Heterologous Expression of Argininosuccinate Synthase From Oenococcus oeni Enhances the Acid Resistance of Lactobacillus plantarum

Abstract

Oenococcus oeni can survive well in wine (an acid-stress environment) and dominate malolactic fermentation (MLF). To demonstrate a possible role of argininosuccinate synthase gene ( argG ) in the acid tolerance response of O. oeni , a related argG gene was inserted into a plasmid pMG36e and heterologously expressed in Lactobacillus plantarum SL09, a wine isolate belonging to a species of relevant importance in MLF. The expression levels of the argG gene in L. plantarum were analyzed by RT-qPCR, argininosuccinate synthase (ASS) activity and cell properties (amino acids, pH, H + -ATPase activity, and ATP levels) were determined at pH 3.7 in comparison with that at pH 6.3. Results showed that the recombinant strain L. plantarum SL09 (pMG36e argG ) exhibited stronger growth performance compared with the control strain (without argG gene), and the expression levels of hsp1 , cfa , atp , the citrate and malate metabolic genes were apparently increased under acid stress. In addition, the recombinant strain exhibited 11.0-, 2.0-, 1.9-fold higher ASS activity, H + -ATPase activity and intracellular ATP level, compared with the corresponding values for control strain during acid-stresses condition, which may take responsible for the acid tolerance enhancement of the recombinant strain. This is the first work report on heterologous expression of argG gene, and the results presented in this study will be beneficial for the research on acid stress response of O. oeni.

Petunidin-Based Anthocyanin Relieves Oxygen Stress in Lactobacillus plantarum ST-III

“…L. plantarum is a lactic acid bacterium found in diverse ecological niches, highlighting its particular capabilities of adaptation ( Seddik et al, 2017 ). Therefore, it is employed as a model organism for academic research of lactic acid bacteria ( Siezen and van Hylckama Vlieg, 2011 ; Seddik et al, 2017 ; Parlindungan et al, 2019 ), also in the area of stress response and tolerance ( Fiocco et al, 2010 ; Papadimitriou et al, 2016 ; Arena et al, 2019 ; Zhao et al, 2019 ). Studies on L. plantarum have deepened the understanding of oxygen-tolerance in Lactobacillus and other probiotics ( Archibald and Fridovich, 1981 ; Serrano et al, 2007 ; Papadimitriou et al, 2016 ).…”

The local transcriptional regulators SacR1 and SacR2 act as repressors of fructooligosaccharides metabolism in Lactobacillus plantarum

“…Lactobacillus plantarum is a Gram-positive bacterium that resides naturally in the human gastrointestinal tract (GIT) [ 1 , 2 ]. This species is a common and versatile type of lactic acid bacteria (LAB) used in the production of several fermented and functional foods [ 1 , 3 – 5 ].…”

“…Lactobacillus plantarum is a Gram-positive bacterium that resides naturally in the human gastrointestinal tract (GIT) [ 1 , 2 ]. This species is a common and versatile type of lactic acid bacteria (LAB) used in the production of several fermented and functional foods [ 1 , 3 – 5 ]. Like most lactobacilli, L. plantarum strains have complex nutritional requirements for fermentable carbohydrates and can utilize a wide range of carbohydrates, including some prebiotics [ 4 , 6 , 7 ].…”

“…Several studies have demonstrated that L. plantarum can effectively utilize FOS [ 10 – 12 ]. This advantage helps the survival and colonization of L. plantarum in the GIT [ 1 , 6 , 8 ].…”

The local transcriptional regulators SacR1 and SacR2 act as repressors of fructooligosaccharides metabolism in Lactobacillus plantarum

“…Lactobacillus plantarum is a Gram-positive bacterium that resides naturally in the human gastrointestinal tract (GIT) [1,2]. This species is a common and versatile type of lactic acid bacteria (LAB) used in the production of several fermented and functional foods [1,[3][4][5]. Like most lactobacilli, L. plantarum strains have complex nutritional requirements for fermentable carbohydrates and can utilize a wide range of carbohydrates, including some prebiotics [4,6,7].…”