Objective: To assess the effect of repeated extracorporeal shock waves (ESW) on the apoptosis of renal parenchyma in normal and diabetic rats by the caspase-3 expression. Materials and methods: 80 rats were assigned for ESW (Direx Tripter X1® -14 KVA) to one of three groups with 50% rats with diabetes (A) and 50% normal (B) in each: G1 (n = 40): no ESW; G2 (n = 20): one ESW; G3 (n = 20): two ESWin an interval of 14 days. The animals were sacrificed 3 days after the ESW and samples of the renal parenchyma were histologically prepared, stained by the caspase-3 cleavage immunohistochemical method, and the apoptotic index (ApIn) was calculated by computer program Image Pro-Plus V 4,5,1 Media Cybernetics® ( ApIn = number of apoptotic cells in the microscopic section / total cells in the microscopic section). Results: There was no statistical difference in ApIn of normal rats with one ESW (8.4 ± 0.2) or two ESW (9.1 ± 0.3) and both were similar to the control group with no ESW (7.9 ± 0.2). The ApIn of diabetic rats with one ESW (7.4 ± 0.3) was similar to the normal rats with one or two ESW. On the other hand, the ApIn was statistically higher in diabetic rats with two ESW (11.9 ± 0.4) than in diabetic rats with one ESW (7.4 ± 0.3). Conclusions: The ApIn of normal rats with a single (2,000 SW) or repeated ESW (4,000 SW) was similar to the control animals without any ESW. In diabetic rats, the repeated ESW (4,000 SW) may result in an additional risk of tissue damage expressed by the higher ApIn.
The repeated extracorporeal shock waves and the renal parenchyma injury on normal and diabetic ratsThe repeated extracorporeal shock waves and the renal parenchyma injury on normal and diabetic rats ABSTRACT Purpose:To assess the effect of repeated extracorporeal shock waves (ESW) on renal parenchyma of normal and diabetic rats. Methods: 40 normal rats (A) and 40 diabetic rats (B) were assigned for ESW (Direx Tripter X1 ® -14 KVA) as follow: A1/B1 and A3/B3 no ESW; A2/B2 one ESW (2,000 SW); A4/B4 two ESW (4,000 SW) in an elapsed 14 days. All the animals were sacrificed 3 days after the ESW and samples of renal parenchyma were histological prepared, stained by H&E. For each animal the frequency of hemorrhage focus (HF) in the subcapasular, interstitial and glomerulus area was calculated (porcentage) on 20 randomly histological sections. Results: No one HF was identified in all normal or diabetic animals without ESW (A1, A3 and B1, B3). In the normal rats the HF frequency was similar to one ESW (subcapsular =15%; interstitial =20% and glomerular =10%) or repetead ESW (subcapsular =25%; interstitial =20%; glomerular=10%). In diabetic rats the occurence of HF with repetead ESW was more frequent (subcapsular =40%; interstitial =30% and glomerular =10%) than with a single ESW (subcapsular =25%; interstitial =15% and glomerular =15%). Conclusion: A single ESW or a repeated ESW caused a mild and similar damage on renal cortex of normal rats. In diabetic rats the repetead ESW may result in an accumulated damage, especially with focus of hemorrhage in subcapsular and interstitial tissue and glomerulus edema. Key words: Kidney. Lithotripsy. High-Energy Shock Waves. Diabetes Mellitus. Rats. RESUMOObjetivo: Avaliar o efeito de repetidas ondas de choque extracorpóreas (OCE) sobre o parênquima renal de ratos normais e diabéticos. Métodos: 40 ratos normais e 40 ratos diabéticos foram distribuídos para aplicação de OCE (Direx Tripter X1 ® -14 KVA) como segue: A1/B1 e A3/B3 sem OCE; A2/B2 uma sessão de OCE (2000 OC); A4/B4 duas sessões de OC (4000 OC) num intervalo de 14 dias. Todos os animais foram sacrificados no 3º. dia após a aplicação da OCE e amostras de parênquima renal foram histologicamente preparados e corados em H&E. Para cada animal foi calculado, em 20 campos aleatórios, a freqüência (em porcentagem) de focos hemorrágicos (FH) nas áreas subcapsular, intersticial e glomerular. Resultados: Nenhum foco hemorrágico foi identificado nos animais normais ou diabéticos que não receberam nenhuma OCE (A1, A3 e B1, B3). Nos ratos normais a freqüência de FH foi similar com uma sessão de OCE (subcapsular =15%; intersticial =20% e glomerular =10%) ou duas sessões de OCE (subcapsular =25%; intersticial =20%; glomerular =10%). Nos ratos diabéticos a ocorrência de FH com duas sessões de OCE foi mais freqüente (subcapsular =40%; intersticial =30% e glomerular =10%) do que com uma simples sessão de OCE (subcapsular =25%; intersticial =15% e glomerular =15%). Conclusão: Uma única ou duas sessões de OCE causa um discreto e semelhante d...
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