A novel antioxidant agent, astragalosides, prevents shock wave-induced renal oxidative injury in rabbits

Li, Xiang; He, Dalin; Zhang, Linlin; Cheng, Xinfa; Sheng, Binwu; Luo, Yunping

doi:10.1007/s00240-006-0057-1

Cited by 41 publications

(27 citation statements)

References 30 publications

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“…[4][5][6] Astragaloside IV exhibits various pharmacological properties, such as anti-inflammatory, [7] anti-viral, [8] anti-aging, [9] anti-oxidant, [10] and so on. Cycloastragenol could delay the onset of cellular aging by increasing telomerase activity, [11] up-regulate the immune system by inducing IL-2 release, [12] and enhance the antiviral function of human CD8…”

Section: Introductionmentioning

confidence: 99%

Biocatalysis of Cycloastragenol by Filamentous Fungi to Produce Unexpected Triterpenes

Yang

Huang

et al. 2012

Adv Synth Catal

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The biocatalysis of cycloastragenol, a natural tetracyclic triterpenoid with anti-aging activity, by cultured whole cells of three strains of filamentous fungi, namely Cunninghamella elegans AS 3.1207, Syncephalastrum racemosum AS 3.264 and Doratomyces stemonitis AS 3.1411 produced 15 metabolites. Thirteen of them are new compounds. The structures of these metabolites were fully characterized on the basis of HR-ESI-MS analyses together with 1D and 2D NMR spectroscopy. The three fungal strains exhibited significant biocatalytic preferences: C. elegans enabled hydroxylation reactions, particularly on the 28-and 29-CH 3 groups; S. racemosum efficiently catalyzed a complicated rearrangement reaction to form the unusual ranunculane skeleton, which was further substituted with diverse side chains at C-19; D. stemonitis mainly led to carbonylation reactions, especially on 3-OH. It is particularly noteworthy that S. racemosum also catalyzed an unexpected ring expansion reaction to generate the rare 9(10)a-homo-19-nor-cycloartane skeleton. Biocatalysis was proved powerful in the structural diversification of cycloastragenol for future structure-activity relationship studies.

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Section: Introductionmentioning

confidence: 99%

Biocatalysis of Cycloastragenol by Filamentous Fungi to Produce Unexpected Triterpenes

Yang

Huang

et al. 2012

Adv Synth Catal

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“…Moreover, it has been reported that herbal formulations containing Radix Astragali and some of its constituents could produce hepatoprotective, 2 antiviral 3 and antioxidative effects. 4 Recently, evidence from various animal and clinical studies has demonstrated that Radix Astragali may possess anticarcinogenic property, 5 which could attenuate the systemic side effects of conventional antineoplatic drugs. 6 Nonsteroidal anti-inflammatory drug (NSAID)-activated gene (NAG-1) is a novel therapeutic target which contributes to the antitumorigenic and proapoptotic effects in various cancer cells.…”

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confidence: 99%

A novel anticancer effect of Astragalus saponins: Transcriptional activation of NSAID‐activated gene

Auyeung

Cho

2009

Intl Journal of Cancer

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Astragalus membranaceus has been used to ameliorate the side effects of antineoplastic drugs because of its immunomodulating nature. We had recently demonstrated that total Astragalus saponins (AST) possess anticarcinogenic and proapoptotic properties in human colon cancer cells and tumor xenograft. In this study, we identified NSAID-activated gene (NAG-1) as a potential molecular target of AST. The growth-inhibitory and proapoptotic effects of AST were assessed in a panel of human cancer cell lines. Hoechst 33342 nuclear staining, Annexin V-FITC/propidium iodide staining, Western immunoblotting, real-time PCR, luciferase reporter assay and electrophoretic mobility shift assay were conducted to determine the association of NAG-1 and related transcription factors with AST during its regulation of apoptotic activities. Moreover, the combined proapoptotic and NAG-1 promoting activities of AST and/or inhibitors of the PI3K-Akt pathway were also examined. AST caused overexpression of NAG-1, leading to PARP cleavage and apoptosis. The induction of NAG-1 promoter activity by the drug was associated with increased gene expression, in addition to prior increase in Egr-1 expression and DNA binding activity. AST-induced NAG-1 activation was intensified when PI3K inhibitor LY294002 or Akt inhibitor was cotreated and reversed by NAG-1 siRNA transfection. Nevertheless, the extent of NAG-1 induction could not be altered by the ERK inhibitor PD98059. Our results indicate that NAG-1 is a potential molecular target of AST in its antitumorigenic and proapoptotic actions, which would have additive effects when used along with PI3K-Akt inhibitors. The information obtained could facilitate future development of a novel target-specific chemotherapeutic agent with known molecular pathway. ' 2009 UICC

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“…Different conditions in humans (1-3, 9, 13, 14) and experimental animals like rabbits (15), dogs (4, 6, 16), pigs (2, 17) and rats (5, 11) confirm the ESW injury on the renal parenchyma. Some factors can Table 1 produce contradictory results, including the sample size, different equipment with different energy principles, the number of impulses and the focus size (6).…”

Section: Commentsmentioning

confidence: 90%

Effects of repeated extracorporeal shock wave on kidney apoptosis of normal and diabetic rat

et al. 2008

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Objective: To assess the effect of repeated extracorporeal shock waves (ESW) on the apoptosis of renal parenchyma in normal and diabetic rats by the caspase-3 expression. Materials and methods: 80 rats were assigned for ESW (Direx Tripter X1® -14 KVA) to one of three groups with 50% rats with diabetes (A) and 50% normal (B) in each: G1 (n = 40): no ESW; G2 (n = 20): one ESW; G3 (n = 20): two ESWin an interval of 14 days. The animals were sacrificed 3 days after the ESW and samples of the renal parenchyma were histologically prepared, stained by the caspase-3 cleavage immunohistochemical method, and the apoptotic index (ApIn) was calculated by computer program Image Pro-Plus V 4,5,1 Media Cybernetics® ( ApIn = number of apoptotic cells in the microscopic section / total cells in the microscopic section). Results: There was no statistical difference in ApIn of normal rats with one ESW (8.4 ± 0.2) or two ESW (9.1 ± 0.3) and both were similar to the control group with no ESW (7.9 ± 0.2). The ApIn of diabetic rats with one ESW (7.4 ± 0.3) was similar to the normal rats with one or two ESW. On the other hand, the ApIn was statistically higher in diabetic rats with two ESW (11.9 ± 0.4) than in diabetic rats with one ESW (7.4 ± 0.3). Conclusions: The ApIn of normal rats with a single (2,000 SW) or repeated ESW (4,000 SW) was similar to the control animals without any ESW. In diabetic rats, the repeated ESW (4,000 SW) may result in an additional risk of tissue damage expressed by the higher ApIn.

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A novel antioxidant agent, astragalosides, prevents shock wave-induced renal oxidative injury in rabbits

Cited by 41 publications

References 30 publications

Biocatalysis of Cycloastragenol by Filamentous Fungi to Produce Unexpected Triterpenes

Biocatalysis of Cycloastragenol by Filamentous Fungi to Produce Unexpected Triterpenes

A novel anticancer effect of Astragalus saponins: Transcriptional activation of NSAID‐activated gene

Effects of repeated extracorporeal shock wave on kidney apoptosis of normal and diabetic rat

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