A Novel Approach via Surface Modification of Degradable Polymers With Adhesive DOPA-IGF-1 for Neural Tissue Engineering

Abstract

The highly damaging state of spinal cord injuries has provided much inspiration for the design of surface modification of the implants that can promote nerve regeneration and functional reconstruction. DOPA-IGF-1, a new recombinant protein designed in our previous study, exhibited strong binding affinity to titanium and significantly enhanced the growth of NIH3T3 cells on the surface of titanium with the same biological activity as IGF-1. In this article, surface modification of poly(lactide-co-glycolide) (PLGA) films with recombinant DOPA-IGF-1 was performed to promote the paracrine activity of human umbilical cord mesenchymal stem cells (hUCMSCs) by secreting neurotrophic factors. DOPA-IGF-1 exhibited the strongest binding ability to PLGA films than commercial IGF-1 and nonhydroxylated YKYKY-IGF-1. In vitro cultures of hUCMSCs on the modified PLGA films showed that DOPA-IGF-1@PLGA substrates significantly improved the proliferation, adhesion, and neurotrophic factors secretion of hUCMSCs, especially for nerve growth factor, as confirmed by qRT-PCR and western blot analysis. Subsequently, the acquired neurotrophic factors secreted by the hUCMSCs cultured on the DOPA-IGF-1@PLGA films obviously enhanced neurite outgrowth of PC12 cells. Taken together, PLGA substrates with DOPA-IGF-1 immobilization is a promising platform for neural tissue engineering via neurotrophic factors secretion from MSCs and should be further tested in vivo.

Recent Advances in Bioplastics: Application and Biodegradation

“…Bianco et al 2011 [157] and D'Angelo et al 2012 [158] showed that electrospun PLLA/dHA could differentiate toward the osteogenic lineage human (bone marrow derived) hMSCs or even induced pluripotent adult or embryonic stem cells (iPSC and ESC) of murine model. hMSCs were also used by Zhang and colleagues [159] to induce the production of neurotrophic factors after the successful plating on DOPA-IGF-1 bound PLGA scaffold. The successful production of neurotrophic growth factor allowed PC12 cells to have a sustained neurite outgrowth.…”

Dual bio-active factors with adhesion function modified electrospun fibrous scaffold for skin wound and infections therapeutics

“…This phenomenon demonstrates that DA, which contains hydrophilic groups, was successfully introduced into the bioactive factor. Many studies have found that DA can make the surface of polymer materials more hydrophilic, due to the presence of various hydrophilic groups in its structure, such as catechol, quinone and amine 32,33 . We also tested the tensile strengths of the different electrospun fibrous scaffolds.…”

Acceleration of Healing in Full-Thickness Wound by Chitosan-Binding bFGF and Antimicrobial Peptide Modification Chitosan Membrane

“…For example, some studies prepared recombinant bFGF with collagen-anchoring ability to enhance the binding of bFGF and collagen scaffold, and the result indicated that this recombinant bFGF can obviously promote cell proliferation compared with commercial bFGF ( Wu et al, 2018 ). ( Zhang et al, 2019 ) introduced the DOPA domain into growth factor (IGF-1) via recombinant DNA technology and found that recombinant IGF-1 can be tightly bound to the surface of PLGA film ( Zhang et al, 2019 ). More importantly, this recombinant IGF-1 can promote mesenchymal stem cells to secrete neurotrophic factors and provide a suitable cellular microenvironment for nerve cell growth.…”