A New Stability-Indicating Rp-HPLC Method for Determination of Curcumin: An Application to Nanoparticulate Formulation

Abstract

Objective: The present study was aimed to develop a rapid, accurate, linear, sensitive and stability-indicating high performance liquid chromatographic method for determination of curcumin and to implement the developed method for the estimation of curcumin in the nanoparticulate formulation. Methods:Method development was performed using various solvent, buffer-solvent ratios, at different flow rates for better resolution and to decrease the run time. The developed method was validated in accordance with the international conference on harmonization (ICH) guidelines. The developed method was implemented to estimate the amount of curcumin in the curcumin-nanoparticulate formulation. Results:The optimum chromatographic conditions with adequate resolution for curcumin (16.10 min) was achieved when the separation was carried using C18 Conclusion:The developed analytical method is simple, precise, and reproducible and thus can be used for stability-indicating analysis of curcumin in pharmaceutical formulations. column at ambient temperature with an isocratic elution mode of mobile phase composed of a degassed mixture of phosphate buffer pH 3 and acetonitrile (50:50 v/v) at 1.0 ml/min flow rate with a total run time of 20 min. The developed method was validated for system suitability, accuracy, precision, limit of detection (LOD), limit of quantitation (LOQ), linearity and range. The LOD and LOQ were found to be 0.018 and 0.056 μg/ml respectively, which indicates that the method was sensitive, and can detect and quantify at lower levels of curcumin. Linearity range was from 5-15 μg/ml for curcumin with regression coefficient 0.997 indicates that at this concentration range curcumin was highly linear. Percent assay of curcumin was found to be 98.7% and curcumin recovered was found to be 0.78 mg which are estimated by using the developed method.

CURCUMINOID AND TOXICITY LEVELS OF ETHANOL EXTRACT OF JAVANESE GINGER (Curcuma xanthorriza) ON BRINE SHRIMP (Artemia salina) LARVAE AND ZEBRAFISH (Danio rerio) EMBRYOS

“…Curcuminoids are a group of phenylpropanoid compounds consisting of curcumin, desmethoxycurcumin, and bisdemethoxycurcumin [16]. In this study, the curcuminoid content in ethanol extract of Javanese ginger was determined using HPLC.…”

Development and Validation of Rp-HPLC Method for Simultaneous Estimation of Curcumin and Piperine

“…The method development for the simultaneous estimation of curcumin and piperine was performed using a different flow rate, elution mode, different mobile phase and buffer ratio [1,2].…”

“…Structurally, curcumin is 1,7-bis (4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione as shown in fig. 1 along with other two de-methoxy compounds which are desmethoxycurcumin and bisdemethoxycurcumin [1,2]. It possesses antioxidant, anti-inflammatory, anti-microbial and anticancer activity due to its diverse molecular targets [3,4].…”

Analytical Method Development and Validation for Simultaneous Estimation of Curcumin and Cyclosporine by Rp-HPLC

“…Chemically, curcumin is (1E, 6E)-1,7-bis (4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione [1]. Literature survey revealed LC-MS [2], RP-HPLC [2], HPTLC [3], stability indicating HPLC [4], and UV spectrophotometric [1,[5][6][7] methods for estimation of curcumin alone or in combination with other drugs in bulk, in dosage forms and human plasma. Cyclosporine is an immunosuppressant drug used in post allogenic organ transplant to reduce the activity of patient's immune system.…”