Development and Validation of Rp-HPLC Method for Simultaneous Estimation of Curcumin and Piperine

Khismatrao, Ankita; Bhairy, Srinivas; Hirlekar, Rajashree

doi:10.22159/ijap.2018v10i5.21140

Cited by 14 publications

(9 citation statements)

References 7 publications

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“…ATR-FTIR method has been developed for quantification of encapsulation efficiency of NSO by applying Beer-Lambert law after selecting the combinations of wavenumbers. The developed method was valid with linearity in the range of 25-300 mg/mL with R 2 =0.998, precision as expressed with relative standard deviation (RSD) of 8.4%, limit of detection of 0.28 mg NSO/mL and limit of quantification of 0.87 mg NSO/mL (Khismatrao et al, 2018).…”

Section: Authentication Of Nigella Sativa Oilmentioning

confidence: 91%

Nigella sativa oil: physico-chemical properties, authentication analysis and its antioxidant activity

Rohman¹,

Lukitaningsih²,

Rafi³

et al. 2019

Food Res.

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Nigella sativa oil (NSO) is one of the high value oils in fats and oils industry due to its nutritional applications and its beneficial effects on human health. Several biological activities have been reported, especially antioxidant activities due to its active components, especially phenolics compounds. Some methods have been used for extraction of NSO from seeds to obtain high yield with excellent quality which includes solvent extraction, cold press, Soxhlet, and microwave assisted extraction. NSO commands a high price in the market, as a consequence, NSO is a target to be adulterated with cheaper oils such as corn and soybean oils. Indeed, the authentication analysis of NSO by determining several physico-chemical properties to determine the characteristics of NSO must be performed. This review highlighted some physico-chemical properties of NSO along with authentication of NSO from adulterants. The antioxidant activities of NSO were also highlighted in this review. Based on its activity as antioxidant, NSO is a good source to be used in nutraceutical and pharmaceutical products.

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Section: Authentication Of Nigella Sativa Oilmentioning

confidence: 91%

Nigella sativa oil: physico-chemical properties, authentication analysis and its antioxidant activity

Rohman¹,

Lukitaningsih²,

Rafi³

et al. 2019

Food Res.

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“…1.36gm of potassium dihydrogen phosphate and 2ml of triethylaminewere transferred into a beaker containing 1000ml of water and sonicated to dissolve the contents completely. The pH was adjustedto 3.5±0.05 with Orthophosphoric acid and mixed and filtered through 0.45µ filter paper [13].…”

Section: Preparation Of Buffer Mobile Phase and Diluentsmentioning

confidence: 99%

Analytical Method Development and Validation and Forced Degradation Stability-Indicating Studies of Favipiravir by RP-HPLC and UV in Bulk and Pharmaceutical Dosage Form

Ali¹,

Mobina²,

Mehfuza³

et al. 2021

JPRI

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Aims: To develop and validate a new, simple, rapid, precise, and accurate An Eco-friendly RP-HPLC and UV-Method Development and Validation for an estimation of Favipiravir in Bulk and pharmaceutical dosage form followed by Forced Degradation Studies. Study Design: This was employed for UV-visible (200-400 nm and 400-800 nm respectively) and RP-HPLC method development using C 18 inertsil column and optimization of variables for Favipiravir estimation in bulk and formulations. Place and Duration of the Study: The present work was carried out at Ali-allana College of Pharmacy, Akkalkuwa between the duration of November-2020 to February-2021. Methodology: UV-Spectroscopic method was developed for the estimation of Favipiravir in the bulk and pharmaceutical dosage form. The solvent selected for the Favipiravir UV analysis was water, the solution in a range of 2-10µg/ml was scanned in the UV region from 200-400 nm and the λmax value was determined. The RP-HPLC method was developed on inertsil ODS-3V C18 150 mm x 4.6mm x 5μ column using buffer pH 3.5: acetonitrile [90:10] as mobile phase at flow rate 1.0 ml/min and PDA detection at 358 nm. Results: The maximum absorbance was observed at 358 nm. The wavelength 358 nm was selected for further analysis of Favipiravir. The calibration curve was determined using drug concentrations ranging from 2-10 µg/ml. The % recovery for accuracy was 100.50-100.76%. The method was to be precise with a % RSD value 0.51-1.37 and 0.77-1.78 for intraday and Interday respectively. The limit of detection (LOD) and limit of quantification (LOQ) was found to be 0.0723 &0.219 µg/ml respectively by UV method. The RP-HPLC method was shown to be linear in the 50-250 μg/ml concentration range. The limit of detection (LOD) and limit of quantification (LOQ) was found to be 2.186 & 6.626 μg/ml respectively. The method was to be precise with a % RSD value 0.25-1.53 and 0.86-1.68 for intraday and inter-day respectively. Conclusion: Here we conclude that the developed UV and RP-HPLC methods are precise, accurate, sensitive, and reproducible for the quantitative estimation of Favipiravir bulk and its formulation. The developed method can be used by the pharmaceutical industries for the routine analysis of Favipiravir, in particular by UV and RP-HPLC. The main features of the proposed method are economic and eco-friendly with less retention time around 5.0 min.

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“…Linearity was assessed by measuring several analyte concentrations varying quantities of standard stock solution was diluted with the SLS (4% w/v) solution to give a 5, 10, 15, 20, and 25 µg/ml concentration. The calibration curve was obtained by plotting the absorbance against concentration (µg/ml) [17].…”

Section: A) Linearitymentioning

confidence: 99%

An Eco-friendly RP-HPLC and UV-Method Development and Validation for an Estimation of Tolvaptan in Bulk and Tablet Dosage form Followed by Forced Degradation Studies

A¹,

Nazifa²,

Mobina³

et al. 2021

JPRI

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Aims: To develop and validate a new, simple, rapid, precise, and accurate An Eco-friendly RP-HPLC and UV-Method Development and Validation for an estimation of Tolvaptan in Bulk and Tablet dosage form followed by Forced Degradation Studies Place and Duration of the Study: The present work has been carried out at Ali-Allana College of Pharmacy, Akkalkuwa between November-2020 to April-2021. Methodology: The UV-Spectroscopic method was developed for the estimation of tolvaptan in bulk and tablet dosage form. The solvent selected for the tolvaptan UV analysis was 4% aq. SLS solution, the solution of 10µg/ml was scanned in UV region from 200-400 nm and the λmax value was determined. The RP-HPLC method was developed on Sunsil C18 150 mm x 4.6mm x 5μ column using acetonitrile: water [45:55] as mobile phase at flow rate 1.0 ml/min and UV detection at 266 nm. Results: The maximum absorbance was observed at 266 nm. The wavelength 266 nm was selected for further analysis of tolvaptan. The calibration curve was determined using drug concentrations ranging from 20-100 µgm/ml. The system suitability was performed by injecting a standard solution containing 200µg/ml of tolvaptan in six replicates. For two of them, the peak asymmetric were <1.5 and the theoretical plate number was >2000, and the %RSD of tolvaptan was less than 2. Conclusion: From the above results, it was concluded that the developed UV and RP-HPLC methods are precise and accurate and can be applied for the quantitative estimation of tolvaptan from bulk and tablet dosage forms. The method can be used for routine testing of tolvaptan by the pharmaceutical industry. Validation of the developed method was done as per International Conference on Harmonization (ICH) Q2R1 guidelines.

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Development and Validation of Rp-HPLC Method for Simultaneous Estimation of Curcumin and Piperine

Cited by 14 publications

References 7 publications

Nigella sativa oil: physico-chemical properties, authentication analysis and its antioxidant activity

Nigella sativa oil: physico-chemical properties, authentication analysis and its antioxidant activity

Analytical Method Development and Validation and Forced Degradation Stability-Indicating Studies of Favipiravir by RP-HPLC and UV in Bulk and Pharmaceutical Dosage Form

An Eco-friendly RP-HPLC and UV-Method Development and Validation for an Estimation of Tolvaptan in Bulk and Tablet Dosage form Followed by Forced Degradation Studies

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