Advances in monoliths and related porous materials for microfluidics

Knob, Radim; Sahore, Vishal; Sonker, Mukul

doi:10.1063/1.4948507

Cited by 34 publications

(29 citation statements)

References 104 publications

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“…These materials are easily prepared in-situ by photoinitiated polymerization, and their surface and porous properties can be tuned by monomers or porogens in the polymerization mixture. Batch-to-batch variability with free-radical polymerization is less of an issue for sample preparation than for high-performance separations [24]. Thus we prepared a porous polymer modified with a capture probe for selective nucleic acid capture.…”

Section: Resultsmentioning

confidence: 99%

Sequence-specific DNA solid-phase extraction in an on-chip monolith: Towards detection of antibiotic resistance genes

Knob

Nelson

Robison

2017

Journal of Chromatography A

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Antibiotic resistance of bacteria is a growing problem and presents a challenge for prompt treatment in patients with sepsis. Currently used methods rely on culturing or amplification; however, these steps are either time consuming or suffer from interference issues. A microfluidic device was made from black polypropylene, with a monolithic column modified with a capture oligonucleotide for sequence selective solid-phase extraction of a complementary target from a lysate sample. Porous properties of the monolith allow flow and hybridization of a target complementary to the probe immobilized on the column surface. Good flow through properties enable extraction of a 100 μL sample and elution of target DNA in 12 minutes total time. Using a fluorescently labeled target oligonucleotide related to Verona Integron-Mediated Metallo-β-lactamase it was possible to extract and detect a 1 pM sample with 83% recovery. Temperature-mediated elution by heating above the duplex melting point provides a clean extract without any agents that interfere with base pairing, allowing various labeling methods or further downstream processing of the eluent. Further integration of this extraction module with a system for isolation and lysis of bacteria from blood, as well as combining with single-molecule detection should allow rapid determination of antibiotic resistance.

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Section: Resultsmentioning

confidence: 99%

Sequence-specific DNA solid-phase extraction in an on-chip monolith: Towards detection of antibiotic resistance genes

Knob

Nelson

Robison

2017

Journal of Chromatography A

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“…Antibodies can be placed in a microfluidic setup by device surface modification [11, 12] or through a solid support like porous polymer monoliths [13], beads [14, 15] or nanoparticles [16, 17] introduced into microchannels.…”

Section: On-chip Sample Preparation Methodsmentioning

confidence: 99%

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

Sonker

Sahore

Woolley

2017

Analytica Chimica Acta

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138

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Microfluidics is a vibrant and expanding field that has the potential for solving many analytical challenges. Microfluidics show promise to provide rapid, inexpensive, efficient, and portable diagnostic solutions that can be used in resource-limited settings. Researchers have recently reported various microfluidic platforms for biomarker analysis applications. Sample preparation processes like purification, preconcentration and labeling have been characterized on-chip. Additionally, improvements in microfluidic separation techniques have been reported for cellular and molecular biomarkers. This review critically evaluates microfluidic sample preparation platforms and separation methods for biomarker analysis reported in the last two years. Key advances in device operation and ability to process different sample matrices in a variety of device materials are highlighted. Finally, current needs and potential future directions for microfluidic device development to realize its full diagnostic potential are discussed.

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“…18, 19 Solid supports can be made using packed materials, 18, 20 monoliths, 21, 22 hydrogels, 23, 24 or membranes. 6, 25 First introduced in microfluidics by Fréchet et al, 21 monoliths have been used extensively for SPE, preconcentration and sample modification 26, 27 due to their facile fabrication, low backpressure and surface modification capabilities. 27, 28 Monoliths used for SPE in microfluidics include affinity 13, 14, 20, 29 and reversed-phase.…”

Section: Introductionmentioning

confidence: 99%

“…6, 25 First introduced in microfluidics by Fréchet et al, 21 monoliths have been used extensively for SPE, preconcentration and sample modification 26, 27 due to their facile fabrication, low backpressure and surface modification capabilities. 27, 28 Monoliths used for SPE in microfluidics include affinity 13, 14, 20, 29 and reversed-phase. 11, 15, 30 Reversed-phase monoliths are used to retain analytes based on hydrophobic interactions, allowing preconcentration or separation.…”

Section: Introductionmentioning

confidence: 99%

On-chip fluorescent labeling using reversed-phase monoliths and microchip electrophoretic separations of selected preterm birth biomarkers

et al. 2016

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On-chip preconcentration, purification, and fluorescent labeling are desirable sample preparation steps to achieve complete automation in integrated microfluidic systems. In this work, we developed electrokinetically operated microfluidic devices for solid-phase extraction and fluorescent labeling of preterm birth (PTB) biomarkers. Reversed-phase monoliths based on different acrylate monomers were photopolymerized in cyclic olefin copolymer microdevices and studied for the selective retention and elution of a fluorescent dye and PTB biomarkers. Octyl methacrylate-based monoliths with desirable retention and elution characteristics were chosen and used for on-chip fluorescent labeling of three PTB biomarkers. Purification of on-chip labeled samples was done by selective elution of unreacted dye prior to sample. Automated and rapid on-chip fluorescent labeling was achieved with similar efficiency to that obtained for samples labeled off chip. Additionally, protocols for microchip electrophoresis of several off-chip-labeled PTB biomarkers were demonstrated in poly(methyl methacrylate) microfluidic devices. This study is an important step toward the development of integrated on-chip labeling and separation microfluidic devices for PTB biomarkers.

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Advances in monoliths and related porous materials for microfluidics

Cited by 34 publications

References 104 publications

Sequence-specific DNA solid-phase extraction in an on-chip monolith: Towards detection of antibiotic resistance genes

Sequence-specific DNA solid-phase extraction in an on-chip monolith: Towards detection of antibiotic resistance genes

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

On-chip fluorescent labeling using reversed-phase monoliths and microchip electrophoretic separations of selected preterm birth biomarkers

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