A new vector, pGID052, for genetic transfer in

Beltramo, Charlotte; Oraby, Mona M.; Bourel, Gérald; Garmyn, Dominique; Guzzo, Jean

doi:10.1016/j.femsle.2004.05.029

Cited by 6 publications

(2 citation statements)

References 20 publications

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“…Sequence analyses of plasmids indicate that they carry two genes possibly involved in wine adaptation encoding a predicted sulphite exporter ( tau E ) and a NADH: flavin oxidoreductase [ 198 ]. Due to the important role of O. oeni in the process of wine-making, various attempts have been made to develop cloning vectors and transformation protocols for O. oeni based on these plasmids [ 192 , 193 , 194 , 195 , 196 , 197 , 198 , 199 , 200 ]. At the beginning, small RCR plasmids pRS1, pRS2 and pRS3 from O. oeni were used for development of vectors [ 196 , 200 ].…”

Section: Diversity and Similarity Of Plasmids From Labmentioning

confidence: 99%

Plasmids from Food Lactic Acid Bacteria: Diversity, Similarity, and New Developments

Cui

et al. 2015

IJMS

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Plasmids are widely distributed in different sources of lactic acid bacteria (LAB) as self-replicating extrachromosomal genetic materials, and have received considerable attention due to their close relationship with many important functions as well as some industrially relevant characteristics of the LAB species. They are interesting with regard to the development of food-grade cloning vectors. This review summarizes new developments in the area of lactic acid bacteria plasmids and aims to provide up to date information that can be used in related future research.

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Section: Diversity and Similarity Of Plasmids From Labmentioning

confidence: 99%

Plasmids from Food Lactic Acid Bacteria: Diversity, Similarity, and New Developments

Cui

et al. 2015

IJMS

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“…Oenococcus oeni seems to be good model for the study of mechanisms involved in stress resistance, but there are difficulties to genetically modify the O. oeni cells even using the current molecular biology technologies (Beltramo et al, 2004; Assad-Garcia et al, 2008; Darsonval et al, 2016). Currently, more attention was turned to other LABs as a model to explore O. oeni , Schümann et al (2012) expressed O. oeni mle in L. plantarum WCFS1, and the recombinant L. plantarum cells expressing MLE accelerate the malolactic fermentation.…”

Section: Introductionmentioning

confidence: 99%

Heterologous Expression of Argininosuccinate Synthase From Oenococcus oeni Enhances the Acid Resistance of Lactobacillus plantarum

et al. 2019

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Oenococcus oeni can survive well in wine (an acid-stress environment) and dominate malolactic fermentation (MLF). To demonstrate a possible role of argininosuccinate synthase gene ( argG ) in the acid tolerance response of O. oeni , a related argG gene was inserted into a plasmid pMG36e and heterologously expressed in Lactobacillus plantarum SL09, a wine isolate belonging to a species of relevant importance in MLF. The expression levels of the argG gene in L. plantarum were analyzed by RT-qPCR, argininosuccinate synthase (ASS) activity and cell properties (amino acids, pH, H + -ATPase activity, and ATP levels) were determined at pH 3.7 in comparison with that at pH 6.3. Results showed that the recombinant strain L. plantarum SL09 (pMG36e argG ) exhibited stronger growth performance compared with the control strain (without argG gene), and the expression levels of hsp1 , cfa , atp , the citrate and malate metabolic genes were apparently increased under acid stress. In addition, the recombinant strain exhibited 11.0-, 2.0-, 1.9-fold higher ASS activity, H + -ATPase activity and intracellular ATP level, compared with the corresponding values for control strain during acid-stresses condition, which may take responsible for the acid tolerance enhancement of the recombinant strain. This is the first work report on heterologous expression of argG gene, and the results presented in this study will be beneficial for the research on acid stress response of O. oeni.

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Characterization of a Cryptic Rolling-Circle Replication Plasmid pMK8 from Enterococcus durans 1–8

Zhou

Zhai

et al. 2018

Curr Microbiol

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A novel cryptic plasmid from Enterococcus durans 1-8, designated as pMK8, was sequenced and analyzed in this study. It consists of 3337 bp with a G + C content of 33.11%. Sequence analysis of pMK8 revealed three putative open reading frames (ORFs). Based on homology, two of them were identified as genes encoding replication initiation (RepC) and mobilization (Mob) protein, respectively. Sequence analysis revealed a pT181 family double-strand origin (dso) and a putative single-strand origin (sso) located upstream of the repC gene. Sequence homology analysis indicated that the sso belongs to the ssoW family. Southern hybridization confirmed the presence of single-strand DNA (ssDNA) intermediates, suggesting that pMK8 replicates via the RCR mechanism. Furthermore, the relative copy number of pMK8 was estimated by real-time PCR to be 175 ± 14 copies in each cell.

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A new vector, pGID052, for genetic transfer in

Cited by 6 publications

References 20 publications

Plasmids from Food Lactic Acid Bacteria: Diversity, Similarity, and New Developments

Plasmids from Food Lactic Acid Bacteria: Diversity, Similarity, and New Developments

Heterologous Expression of Argininosuccinate Synthase From Oenococcus oeni Enhances the Acid Resistance of Lactobacillus plantarum

Characterization of a Cryptic Rolling-Circle Replication Plasmid pMK8 from Enterococcus durans 1–8

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