Hydrophilic interaction chromatography-type sorbent prepared by the modification of methacrylate-base resin with polyethyleneimine for solid-phase extraction of polar compounds

Qualitative and Quantitative Analytical Techniques of Nucleic Acid Modification Based on Mass Spectrometry for Biomarker Discovery

“…The purpose of nucleoside extraction is to improve separation and detection efficiency and obtain higher signals of the analytes by removing salts, proteins, and uninterested nucleosides, especially large amounts of normal nucleosides, from the matrix. The initial extraction methods enrich free nucleosides from biological samples such as serum and urine, normally based on hydrophobic or hydrophilic interactions, ion exchange, and affinity with 1,2-cis-diol compounds with commercial materials, such as HLB [ 64 , 65 ] and WCX [ 66 ], as well as new materials such as graphene [ 67 ], polymers [ 68 ], metal oxides [ 69 , 70 , 71 ], and boronate-decorated substrates [ 72 , 73 , 74 ], which are also used for the extraction of nucleoside products after enzymatic digestion to further increase signal response. The shapes of substrate and extraction devices influence extraction efficiency [ 75 , 76 ].…”

Enriching adenosine by thymine-rich DNA oligomers

“…25 Hydrophilic interaction chromatography (HILIC)-type sorbents are also used for Ado separation. 26 We herein aim to develop a simple enrichment and separation method that involves the easy preparation of the Ado sorbent. In this method the Ado-sorbent interaction is through a noncovalent reaction, and it works in a broad pH range ( pH 5.0-8.0).…”

“…25 Hydrophilic interaction chromatography (HILIC)-type sorbents are also used for Ado separation. 26…”