A new serological technique, time-resolved fluoroimmunoassay (TRFIA), for the immunological diagnosis of urinary schistosomiasis

Aceti, Antonio; Pennica, Alfredo; Celestino, D; Paparo, Samuele Barbaro; Caferro, Marta; S, Sanguigni; Marangi, M; Sebastiani, A.

doi:10.1016/0035-9203(88)90158-7

Cited by 12 publications

(7 citation statements)

References 13 publications

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“…Obviously, TRFIA for the detection of galectin-3 is superior to ELISA because of its higher sensitivity. Our Wnding is in agreement with many previous studies on the detection of other biomolecules (Aceti et al 1988;Maple et al 2001). The greater sensitivity of TRFIA beneWts from the use of a highly detectable "tracer" and the sensitivity of labeled reagent techniques may be greatly increased by improving the signal-to-noise ratio.…”

Section: Discussionsupporting

confidence: 89%

The expressions and clinical significances of tissue and serum galectin-3 in pancreatic carcinoma

Xie

Chen

et al. 2012

J Cancer Res Clin Oncol

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Section: Discussionsupporting

confidence: 89%

The expressions and clinical significances of tissue and serum galectin-3 in pancreatic carcinoma

Xie

Chen

et al. 2012

J Cancer Res Clin Oncol

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“…The time-resolved¯uorescent assay has also been used to detect CA in other parasitic diseases, e.g. schistosomiasis and angiostrongyliasis (Aceti et al 1988;de Jonge et al 1989;Shih and Chen 1991).…”

Section: Development Of Detection Systemmentioning

confidence: 99%

The detection and occurrence of circulating antigens of Trichinella spiralis during worm development

Li¹,

Ko²

2001

Parasitol Res

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Four enzyme-based immunoassays were compared in detecting circulating antigens (CA) of Trichinella spiralis, i.e. microfluorescence, dissociated enhanced lanthanide fluoroimmunoassay (DELFIA), enhanced chemiluminescence and enzyme-linked immunosorbent assay (ELISA). Parameters which could affect the sensitivity and specificity of the assays were evaluated. Different combinations of polyclonal antibody (PA) and five monoclonal antibodies (mAbs) against the excretory/secretory antigens of the nematode were tested to produce an optimal antigen-detecting system. DELFIA and a "sandwich" consisting of PA as capturing antibody and mAb 7C2C5 as detecting antibody, yielded the most stable and sensitive results; and 1 ng CA/ml could be detected. The assay did not cross-react with heterologous antigens of Angiostrongylus cantonensis, Ascaris suum, Cysticercus cellulosae, Fasciolopsis buski, Gnathostoma hispidum and Trichuris suis. Fluctuating levels of CA were observed in the serum of experimentally infected mice at various periods post-infection. As early as days 4 and 6, a significant amount of CA was detected. The level reached a peak at day 10, then declined and another peak was observed at day 18. The monitoring of the corresponding antibody response by ELISA showed that IgM was first detected at day 10, reaching a peak at day 16. A marked increase in IgG1 was noted from day 16 and its level was significantly higher than that of IgG2.

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“…For the detection of various diagnostic trace protein markers, TRFIA is a promising and appealing approach. Compared with traditional ELISA, TRFIA has been proposed as a more sensitive and specific approach [Aceti et al, ]. In this paper, a reliable and sensitive indirect TRFIA was developed and evaluated for the determination of EBNA1 IgA antibody in human serum.…”

Section: Introductionmentioning

confidence: 99%

Development of a time‐resolved fluorescence immunoassay for Epstein–Barr virus nuclear antigen 1‐immunoglobulin A in human serum

Chen

Liu

Liang

et al. 2015

Journal of Medical Virology

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Enzyme-linked immunosorbent assay (ELISAs) specific for Epstein-Barr virus nuclear antigen 1 (EBNA1)-immunoglobulin A (IgA) are most commonly used in the clinical diagnosis of EBV infection. But they have a low sensitivity and the enzyme-labeled antibodies are unstable. In this study, a novel immunoassay based on an indirect time-resolved fluoroimmunoassay (TRFIA) was developed. Microtiter plates were coated with recombinant EBNA1. We used Eu(3) (+)-labeled anti-human IgA as probe. The precision, sensitivity, specificity, and stability were evaluated, and comparison with traditional and commercially available ELISAs was also made. The cut-off value for our TRFIA was 2.7. Intra- and inter-assay coefficients of variation for the TRFIA were 1.56-4.99% and 3.92-6.95%, respectively; whereas those for the ELISA were 4.54-8.16% and 7.07-10.52%, respectively. Sensitivity was obviously better than traditional ELISA when diluted positive samples serially. Additionally, stability, specificity test and comparison of sensitivity and specificity between the TRFIA and commercial ELISAs all proved satisfactory. In conclusion, the results demonstrated that EBNA1 IgA TRFIA was a sensitive immunoassay and had potential value in large-scale screening of human serum samples in developing countries.

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A new serological technique, time-resolved fluoroimmunoassay (TRFIA), for the immunological diagnosis of urinary schistosomiasis

Cited by 12 publications

References 13 publications

The expressions and clinical significances of tissue and serum galectin-3 in pancreatic carcinoma

The expressions and clinical significances of tissue and serum galectin-3 in pancreatic carcinoma

The detection and occurrence of circulating antigens of Trichinella spiralis during worm development

Development of a time‐resolved fluorescence immunoassay for Epstein–Barr virus nuclear antigen 1‐immunoglobulin A in human serum

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