A novel anti-tumor agent, polyoxomolybdate induces apoptotic cell death in AsPC-1 human pancreatic cancer cells

Ogata, Aya; Mitsui, Shun; Yanagië, Hironobu; Kasano, H.; Hisa, Tomoyuki; Yamase, Toshihiro; Eriguchi, Masazumi

doi:10.1016/j.biopha.2004.11.008

Cited by 73 publications

(48 citation statements)

References 23 publications

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“…[NH 3 Pr i ] 6 [Mo 7 O 24 ] Á 3H 2 O (PM-8) was found to exhibit potent antitumour activity against MX-1 human breast cancer, Meth-A sarcoma, and MM46 adenocarcinoma in vivo (Yamase et al, 1988). Recent work from our laboratory showed that the efficacy of PM-8 has been recognised also for both AsPC-1 human pancreatic cancer and MKN45 human gastric cancer, which results from activation of the apoptotic pathway (Ogata et al, 2005;Mitsui et al, 2006;Yanagie et al, 2006). It has been proposed that PM-8 could be preferentially taken into tumour cells and converted into an approximately 10-fold more toxic species, produced through biological reduction probably in the mitochondrial system.…”

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confidence: 99%

Antitumour effect of polyoxomolybdates: induction of apoptotic cell death and autophagy in in vitro and in vivo models

et al. 2007

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O. The effect of PM-17 on the growth of cancer cell lines and xenografts was assessed by a cell viability test and analysis of tumour expansion rate. Morphological analysis was carried out by Hoechst staining, flow-cytometric analysis of Annexin V staining, terminal deoxynucleotidyl transferase-mediated 'nick-end' labelling staining, and electron-microscopic analysis. Activation of autophagy was detected by western blotting and fluorescence-microscopic analysis of the localisation of GFP-LC3 in transfected tumour cells. PM-17 inhibited the growth of human pancreatic cancer (AsPC-1) xenografts in a nude mice model, and induced morphological alterations in tumour cells. Correspondingly, PM-17 repressed the proliferation of AsPC-1 cells and human gastric cancer cells (MKN45) depending on the dose in vitro. We observed apoptotic patterns as the formation of apoptotic small bodies and translocation of phosphatidylserine by Hoechst staining and flow-cytometric analysis following Annexin V staining, and in parallel, autophagic conformation by the formulation of autophagosomes and localisation of GFP-LC3 by electron-and fluorescence-microscopic analysis.

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confidence: 99%

Antitumour effect of polyoxomolybdates: induction of apoptotic cell death and autophagy in in vitro and in vivo models

et al. 2007

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“…Two major elements in the Ag 2−x (NH 4 ) x Mo 3 O 10 ·3H 2 O nanowires are expected to have the antibacterial nature, that is, the Ag + ions and the Mo 6+ ions of the composite, both of which have been reported previously [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][41][42][43]. In this work, we have also observed a large amount of Ag-rich nanoparticles on surface of the nanowires.…”

Section: Resultsmentioning

confidence: 99%

Dissolvable Trimolybdate Nanowires as Ag Carriers for High-Efficiency Antimicrobial Applications

Xue

Jiang

et al. 2012

ISRN Nanotechnology

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Elimination of bacteria and other microbes effectively is important to our daily life and a variety of medical applications. Here, we introduce a new kind of trimolybdate nanowires, namelyAg2−x(NH4)xMo3O10⋅3H2O, that carry a large amount of Ag atoms in the lattice and Ag-rich nanoparticles on the surface. These nanowires can eliminate bacteria ofE. coli,Staphylococcus aureus, and unknown microbes in raw natural water with high efficiency. For example, they can inactivate more than 98% ofE. coliwith a nanowire concentration of only 5 ppm in the solution. The excellent sterilization performance is attributed to the combined effects of Ag ions, Mo ions, and Ag-rich nanoparticles of theAg2−x(NH4)xMo3O10⋅3H2Onanowires. These nanowires are not dissolvable in deionized water but can be dissolved by the metabolic materials released from bacteria, making them attractive for many biological applications.

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“…[1][2][3][4] In recent years, the applications of POMs in medicine have attracted particular interest. [5][6][7][8][9][10] POMs have been found to exhibit potential antiviral activity against many viruses including the notorious HIV. [5,6] Moreover, some POMs are reported to have prominent antitumor activity.…”

Section: Introductionmentioning

confidence: 99%

“…All these findings have resulted in an increasing number of relevant studies and very good progress has been made. [7][8][9] Nonetheless, the poor stability of POMs in water at physiological pH and the direct toxicity of some POMs still represent serious concerns in real clinical applications. [6,10] As a consequence, how to address these problems is of great significance for the practical use of POMs as pharmacologically active agents, especially as anticancer drugs.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Antitumoral Activity of Gelatin/Polyoxometalate Hybrid Nanoparticles

Guo

Cheng

et al. 2011

Macromolecular Bioscience

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Surfactant-free gelatin/heptamolybdate (HM) hybrid nanoparticles are prepared by a simple and environmentally friendly approach utilizing the electrostatic interaction between anionic HM and the zwitterionic gelatin. The obtained nanoparticles have a tunable size and very high HM loading content up to about 70%. In vitro and in vivo experiments prove that the gelatin/HM hybrid nanoparticles exhibit significantly better antitumor activity than plain ammonium heptamolybdate solution. Therefore, the gelatin/HM hybrid nanoparticles reported here may serve as a prototype platform for polymer/polyoxometalate (POM) hybrid nanoparticles as cancer treatment agents and hence open up more opportunities to maximize the potential of POM-based pharmaceutical agents.

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A novel anti-tumor agent, polyoxomolybdate induces apoptotic cell death in AsPC-1 human pancreatic cancer cells

Cited by 73 publications

References 23 publications

Antitumour effect of polyoxomolybdates: induction of apoptotic cell death and autophagy in in vitro and in vivo models

Antitumour effect of polyoxomolybdates: induction of apoptotic cell death and autophagy in in vitro and in vivo models

Dissolvable Trimolybdate Nanowires as Ag Carriers for High-Efficiency Antimicrobial Applications

Synthesis and Antitumoral Activity of Gelatin/Polyoxometalate Hybrid Nanoparticles

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