A new way to fast and high resolution determination of modified nucleosides

Studzińska, Sylwia; Buszewski, Bogusław

doi:10.1016/j.jchromb.2012.01.017

Cited by 13 publications

(1 citation statement)

References 25 publications

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“…The use of C 18 involves a time‐consuming separation step (to avoid co‐elution of similar compounds) when multiple analyses of nucleosides in urine are performed (Dudley et al ., ; Xu et al ., ; Qian et al ., ). On the other hand there are a variety of stationary phases for RP‐HPLC and selectivity of separation may be improved when other columns are applied for the analysis of studied compounds (Kowalska et al ., ; Studzińska and Buszewski, ). Aminopropyl, alkylamide and cholesterol packing materials have been utilized for this purpose (Kowalska et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Analysis of normal and modified nucleosides in urine samples by high‐performance liquid chromatography with different stationary phases

Studzińska

Buszewski

2014

Biomedical Chromatography

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The main aim of the present work was to study the retention behavior and quantification of nine nucleosides with the use of octadecyl, alkylamide, cholesterol and alkyl-phosphate stationary phases. The influence of organic solvent and buffer concentration on the separation of these compounds was under investigation. The retention factor had the highest values for the octadecyl and cholesterol packing materials. Complete separation of all the studied nucleosides was achieved in case of cholesterol stationary phase. The optimized separation method was applied for the quantification of nucleosides in the urine samples. Calibration plots showed good linearity (R(2) > 0.999) and the limits of detection were in a range of 0.3-0.5 µg/mL, while the limits of quantitation were >0.9 µg/mL. Accuracy was in the range of 5-11%.

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