Acceleration of Healing in Full-Thickness Wound by Chitosan-Binding bFGF and Antimicrobial Peptide Modification Chitosan Membrane

Abstract

Skin wound healing is an important clinical challenge, and the main treatment points are accelerating epidermal regeneration and preventing infection. Therefore, it is necessary to develop a wound dressing that can simultaneously cure bacterial infections and accelerate wound healing. Here, we report a multifunctional composite wound dressing loaded with chitosan (CS)-binding bFGF (CSBD-bFGF) and antimicrobial peptides (P5S9K). First, CS was used as the dressing matrix material, and P5S9K was encapsulated in CS. Then, CSBD-bFGF was designed by combining recombinant DNA technology and tyrosinase treatment and modified on the dressing material surface. The results show that the binding ability of CSBD-bFGF and CS was significantly improved compared with that of commercial bFGF, and CSBD-bFGF could be controllably released from the CS dressing. More importantly, the prepared dressing material showed excellent antibacterial activity in vivo and in vitro and could effectively inhibit the growth of E. coli and S. aureus. Using NIH3T3 cells as cellular models, the results showed that the CSBD-bFGF@CS/P5S9K composite dressing was a friendly material for cell growth. After cells were seeded on the composite dressing surface, collagen-1 (COL-1) and vascular endothelial growth factor (VEGF) genes expression in cells were significantly upregulated. Finally, the full-thickness wound of the rat dorsal model was applied to analyse the tissue repair ability of the composite dressing. The results showed that the composite dressing containing CSBD-bFGF and P5S9K had the strongest ability to repair skin wounds. Therefore, the CSBD-bFGF@CS/P5S9K composite dressing has good antibacterial and accelerated wound healing abilities and has good application prospects in the treatment of skin wounds.

Nanoscale borosilicate bioactive glass for regenerative therapy of full-thickness skin defects in rabbit animal model

“…Analysis of epithelial thickness was performed using ImageJ software (NIH, United States), where a skilled histologist blindly evaluated the analyses of tissue slices by choosing three random, non-overlapping areas at a magnification of ×10 per tissue section. The epithelial gap as well as percentage of reduction in wound size were measured using four panoramic images of different histological sections following the protocol described by Hou et al (2022) .…”

Accelerated Healing of Infected Diabetic Wounds by a Dual-Layered Adhesive Film Cored with Microsphere-Loaded Hydrogel Composite Dressing

“…After 5 days of treatment, the expression of collagen was significantly increased . In another study, Hou et al also reported that bFGF (500 ng/mL)-bound chitosan membranes could upregulate the expression of collagen after 4 days of treatment . In the present study, we also combined similar concentrations of bFGF (500 ng/mL) with SA microspheres to construct the hydrogel composite to promote collagen expression (Figure ).…”

“…66 In another study, Hou et al also reported that bFGF (500 ng/mL)-bound chitosan membranes could upregulate the expression of collagen after 4 days of treatment. 18 In the present study, we also combined similar concentrations of bFGF (500 ng/mL) with SA microspheres to construct the hydrogel composite to promote collagen expression (Figure 6). Jia et al noted a reverse correlation between the concentration of bFGF (10−100 ng/ mL) and the effect on the proliferation of rat adipose mesenchymal stem cells and the expression of collagen after 7 days of stimulation.…”

“…As a single-chain polypeptide composed of 146 amino acids, a basic fibroblast growth factor (bFGF) is essential for tissue regeneration, which may contribute to cell migration, proliferation, and angiogenesis, and has already been applied to the treatment of chronic wounds. , During the process of wound healing, bFGF can stimulate fibroblasts to secrete type I and type III collagens . However, its short half-life, enzymatic degradation, and ineffective diffusion have limited its applications .…”

"Simmer pus and grow flesh" method promotes chronic wound healing in rats via bFGF-Wnt β-catenin signaling pathway

“…In addition, Zibai ointment can improve the microenvironment of the wound, keep the wound moist and provide an environment conducive to cell growth and wound healing. And bFGF, as an important growth factor, plays a momentous effect in wound healing [13][14][15]. The wnt/β-catenin signaling pathway has multiple functions in wound healing, including promoting epithelial cell proliferation and migration, promoting angiogenesis, and regulating inflammatory response [16,17].…”