“…For example, a recent study of the rat cecum meta-mobilome revealed hundreds of small novel cryptic plasmids ( Jørgensen et al, 2014 ). Such small cryptic plasmids may carry very few genes next to their replication machinery (e.g., Carneiro et al, 2016 ; Zhou et al, 2018 ). Plasmid persistence (i.e., the plasmid presence within a microbial population over time) in the absence of positive selection is mainly dependent on stable plasmid replication and segregation, that is, reproduction and vertical inheritance ( Bergstrom et al, 2000 ; Paulsson, 2002 ).…”