Full-length genome sequence analysis of an avian leukosis virus subgroup J (ALV-J) as contaminant in live poultry vaccine: The commercial live vaccines might be a potential route for ALV-J transmission

Wang, P.; Lin, Li-Chun; Li, H.; Shi, Mi; Gu, Zhanming; Wei, Ping

doi:10.1111/tbed.12841

Cited by 25 publications

(14 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mutations of the new isolated ALV‐J strains, occurring in conserved regions, may be one of the factors for more severe oncogenicity. Besides mutation of virus, it also has other contributing reasons for the outbreak of avian leukosis, such as the vaccination of ALV‐contaminated live vaccines (Wang et al., ). Thus, the biocharacteristics, pathogenesis and novel control measures of ALV‐J should be further studied.…”

Section: Resultsmentioning

confidence: 99%

“…Values along the branches indicate bootstrap values. The marker denotes a measurement of the relative phylogenetic distance [Colour figure can be viewed at wileyonlinelibrary.com] reasons for the outbreak of avian leukosis, such as the vaccination of ALV-contaminated live vaccines (Wang et al, 2018). Thus, the biocharacteristics, pathogenesis and novel control measures of ALV-J should be further studied.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Outbreak of myelocytomatosis caused by mutational avian leukosis virus subgroup J in China, 2018

Zhou

Xue

Zhang

et al. 2018

Transbound Emerg Dis

View full text Add to dashboard Cite

Avian leukosis virus subgroup J (ALV‐J) was isolated in meat‐type breeder chickens for the first time in 1988 in the United Kingdom. Due to the application of an eradication program, there were fewer reports related to myelocytomatosis or ALV‐J in China after 2013. However, there was another breakout almost simultaneously in six provinces of China in February 2018. On‐site, 15‐ to 20‐week‐old broiler breeder chickens showed depression, paralysis and weight loss. Mortality for certain flocks reached 15%. Sick chickens showed numerous yellow–white neoplasms growing in the sternum, rib and lumbar vertebra and had hepatic and renal metastasis. Histopathological observation showed all neoplasms were myelocytomas, and there were massive myelocyte‐like tumour cells in the liver, kidney and bone marrow. To explore the aetiology of this re‐outbreak of myelocytomatosis in China, we collected tumour‐bearing chickens and isolated six strains of ALV‐J (GM0209‐1 to ‐6). Phylogenetic analysis of gp85 and gp37 showed GM0209 strains were clearly distinct from the prototype strain of ADOL‐7501, HPRS‐103 and NX0101, and there was a mutation, R176G, in the conserved region between hr1 and hr2 regions of gp85, which was not found in other 44 ALV‐J strains. The 3′UTR nucleotide sequences of GM0209 isolates showed there was a signature deletion of 11 nt that was also present in 3′UTR sequences of SCDY1 and NHH, two isolates that have a reported association with haemangioma, indicating this deletion could not determine the tumour type induced by ALV‐J. Although the eradication program of ALV‐J has been successfully applied in China, the outbreak of ALV‐J still occurred, and the virus strain spread quickly. Thus, the biocharacteristics and pathogenesis of mutational ALV‐J should be further studied.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Outbreak of myelocytomatosis caused by mutational avian leukosis virus subgroup J in China, 2018

Zhou

Xue

Zhang

et al. 2018

Transbound Emerg Dis

View full text Add to dashboard Cite

show abstract

“…ALVs can be transmitted vertically or horizontally (Lin et al, 2013) and the commercial live vaccine might also be a potential route for ALVs transmission (Wang et al, 2018a). In this study, a strain that isolated from gamecocks was found to cause severe immunosuppression and tumor incidence in the commercial Three-Yellow chicken.…”

Section: Discussionmentioning

confidence: 99%

“…Avian leukosis viruses ( ALVs ) are known as a variety of infectious retroviral agents that cause severe economic loss to the poultry industry worldwide (Payne and Nair, 2012). Avian leukosis viruses that infect chickens comprise 7 envelope subgroups designated A to E, J and K (Wang et al, 2018a). Subgroups A, B, C, D, J, and K are exogenous ALVs while subgroup E is endogenous (Su et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

A novel recombinant avian leukosis virus isolated from gamecocks induced pathogenicity in Three-Yellow chickens: a potential infection source of avian leukosis virus to the commercial chickens

Wang

Shi

He³

et al. 2019

Poultry Science

View full text Add to dashboard Cite

One natural recombinant avian leukosis virus (ALV) strain GX14DJ3–18 was isolated from a native gamecock by DF-1 cell culture and identified with Polymerase Chain Reaction (PCR), immunofluorescence assay and the viral genome's nucleotide sequencing. This strain was revealed as a novel recombinant virus with nucleotide sequence similarities of 95.4% Long Terminal Repeated (LTR), 95.8% 5′, UTR, 97.9% gag, and 92.9% 3′untranslated regions (UTR) in ALV-J. Also we found sequence similarities of 99.3% pol and 99.0% gp37 in ALV-E, and 89.9% gp85 in ALV-A. The simulated congenital infection with GX14DJ3–18 in Three-Yellow chickens exhibited a significant negative effect on the development of immune organs (P < 0.05). Also, lower antibody responses were found to vaccinations with the commercial vaccines of Newcastle disease virus and with subtypes H5 and H9 of avian influenza virus (P < 0.05). The incidence of tumor or tumor-like lesions in the challenged birds was 14.28% (5/35), while none were observed in the un-challenged control group (0/35). These results suggested that GX14DJ3–18 is a novel recombinant ALV that can induce pathogenicity in the commercial Three-Yellow chickens. We speculated that cross-provincial sales of gamecocks in which ALVs have not been eradicated thoroughly might be a potential route for the transmission of ALVs to commercial chickens.

show abstract

“…Avian leukosis virus belongs to the genus Alpharetroviru s and the family Retroviridae ( Wang et al., 2019 ). The proviral DNA arrangement of the genome of ALV was 5′LTR-leader- gag - pol - env -3′LTR and the full-length of the genome sequences of ALV was 7,200 bp-7,800 bp ( Wang et al., 2018a ). The LTR is the nonstructural gene, whereas gag , pol, and env are the structural genes ( Meng et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Full-length cDNA sequence analysis of 85 avian leukosis virus subgroup J strains isolated from chickens in China during the years 1988–2018: coexistence of 2 extremely different clusters that are highly dependent upon either the host genetic background or the geographic location

Wang

et al. 2020

Poultry Science

View full text Add to dashboard Cite

During the process of transmission and spread of avian leukosis virus subgroup J ( ALV-J ) in chickens worldwide, the viral genome is constantly changing. A comprehensive and systematic study of the evolutionary process of ALV-J in China is needed. In this study, we amplified the full-length viral cDNA sequences of 16 ALV-J isolates of Yellow-chicken origin and analyzed and compared these sequences with another 69 ALV-J strains isolated during the years 1988–2018. These isolates were then sorted into 2 clusters: cluster I included isolates that mainly originated from the layers and White-feather broilers from northern China; cluster II included isolates mainly from the Yellow-chicken, most of them being from southern China. According to the sequence homologies of the whole genome and gag , pol , gp85, and gp37 genes, the ALV-J strains are more likely to randomly change in different directions from the original strain HPRS-103 as time passes. The results of entropy analysis of the sequences of gag , pol, and env revealed that the env gene had the largest variation, and the gag gene nonconserved sites are mainly concentrated in p19, p10, and p12. In addition, 84.71% (72/85) of the isolates had the 205-nucleotide (nt) deletion in the 3′UTR region, and 30.59% (26/85) of the isolates had the 125-nt to 127-nt deletion in the E element. Our study provides evidence for the coexistence of 2 extremely different clusters of ALV-J prevailing in China and in some other countries during the period of 1988–2018 and implies that the clusters are highly dependent on the host genetic background and the geographic location.

show abstract

Full-length genome sequence analysis of an avian leukosis virus subgroup J (ALV-J) as contaminant in live poultry vaccine: The commercial live vaccines might be a potential route for ALV-J transmission

Cited by 25 publications

References 18 publications

Outbreak of myelocytomatosis caused by mutational avian leukosis virus subgroup J in China, 2018

Outbreak of myelocytomatosis caused by mutational avian leukosis virus subgroup J in China, 2018

A novel recombinant avian leukosis virus isolated from gamecocks induced pathogenicity in Three-Yellow chickens: a potential infection source of avian leukosis virus to the commercial chickens

Full-length cDNA sequence analysis of 85 avian leukosis virus subgroup J strains isolated from chickens in China during the years 1988–2018: coexistence of 2 extremely different clusters that are highly dependent upon either the host genetic background or the geographic location

Contact Info

Product

Resources

About