Design and Development of a Non‐Enzymatic Electrochemical Biosensor for the Detection of Glutathione

Abstract

Glutathione (GSH-reduced form) is a tripeptide that plays a vital role as an antioxidant to remove xenobiotics in the human body and changes in GSH levels are a marker for the progression of various diseases. In this context, a highly sensitive non-enzymatic electrochemical biosensor for the detection of GSH has been developed using reduced graphene oxide Manganese oxide (rGMnO) nanocomposite as the nano-interface. Initially, graphene oxide was synthesized by Hummer's method and then thermally reduced in the presence of MnO 2 in a blast furnace to obtain rGMnO nanocompo-site. The nanocomposite was characterized to validate its structure and morphological properties via Scanning electron microscopy (SEM), X-ray diffraction (XRD), Raman, and X-ray photoelectron spectroscopy (XPS). Cyclic voltammetry and amperometry studies showed that upon the addition of GSH, the Pt/rGMnO modified working electrode exhibited a linear response in the range of 1-100 μM at an input voltage of À 0.62 V. The developed sensor was found to have a sensitivity of 0.3256 μA μM À 1 and LOD of 970 nM with a recovery of 92-104 % in real blood serum samples.

Covalent Organic Frameworks-TpPa-1 as an Emerging Platform for Electrochemical Sensing

“…); Figure S17: Amperometric responses of PCs/CPE to successive additions of various concentrations of GSH spiked in serum in 0.1 M PBS (pH = 4.0) at 0.25 V; Figure S18: Amperometric responses of PCs/CPE for the detection of GSH spiked in human serum in 0.1 M PBS (pH = 4.0) at 0.25 V; Table S1: Comparison of the Pd 2+ /COFs/GCE for different parameters with previously reported hydrazine electrodes; Table S2: Determination of hydrazine spiked in water samples; Table S3: Comparison of different modified GCE for detection of ONP and PNP; Table S4; Comparison of different modified electrodes for electrochemical sensing of GSH; Table S5; Determination of GSH spiked in human serum by successive additions;Table S6; Determination of GSH spiked in human serum by a non-successive addition method. References [ 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 ] were cited in the Supplementary Materials.…”