian leukosis viruses (ALVs) are important contagious suppressive factors of chicken immunity and growth performance, resulted in enormous economic loss. Although virus eradication programs are applied in breeder flocks, ALVs are still widespread globally. Therefore, other valuable adjunct to reduce the negative effect of ALVs should be considered. Bursin-like peptide (BLP) showed remarkable immunomodulatory effects, whereas their influence on ALV-infected avian groups has not been reported. Here, a designed hybrid BLP was expressed in E. coli. The purified BLP was injected subcutaneously weekly in SPF chickens congenitally infected with a natural ALV strain. Then the influences of this BLP on the growth performance, immune response and virus titer of ALV-infected chickens were determined.ResultsThis BLP injection significantly improved the body weights of ALV-infected birds (P < 0.05). BLP injection significantly enhanced organ index in the BF in ALV-infected birds (P < 0.05). The weekly injection of BLP significantly lengthened the maintenance time of antibodies against Newcastle disease virus (NDV) attenuated vaccine of ALV-infected birds (P < 0.05) and boosted the antibody titer against avian influenza virus (AIV) H5 inactive vaccine of mock chicken (P < 0.05). BLP injection in mock chickens enhanced the levels of serum cytokines (IL-2, IL-4 and interferon-γ) (P < 0.05). Surprisingly, the novel BLP significantly inhibited expression of the ALV gp85 gene in the thymus (P < 0.05), kidney (P < 0.05) and bursa of Fabricius (BF) (P < 0.01) of ALV-infected chickens. Both viral RNA copy number and protein level decreased significantly with BLP (50 μg/mL) inoculation before ALV infection in DF1 cells (P < 0.05).ConclusionsThis is the first report investigating the influence of BLP on the growth and immunity performance of chickens infected by ALV. It also is the first report about the antiviral effect of BLP in vivo and in vitro. This BLP expressed in E. coli showed potential as a vaccine adjuvant, growth regulator and antiretroviral drug in chickens to decrease the negative effects of ALV infection.

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Section: Discussionsupporting

confidence: 63%

“…The recently isolated recombinant ALV FJ15HT0 [3], which associated with immunity and growth suppression in infected chickens, was used in this study.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A novel Bursin-like peptide as a potential virus inhibitor and immunity regulator in SPF chickens infected with recombinant ALV

Zeng

Gong

et al. 2019

BMC Vet Res

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“…In addition, with the spread of ALV-J in local breeds of chickens in China, ALV-J and other subpopulations of recombinant viruses have emerged one after another. For example FJ15HT0 (Wu et al, 2017), consisting of an ALV-B gp85 gene, an ALV-E gp37 and a J subgroup LTR region induced significantly reduced growth and immune suppression in SPF chickens. Also, HLJ13SH01 (Gao et al, 2015) and GX14FF03 (Wang et al, 2017) and LC110515–5, were identified.…”

Section: Discussionmentioning

confidence: 99%

A novel recombinant avian leukosis virus isolated from gamecocks induced pathogenicity in Three-Yellow chickens: a potential infection source of avian leukosis virus to the commercial chickens

Wang

Shi

He³

et al. 2019

Poultry Science

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One natural recombinant avian leukosis virus (ALV) strain GX14DJ3–18 was isolated from a native gamecock by DF-1 cell culture and identified with Polymerase Chain Reaction (PCR), immunofluorescence assay and the viral genome's nucleotide sequencing. This strain was revealed as a novel recombinant virus with nucleotide sequence similarities of 95.4% Long Terminal Repeated (LTR), 95.8% 5′, UTR, 97.9% gag, and 92.9% 3′untranslated regions (UTR) in ALV-J. Also we found sequence similarities of 99.3% pol and 99.0% gp37 in ALV-E, and 89.9% gp85 in ALV-A. The simulated congenital infection with GX14DJ3–18 in Three-Yellow chickens exhibited a significant negative effect on the development of immune organs (P < 0.05). Also, lower antibody responses were found to vaccinations with the commercial vaccines of Newcastle disease virus and with subtypes H5 and H9 of avian influenza virus (P < 0.05). The incidence of tumor or tumor-like lesions in the challenged birds was 14.28% (5/35), while none were observed in the un-challenged control group (0/35). These results suggested that GX14DJ3–18 is a novel recombinant ALV that can induce pathogenicity in the commercial Three-Yellow chickens. We speculated that cross-provincial sales of gamecocks in which ALVs have not been eradicated thoroughly might be a potential route for the transmission of ALVs to commercial chickens.

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“…Twenty‐four hours later, the diluted vaccine samples were inoculated into DF‐1 cells for 2 hr, then replenished with DMEM containing 1% foetal calf serum (Life, Australia) and passed for three serial passages (7 days for each passage). Then, the cell cultures were used as the sample for the identifications of ALV by ELISA, PCR and IFA, respectively (Wu et al., ).…”

Section: Methodsmentioning

confidence: 99%

Full-length genome sequence analysis of an avian leukosis virus subgroup J (ALV-J) as contaminant in live poultry vaccine: The commercial live vaccines might be a potential route for ALV-J transmission

Wang

Lin

et al. 2018

Transbound Emerg Dis

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One avian leukosis virus subgroup J (ALV-J) strain was isolated from 67 commercial live poultry vaccines produced by various manufacturers during 2013-2016 in China. The complete genomes of the isolate were sequenced and it was found that the genes gag and pol of the strain were relatively conservative, while the gp85 gene of the strain GX14YYA1 had the highest similarities with a field strain GX14ZS14, which was isolated from the chickens of a farm that had once used the same vaccine as the one found to be contaminated with the GX14YYA1. This is the first report of ALV-J contaminant in live poultry vaccine in China. Our finding demonstrates that vaccination of the commercial live vaccines might be a potential new route for ALV-J transmission in chickens and highlights the need for more extensive monitoring of the commercial live vaccines in China.

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A novel avian retrovirus associated with lymphocytoma isolated from a local Chinese flock induced significantly reduced growth and immune suppression in SPF chickens

Cited by 13 publications

References 20 publications

A novel Bursin-like peptide as a potential virus inhibitor and immunity regulator in SPF chickens infected with recombinant ALV

A novel Bursin-like peptide as a potential virus inhibitor and immunity regulator in SPF chickens infected with recombinant ALV

A novel recombinant avian leukosis virus isolated from gamecocks induced pathogenicity in Three-Yellow chickens: a potential infection source of avian leukosis virus to the commercial chickens

Full-length genome sequence analysis of an avian leukosis virus subgroup J (ALV-J) as contaminant in live poultry vaccine: The commercial live vaccines might be a potential route for ALV-J transmission

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