A non-enzymatic two step catalytic reduction of methylglyoxal by nanostructured V 2 O 5 modified electrode

Bhat, Lakshmishri Ramachandra; Vedantham, Srinivasan; Krishnan, Uma Maheswari

doi:10.1016/j.bios.2017.12.036

Cited by 18 publications

(5 citation statements)

References 42 publications

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“…Upon addition of GSH to Pt/rGMnO, a 2.3‐fold decrease in K s is observed. This substantiates the fact that GSH loses its electrons to MnO 2 (formed upon the oxidation of MnO) to get oxidized into GSSG resulting in a lower electron transfer rate [30]. The surface coverage was the least in Pt/rGMnO (0.488 μMcm −2 ) when compared to the bare electrode.…”

Section: Resultssupporting

confidence: 71%

“…The surface coverage was the least in Pt/rGMnO (0.488 μMcm À 2 ) when compared to the bare electrode. However, the lower Γ value of Pt/rGMnO could be attributed to the conversion of MnO 2 to MnO which aids the oxidation of GSH to GSSG thus yielding the inactivation of active sites present before the addition of GSH [30].…”

Section: Preliminary Electrochemical Studiesmentioning

confidence: 99%

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Design and Development of a Non‐Enzymatic Electrochemical Biosensor for the Detection of Glutathione

et al. 2022

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Glutathione (GSH-reduced form) is a tripeptide that plays a vital role as an antioxidant to remove xenobiotics in the human body and changes in GSH levels are a marker for the progression of various diseases. In this context, a highly sensitive non-enzymatic electrochemical biosensor for the detection of GSH has been developed using reduced graphene oxide Manganese oxide (rGMnO) nanocomposite as the nano-interface. Initially, graphene oxide was synthesized by Hummer's method and then thermally reduced in the presence of MnO 2 in a blast furnace to obtain rGMnO nanocompo-site. The nanocomposite was characterized to validate its structure and morphological properties via Scanning electron microscopy (SEM), X-ray diffraction (XRD), Raman, and X-ray photoelectron spectroscopy (XPS). Cyclic voltammetry and amperometry studies showed that upon the addition of GSH, the Pt/rGMnO modified working electrode exhibited a linear response in the range of 1-100 μM at an input voltage of À 0.62 V. The developed sensor was found to have a sensitivity of 0.3256 μA μM À 1 and LOD of 970 nM with a recovery of 92-104 % in real blood serum samples.

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Section: Resultssupporting

confidence: 71%

Section: Preliminary Electrochemical Studiesmentioning

confidence: 99%

Design and Development of a Non‐Enzymatic Electrochemical Biosensor for the Detection of Glutathione

et al. 2022

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“…Besides, previous studies found that higher MGO levels were observed in the plasma of patients with diabetes, and the plasma concentration was higher than 600 n m , indicating that this method is fully capable of testing requirements. Moreover, the entire detection procedure can be completed within 1 h, and the complex electrode modification [17] and nanomaterial preparation process [20] are not required, which can greatly improve the efficiency of actual detection.…”

Section: Resultsmentioning

confidence: 99%

“…Previously reported methods for determining MGO levels include chromatography,[ 14 , 15 , 16 ] electrochemistry, [17] fluorescent probes,[ 18 , 19 ] and colorimetry. [20] Yet, most of the methods are only suitable for the evaluation of in vitro serum samples, which limits the understanding of its pathological and physiological significance at the cellular and living level.…”

Section: Introductionmentioning

confidence: 99%

A Ratiometric Fluorescence Probe for Selective Detection of ex vivo Methylglyoxal in Diabetic Mice

et al. 2022

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Accurate monitoring of methylglyoxal (MGO) at cell and living level was crucial to reveal its role in the pathogenesis of diabetes since MGO was closely related to diabetes. Herein, a ratiometric fluorescence strategy was constructed based on the capture probe 2,3‐diaminonaphthalene (DAN) for the specific detection of MGO. Compared to the fluorescent probes with a single emission wavelength, the ratiometric mode by monitoring two emissions can effectively avoid the interference from the biological background, and provided additional self‐calibration ability, which can realize accurate detection of MGO. The proposed method showed a good linear relationship in the range of 0–75 μ m for MGO detection, and the limit of detection was 0.33 μ m . DAN responded to MGO with good specificity and was successfully applied for detecting the ex vivo MGO level in plasma of KK−Ay mice as a type II diabetes model. Besides, the prepared DAN test strip can be visualized for rapid semi‐quantitative analysis of MGO using the naked eye. Furthermore, human skin fibroblasts and HeLa cells were utilized for exogenous MGO imaging, and ex vivo MGO imaging was performed on tissues of KK−Ay mice. All results indicated that the DAN‐based ratiometric fluorescence probe can be used as a potential method to detect the level of MGO, thus enabling indications for the occurrence of diabetes and its complications.

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“…14,15 Among transition-metal oxides, vanadium pentoxide (V 2 O 5 ) has been acquired extensive researches because of its unique electrochromic properties and excellent electrocatalytic activity. 16,17 V 2 O 5 has received considerable attention in various fields including Li-ion batteries, 18 supercapacitors, 19 catalysts, 20 electrochromic devices, 21 and sensors [22][23][24] due to their outstanding properties. The chemical and physical properties of the V 2 O 5 can be improved through nanostructuring and surface modifications.…”

mentioning

confidence: 99%

Non-Enzymatic Amperometric Sensing of Hydrogen Peroxide Based on Vanadium Pentoxide Nanostructures

Ghanei-Motlagh

Taher

Fayazi

et al. 2019

J. Electrochem. Soc.

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Herein, a simple and selective electrochemical sensor was proposed for non-enzymatic determination of hydrogen peroxide (H 2 O 2 ). This sensor was fabricated by incorporation of the novel nanostructured orthorhombic vanadium pentoxide (V 2 O 5 ) into the carbon paste electrode (CPE) which provides significant catalytic activities for H 2 O 2 reduction. The electrochemical impedance spectroscopy (EIS) studies illustrated lower charge transfer resistance (R ct ) of the V 2 O 5 modified CPE compared to the unmodified CPE. The effects of various experimental factors such as solution pH, applied potential and amount of modifier were studied in an amperometric mode. After optimization, the proposed method displayed a wide linear detection range from 5.0 to 1400.0 μM with a low detection limit of 2.5 μM based S/N = 3 and a response time less than 5 s. The sensitivity of 3.44 μA μM −1 cm −2 was acquired in the present method for H 2 O 2 quantification is considerably better than other reported amperometric sensors with similar detection limits. In addition, the designed sensor depicted good reproducibility, remarkable selectivity, and excellent stability. The modified CPE was applicable for analysis of H 2 O 2 in some cosmetic and personal care products.

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A non-enzymatic two step catalytic reduction of methylglyoxal by nanostructured V 2 O 5 modified electrode

Cited by 18 publications

References 42 publications

Design and Development of a Non‐Enzymatic Electrochemical Biosensor for the Detection of Glutathione

Design and Development of a Non‐Enzymatic Electrochemical Biosensor for the Detection of Glutathione

A Ratiometric Fluorescence Probe for Selective Detection of ex vivo Methylglyoxal in Diabetic Mice

Non-Enzymatic Amperometric Sensing of Hydrogen Peroxide Based on Vanadium Pentoxide Nanostructures

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