A new strategy for simultaneous synthesis and efficient anchorage of polymer monoliths in native PDMS microchips

Araya-Farias, Monica; Taverna, Myriam; Woytasik, Marion; Bayle, Fabien; Guerrouache, Mohamed; Ayed, Ichraf; Cao, Hong; Carbonnier, Benjamin; Tran, Nguyet Thuy

doi:10.1016/j.polymer.2015.04.039

Cited by 15 publications

(21 citation statements)

References 42 publications

(38 reference statements)

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“…Several methods of porous monolith fabrication inside PDMS microfluidics [21][22][23][24] have been published, and in particular, Araya-Farias et al 24 developed an innovative single step approach-a monolith consisting of ethylene glycol methacrylate phosphate monomer and N,N 0 -methylene-bis-acrylamide crosslinker.…”

Section: Monolith Fabricationmentioning

confidence: 99%

Porous monolith microfluidics for bacterial cell-to-cell communication assays

et al. 2017

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Genetically engineered bacteria can be used for a wide range of applications, from monitoring environmental toxins to studying complex communication networks in the human digestive system. Although great strides have been made in studying single strains of bacteria in well-controlled microfluidic environments, there remains a need for tools to reliably control and measure communication between multiple discrete bacterial populations. Stable long-term experiments (e.g., days) with controlled population sizes and regulated input (e.g., concentration) and output measurements can reveal fundamental limits of cell-to-cell communication. In this work, we developed a microfluidic platform that utilizes a porous monolith to reliably and stably partition adjacent strains of bacteria while allowing molecular communication between them for several days. We measured small molecule production by the bacterial populations in response to stimuli using analytical chemistry methods and measured fluorescent output. The results are compared with communication and diffusion delay models. This porous monolith microfluidic system enables bacterial cell-to-cell communication assays with dynamic control of inputs, relatively long-term experimentation with no cross contamination, and stable bacterial population size. This system can serve as a valuable tool in understanding bacterial communication and improving biosensor design capabilities.

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Section: Monolith Fabricationmentioning

confidence: 99%

Porous monolith microfluidics for bacterial cell-to-cell communication assays

et al. 2017

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“…However, such a procedure could not be readily used in much smaller channels as they could be blocked during swelling. A single-step approach for monolith anchoring was introduced by Araya-Farias et al 27 who studied several photoinitiators for preparing a monolith from a novel monomer, ethylene glycol methacrylate phosphate. It was found that a high concentration of photoinitiator, in this case 2-methyl-4 0 -(methylthio)-2-morpholino-propiophenone, provided efficient hydrogen abstraction from the channel surface resulting in a robust anchoring of the monolith to the PDMS wall.…”

Section: Materials Development a Organic Porous Polymersmentioning

confidence: 99%

Advances in monoliths and related porous materials for microfluidics

2016

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In recent years, the use of monolithic porous polymers has seen significant growth. These materials present a highly useful support for various analytical and biochemical applications. Since their introduction, various approaches have been introduced to produce monoliths in a broad range of materials. Simple preparation has enabled their easy implementation in microchannels, extending the range of applications where microfluidics can be successfully utilized. This review summarizes progress regarding monoliths and related porous materials in the field of microfluidics between 2010 and 2015. Recent developments in monolith preparation, solid-phase extraction, separations, and catalysis are critically discussed. Finally, a brief overview of the use of these porous materials for analysis of subcellular and larger structures is given.

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“…The integration of methacrylate and other monolithic materials into microfluidic devices has benefited both analyte separation and pre-concentration in modular microfluidics as the quest for the miniaturization of chromatographic devices continues [41,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70]. …”

Section: Methacrylate Polymer Monolithsmentioning

confidence: 99%

“…Methacrylate monoliths have also been fabricated within microfluidic devices or channels; for example, in polymer High Internal Phase Emulsion (polyHIPE) separation columns [ 53 ] and in monolithic pre-concentration devices [ 54 , 55 ]. The integration of methacrylate and other monolithic materials into microfluidic devices has benefited both analyte separation and pre-concentration in modular microfluidics as the quest for the miniaturization of chromatographic devices continues [ 41 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 ].…”

Section: Methacrylate Polymer Monolithsmentioning

confidence: 99%

Methacrylate Polymer Monoliths for Separation Applications

Groarke

Brabazon

2016

Materials

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This review summarizes the development of methacrylate-based polymer monoliths for separation science applications. An introduction to monoliths is presented, followed by the preparation methods and characteristics specific to methacrylate monoliths. Both traditional chemical based syntheses and emerging additive manufacturing methods are presented along with an analysis of the different types of functional groups, which have been utilized with methacrylate monoliths. The role of methacrylate based porous materials in separation science in industrially important chemical and biological separations are discussed, with particular attention given to the most recent developments and challenges associated with these materials. While these monoliths have been shown to be useful for a wide variety of applications, there is still scope for exerting better control over the porous architectures and chemistries obtained from the different fabrication routes. Conclusions regarding this previous work are drawn and an outlook towards future challenges and potential developments in this vibrant research area are presented. Discussed in particular are the potential of additive manufacturing for the preparation of monolithic structures with pre-defined multi-scale porous morphologies and for the optimization of surface reactive chemistries.

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A new strategy for simultaneous synthesis and efficient anchorage of polymer monoliths in native PDMS microchips

Cited by 15 publications

References 42 publications

Porous monolith microfluidics for bacterial cell-to-cell communication assays

Porous monolith microfluidics for bacterial cell-to-cell communication assays

Advances in monoliths and related porous materials for microfluidics

Methacrylate Polymer Monoliths for Separation Applications

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