A new, very sensitive method of assessment of ptaquiloside, the major bracken carcinogen in the milk of farm animals

Bonadies, F.; Berardi, Giorgio; Nicoletti, Rosario; Romolo, Francesco Saverio; Francesco, De Giovanni; Marabelli, R.; Adriano, Santoro; Raso, Cinzia; Tagarelli, Antonio; Roperto, F.; Russo, Valeria; Roperto, Sante

doi:10.1016/j.foodchem.2010.05.059

Cited by 26 publications

(14 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sample preparation was carried out following a protocol reported elsewhere . The carbograph cartridge was washed with 6 mL of a mixture of acetonitrile/water (7:3, v/v); it was subsequently activated with diluted HCl (pH 2, 20 mL) and, finally, washed with 6 mL of water.…”

Section: Materials and Methodsmentioning

confidence: 99%

Ptaquiloside, the Major Carcinogen of Bracken Fern, in the Pooled Raw Milk of Healthy Sheep and Goats: An Underestimated, Global Concern of Food Safety

Virgilio

Sinisi

Russo

et al. 2015

J. Agric. Food Chem.

View full text Add to dashboard Cite

Bracken fern (Pteridium aquilinum) is a worldwide plant containing toxic substances, which represent an important chemical hazard for animals, including humans. Ptaquiloside, 1, a norsesquiterpenoid glucoside, is the major carcinogen of bracken detected in the food chain, particularly in the milk from farm animals. To date, ptaquiloside has been shown in the milk of cows feeding on a diet containing bracken fern. This is the first study that shows the systematic detection of ptaquiloside, 1, and reports its direct quantitation in pooled raw milk of healthy sheep and goats grazing on bracken. Ptaquiloside, 1, was detected by a sensitive method based on the chemical conversion of ptaquiloside, 1, into bromopterosine, 4, following gas chromatography-mass spectrometry (GC-MS) analysis. The presence of ptaquiloside, 1, possibly carcinogenic to humans, in the milk of healthy animals is an unknown potential health risk, thus representing a harmful and potential global concern of food safety.

show abstract

Section: Materials and Methodsmentioning

confidence: 99%

Ptaquiloside, the Major Carcinogen of Bracken Fern, in the Pooled Raw Milk of Healthy Sheep and Goats: An Underestimated, Global Concern of Food Safety

Virgilio

Sinisi

Russo

et al. 2015

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…In Brazil, only the last three syndromes have been reported in cattle [3][4][5]. In addition to poisoning the cattle, the consumption of the plant by these animals presents a potential risk for humans, since residues of ptaquiloside can be found in milk [6][7][8] and meat [9,10]. Several chemical compounds have been identified in Pteridium ferns, the main one being a norsesquiterpenoid glycoside called ptaquiloside, which has been implicated in cattle poisoning and human cancer [3,4].…”

Section: Introductionmentioning

confidence: 99%

Ptaquiloside and Pterosin B Levels in Mature Green Fronds and Sprouts of Pteridium arachnoideum

Ribeiro¹,

Keller

Soto-Blanco

2020

Toxins

View full text Add to dashboard Cite

Pteridium arachnoideum, a fern of the Pteridium aquilinum species complex found in South America, is responsible for several different syndromes of poisoning. Cases of bovine enzootic hematuria and upper alimentary squamous cell carcinoma are both frequent occurrences in Brazil, whereas only bovine enzootic hematuria is noted with any frequency around the world. The reason for the high frequency of upper alimentary squamous cell carcinoma in Brazil is not currently known. One possible explanation may be the higher levels of ptaquiloside and pterosin B in Brazilian Pteridium than those present in the plant in other countries. However, these levels have not yet been determined in P. arachnoideum. Thus, the present study aimed to measure and compare ptaquiloside and pterosin B levels in mature green fronds and sprouts of P. arachnoideum collected from different locations in Brazil. Samples of P. arachnoideum were collected from the states of Minas Gerais and Rio Grande do Sul. A total of 28 mature leaf samples and 23 sprout samples were used. The mean concentrations of ptaquiloside and pterosin B present in the mature green fronds of P. arachnoideum ranged from 2.49 to 2.75 mg/g and 0.68 to 0.88 mg/g, respectively; in P. arachnoideum sprouts, mean concentrations of ptaquiloside and pterosin B ranged from 12.47 to 18.81 mg/g, and 4.03 to 10.42 mg/g for ptaquiloside and pterosin B, respectively. Thus, ptaquiloside and pterosin B levels in P. arachnoideum samples collected in Brazil were higher in sprouts than in mature green fronds, as observed in other countries. However, there was no variation in ptaquiloside levels among plants collected from different cities in Brazil. The high frequency of upper alimentary squamous cell carcinoma in Brazilian cattle may not be attributed to greater levels of ptaquiloside and pterosin B in P. arachnoideum than in other Pteridium species in other countries.

show abstract

“…Plants of Pteridium aquilinum complex contain several active principles, the most toxicological being ptaquiloside, a norsesquiterpene glucoside, which under alkaline pH conditions gets converted into dienone, a potent DNA alkylating agent with carcinogenic activity (YAMADA et al, 2007). This compound can be secreted in milk (ALONSO-AMELOT et al, 1998;FRANCESCO et al, 2011) presenting a potential health risk to the consumers of contaminated milk. However, the frequency and the amount of ptaquiloside contamination in milk obtained from Brazilian cattle, has not yet been well elucidated.…”

Section: Introductionmentioning

confidence: 99%

“…Consumption of bracken ferns by lactating cows can result in the secretion of ptaquiloside, a carcinogen present in bracken ferns, into milk, which could have adverse effects on the consumers of this milk. In fact, milk from cows that either under experimental set-up (ALONSO-AMELOT et al, 1998) or spontaneously (FRANCESCO et al, 2011;ROSERO NEGRETE, 2013;ARANHA et al, 2014;TOBAR et al, 2014;VIRGILIO et al, 2015) ingested bracken ferns showed the presence of ptaquiloside as determined by different analytical methods. However, there are no reports on the presence of ptaquiloside in milk obtained from cows raised in Brazil.…”

Section: Introductionmentioning

confidence: 99%

“…For extraction method C, samples were treated with KOH to alkalinize the aqueous solution followed by heat treatment for converting ptaquiloside into pterosin B, as described previously (ALONSO-AMELOT et al, 1998;FRANCESCO et al, 2011;ROSERO NEGRETE, 2013;TOBAR et al, 2014). Subsequently, dichloromethane was used as a solvent for extraction followed by freezing and centrifugation to achieve efficient separation of fat and proteins.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of ptaquiloside in cow's milk by HPLC-UV

Ribeiro¹,

Keller

Melo

et al. 2019

SCA

View full text Add to dashboard Cite

Ferns of the genus Pteridium are known to be responsible for cases of poisoning in cattle. The commonly found species of Pteridium in Brazil are P. arachnoideum and P. caudatum. The toxic principle of these ferns is ptaquiloside, a norsesquiterpene glucoside, which is a known carcinogen. Ptaquiloside can be secreted in the milk of cows grazing on bracken ferns, thereby posing a potential health risk for the milk consumers. The objective of the present study was to develop an analytical methodology using high performance liquid chromatography (HPLC) with ultraviolet (UV) detection to determine the concentrations of ptaquiloside in cow´s milk. HPLC-UV method, based on the conversion of ptaquiloside into pterosin B and its subsequent extraction using dichloromethane, was successfully developed for estimating concentrations of ptaquiloside in milk samples. However, the presence of ptaquiloside was not detected in milk samples obtained from cows with a history of bovine enzootic hematuria resulting from grazing on P. arachnoideum infested pastures in Brazil.

show abstract

A new, very sensitive method of assessment of ptaquiloside, the major bracken carcinogen in the milk of farm animals

Cited by 26 publications

References 27 publications

Ptaquiloside, the Major Carcinogen of Bracken Fern, in the Pooled Raw Milk of Healthy Sheep and Goats: An Underestimated, Global Concern of Food Safety

Ptaquiloside, the Major Carcinogen of Bracken Fern, in the Pooled Raw Milk of Healthy Sheep and Goats: An Underestimated, Global Concern of Food Safety

Ptaquiloside and Pterosin B Levels in Mature Green Fronds and Sprouts of Pteridium arachnoideum

Determination of ptaquiloside in cow's milk by HPLC-UV

Contact Info

Product

Resources

About